Rational engineering of recombinant picornavirus capsids to produce safe, protective vaccine antigen

Foot-and-mouth disease remains a major plague of livestock and outbreaks are often economically catastrophic. Current inactivated virus vaccines require expensive high containment facilities for their production and maintenance of a cold-chain for their activity. We have addressed both of these major drawbacks. Firstly we have developed methods to efficiently express recombinant empty capsids. Expression constructs aimed at lowering the levels and activity of the viral protease required for the cleavage of the capsid protein precursor were used; this enabled the synthesis of empty A-serotype capsids in eukaryotic cells at levels potentially attractive to industry using both vaccinia virus and baculovirus driven expression. Secondly we have enhanced capsid stability by incorporating a rationally designed mutation, and shown by X-ray crystallography that stabilised and wild-type empty capsids have essentially the same structure as intact virus. Cattle vaccinated with recombinant capsids showed sustained virus neutralisation titres and protection from challenge 34 weeks after immunization. This approach to vaccine antigen production has several potential advantages over current technologies by reducing production costs, eliminating the risk of infectivity and enhancing the temperature stability of the product. Similar strategies that will optimize host cell viability during expression of a foreign toxic gene and/or improve capsid stability could allow the production of safe vaccines for other pathogenic picornaviruses of humans and animals

Expression of Foot-and-Mouth Disease Virus Capsid Proteins in Silkworm-Baculovirus Expression System and Its Utilization as a Subunit Vaccine

Background: Foot-and-mouth disease (FMD) is a highly contagious disease of livestock that causes severe economic loss in susceptible cloven-hoofed animals. Although the traditional inactivated vaccine has been proved effective, it may lead to a new outbreak of FMD because of either incomplete inactivation of FMDV or the escape of live virus from vaccine production workshop. Thus, it is urgent to develop a novel FMDV vaccine that is safer, more effective and more economical than traditional vaccines. Methodology and Principal Findings: A recombinant silkworm baculovirus Bm-P12A3C which contained the intact P1-2A and 3C protease coding regions of FMDV Asia 1/HNK/CHA/05 was developed. Indirect immunofluorescence test and sandwich-ELISA were used to verify that Bm-P12A3C could express the target cassette. Expression products from silkworm were diluted to 30 folds and used as antigen to immunize cattle. Specific antibody was induced in all vaccinated animals. After challenge with virulent homologous virus, four of the five animals were completely protected, and clinical symptoms were alleviated and delayed in the remaining one. Furthermore, a PD50 (50 % bovine protective dose) test was performed to assess the bovine potency of the subunit vaccine. The result showed the subunit vaccine could achieve 6.34 PD50 per dose

Expression and functional characterization of a single chain FV antibody directed against secretions involved in plant nematode infection process.

International audienc

Reducing noise impact and improving quality of life by addressing annoyance

Aircraft noise is the most significant impact on communities residing near airports. But in addition to acoustical factors, non-acoustical factors also contribute to noise annoyance. In the ANIMA project, relevant indicators influencing the quality of life of residents living near an airport are identified. Literature studies, but also focus group meetings, workshops, and online surveys for communities near altogether nine different European airports are performed to understand the role of the airport and the implications on the well-being of citizens. Furthermore, it is intended to study what current communication strategies of the airports are and how they influence the impact of aircraft noise on residents’ annoyance response and well-being. A detailed literature study as well as a re-analysis of data has been carried out to develop awakenings indicators for nocturnal fly-overs, as noise-induced sleep disturbance has been linked to adverse health effects and is also associated with annoyance. Another study will be conducted to understand human visual perception of aircraft and their noise levels using a noise simulator with Virtual Reality glasses, where different aircraft types and their noise impact will be compared in a laboratory study and later in an “in situ” experiment. Finally, people’s perception of environmental sound near the airport and the relation with land use planning will be evaluated by developing a mobile application to be used around Heathrow and Ljubljana Airport. Together with a carefully crafted social media study, data on property-value and movement of people throughout the day, we hope to establish more evidence on the relationship between land-use planning, noise perception and quality of life. The outcomes of all studies will be tested later in intervention studies and, or integrated into a Best-Practice portal that will be available for airports, governments, communities, and other stakeholders