Application of paired student t-test on impact of Anti-retroviral therapy on CD4 cell count among HIV Seroconverters in serodiscordant heterosexual relationships: A case study of Nyanza region, Kenya.

Human immunodeficiency virus (HIV) infection leads to rise in HIV-RNA resulting in CD4 T-cell decline leading to AIDS-related illness. Knowing the effect of Antiretroviral Therapy (ART) on CD4 cell count is vital in assessing the progression of the disease and treatment planning for treatment.  This study sought to apply paired t-test distribution to assess the effect of CD4 cell count just before and after initiation of ART among HIV infected individuals. The target populations were HIV sero-converters enrolled in a prospective randomized placebo controlled trial in Nyanza region, Kenya.  CD4 cell count was measured at the time of sero-conversion and subsequently after very six months of follow up. Participants were referred for initiation of ART at patient support centre once the criteria for initiation was met and report back the ART regime they were put on and the date they were started on .  We applied paired t-test to assess the change in CD4 cell count after initiating ART. Use of ART within a median time of 9 months resulted rise in CD4 cell count by 241 cells per ul, 95% CI (60-422) which confirms the effect of ART in protecting depletion of CD4 cell count. Keywords: Sero-converters, Progression, ART, HIV, CD4 cells, t-test

A study of the medicinal plants used by the Marakwet Community in Kenya.

BACKGROUND: The medicinal plants used by herbalists in Kenya have not been well documented, despite their widespread use. The threat of complete disappearance of the knowledge on herbal medicine from factors such as deforestation, lack of proper regulation, overexploitation and sociocultural issues warrants an urgent need to document the information. The purpose of the study was to document information on medicinal plants used by herbalists in Marakwet District towards the utilization of indigenous ethnobotanical knowledge for the advancement of biomedical research and development. METHODS: Semi- structured oral interviews were conducted with 112 practicing herbalists. The types of plants used were identified and the conditions treated recorded. RESULTS: Herbal practice is still common in the district, and 111 plants were identified to have medicinal or related uses. Different herbal preparations including fruits and healing vegetables are employed in the treatment of various medical conditions. Veterinary uses and pesticides were also recorded. CONCLUSION: The study provides comprehensive ethnobotanical information about herbal medicine and healing methods among the Marakwet community. The identification of the active ingredients of the plants used by the herbalists may provide some useful leads for the development of new drugs

Ethnopharmacological survey of the medicinal plants used in Tindiret, Nandi County, Kenya

Background: An inventory of the medicinal plants used by herbalists to treat various ailments in Tindiret area of Nandi County, Kenya was conducted by a team comprising of medical researchers and botanists with a view to preserve indigenous ethnobotanical knowledge and potential research and development of new drugs.Materials and Methods: Data was collected from practicing herbalists through semi-structured interviews, group discussions and observations. The types of medical conditions treated, treatment methods as well as the plants used were recorded.Results and Conclusion: A total of 50 medicinal plants belonging to 28 families used for the treatment of 32 medical conditions were recorded. The disease conditions which were treated by the highest number of plants were abdominal/colic pains with 15 different plants recorded (13%), respiratory disorders 15(13%) and infertility14 (12%). The most widely used medicinal plants were Vernonia amygdalina 7(7%), Toddalia asiatica 6(6%), Aloe kedongensis 5(5%) and Zanthoxylum gilletii 5(5%); whereas the families with the highest number of plant usage were Fabaceae 8(14%), Lamiaceae 4(8%) and Solanaceae 4(8%) plants respectively. The study documents important ethno-medical knowledge on the plants used by herbalists in Tindiret sub-county.Keywords: Ethnopharmacology, Indigenous knowledge, Traditional medicine, Research, Conservatio

ETHNOPHARMACOLOGICAL SURVEY OF THE MEDICINAL PLANTS USED IN TINDIRET, NANDI COUNTY, KENYA

Background: An inventory of the medicinal plants used by herbalists to treat various ailments in Tindiret area of Nandi County, Kenya was conducted by a team comprising of medical researchers and botanists with a view to preserve indigenous ethnobotanical knowledge and potential research and development of new drugs. Materials and Methods: Data was collected from practicing herbalists through semi-structured interviews, group discussions and observations. The types of medical conditions treated, treatment methods as well as the plants used were recorded. Results and Conclusion: A total of 50 medicinal plants belonging to 28 families used for the treatment of 32 medical conditions were recorded. The disease conditions which were treated by the highest number of plants were abdominal/colic pains with 15 different plants recorded (13%), respiratory disorders 15(13%) and infertility14 (12%). The most widely used medicinal plants were Vernonia amygdalina 7(7%), Toddalia asiatica 6(6%), Aloe kedongensis 5(5%) and Zanthoxylum gilletii 5(5%); whereas the families with the highest number of plant usage were Fabaceae 8(14%), Lamiaceae 4(8%) and Solanaceae 4(8%) plants respectively. The study documents important ethno-medical knowledge on the plants used by herbalists in Tindiret sub-county

The healthcare system and client failures contributing to maternal mortality in rural Kenya

Background The global maternal mortality ratio is estimated at 211/100 000 live births in 2017. In Kenya, progress on reducing maternal mortality appears to be slow and persistently higher than the global average, despite efforts by the government’s provision of free maternity services in both private and public facilities in 2013. We aimed to explore and describe the experiences of midwives on maternal deaths that are associated with the healthcare system and client failures in Migori, Kenya. Methods An explanatory, qualitative approach method was adopted. In-depth interviews were conducted with the purposively selected midwives working in peripartum units of the three sampled hospitals within Migori County in Kenya. The hospitals included two county referral hospitals and one private referral hospital. Saturation was reached with 37 respondents. NVivo 11 software was used for analysis. Content analysis using a qualitative approach was adopted. Accordingly, the data transcripts were synthesised, coded and organised into thematic domains. Results Identified sub-themes: sub-optimal care, staff inadequacy, theatre delays, lack of blood and essential drugs, non-adherence to protocols, staff shortage, inadequate equipment and supplies, unavailable ICU wards, clients’ ANC non-adherence. Conclusion In conclusion, the study notes that the healthcare system and client failures are contributing to maternal mortality in the study setting. The major failures are across the pregnancy continuum starting from antenatal care, and intrapartum to post-natal care. This can illustrate that some pregnant mothers are getting sub-optimal care reducing their survival chances. To reduce maternal mortality in Migori County, the key highlighted healthcare system and client failures should be addressed through a multidisciplinary approach mechanism.Health Studie

CD26/dipeptidyl peptidase IV (CD26/DPPIV) is highly expressed in peripheral blood of HIV-1 exposed uninfected Female sex workers

<p>Abstract</p> <p>Background</p> <p>Design of effective vaccines against the human immunodeficiency virus (HIV-1) continues to present formidable challenges. However, individuals who are exposed HIV-1 but do not get infected may reveal correlates of protection that may inform on effective vaccine design. A preliminary gene expression analysis of HIV resistant female sex workers (HIV-R) suggested a high expression CD26/DPPIV gene. Previous studies have indicated an anti-HIV effect of high CD26/DPPIV expressing cells in vitro. Similarly, high CD26/DPPIV protein levels in vivo have been shown to be a risk factor for type 2 diabetes. We carried out a study to confirm if the high CD26/DPPIV gene expression among the HIV-R were concordant with high blood protein levels and its correlation with clinical type 2 diabetes and other perturbations in the insulin signaling pathway.</p> <p>Results</p> <p>A quantitative CD26/DPPIV plasma analysis from 100 HIV-R, 100 HIV infected (HIV +) and 100 HIV negative controls (HIV Neg) showed a significantly elevated CD26/DPPIV concentration among the HIV-R group (mean 1315 ng/ml) than the HIV Neg (910 ng/ml) and HIV + (870 ng/ml, p < 0.001). Similarly a FACs analysis of cell associated DPPIV (CD26) revealed a higher CD26/DPPIV expression on CD4+ T-cells derived from HIV-R than from the HIV+ (90.30% vs 80.90 p = 0.002) and HIV Neg controls (90.30% vs 82.30 p < 0.001) respectively. A further comparison of the mean fluorescent intensity (MFI) of CD26/DPPIV expression showed a higher DPP4 MFI on HIV-R CD4+ T cells (median 118 vs 91 for HIV-Neg, p = 0.0003). An evaluation for hyperglycemia, did not confirm Type 2 diabetes but an impaired fasting glucose condition (5.775 mmol/L). A follow-up quantitative PCR analysis of the insulin signaling pathway genes showed a down expression of NFκB, a central mediator of the immune response and activator of HIV-1 transcription.</p> <p>Conclusion</p> <p>HIV resistant sex workers have a high expression of CD26/DPPIV in tandem with lowered immune activation markers. This may suggest a novel role for CD26/DPPIV in protection against HIV infection in vivo.</p

Participatory Farmer Evaluation of Sorghum Cultivars with Improved Drought Tolerance and Enhanced Utilization Characteristics

Sorghum is the main cereal crop grown in semi-arid areas of Eastern Kenya including Mbeere district, used for food, fodder, fuel, and thatching. Drought tolerance is the most important criteria when deciding on a sorghum cultivar to grow. The presentation provides an overview of research carried out with farmer groups in Mbeere district to determine which cultivars would best meet their needs

Participatory Farmer Evaluation of Sorghum Cultivars with Improved Drought Tolerance and Enhanced Utilization Characteristics

This paper reports on a study which allowed farmers to evaluate new sorghum cultivars with drought tolerant capacity that would also meet the farmers’ utilization and marketing preferences. Farmers assessed two of the cultivars, namely, KARI/Mtama-1 and Gatururu, as of higher utility value that the brown-seeded cultivar, Serena. They noted that the latter grew well but is less palatable and marketable

Worldwide interconnections of Africa using crops as historical and cultural markers

The historical, social, and economical importance of precolonial connections between Africa and the rest of the world has been undervalued. In the present study, we use crops as historical and social markers to analyze intercontinental connections from the perspective of Kenyan and Ugandan regions northeast of Lake Victoria. Crops were inventoried in 148 small farms from 74 localities, using successively free listing, to reveal their socio-cultural salience, and a closed list method, for a more complete picture of the agricultural, environmental and social diversity. The total sample included 75 crops (30 African, 21 Asian, 21 American, and 3 European). Among farms, crop richness varied from 6 to 32. It was higher in Uganda than in Kenya, and lowest around the Winam Gulf. The 12 American crops introduced at Renaissance were uniformly distributed, and the observed structure was mostly due to differences in African and Asian crop richness. In terms of crop frequency, exotic crops account for 74%, with 46% for American crops. The 14 most frequent crops included 10 from America, 3 from Asia, and 1 for Africa, with negligible differences among linguistic groups. Consistently, the free listing citation order demonstrated the high cultural salience of American crops. The spatial distribution of minor crops suggest differential diffusion among linguistic groups, which could be further studied using linguistic approaches on crop names