Primer sequences used for quantitative real-time PCR.

*<p>reference genes used for normalization.</p

Experimental Design.

<p>Animals were vaccinated twice with TT. Directly before (0), as well as 2, 4, 8, 24 and 75 hours after each vaccination blood samples were collected. A final blood sample was taken at day 28. Pairwise comparisons of the reference time 0 hours against each following sampling time point were set up for the identification of DE-genes. * reference time point.</p

Top 20 canonical pathways within the first 24 h of immune response after the first vaccination.

<p>1 Cellular Immune Response.</p><p>2 Cytokine Signaling.</p><p>3 Pathogen-Influenced Signaling.</p><p>4 Cellular Growth, Proliferation and Development.</p><p>5 Intracellular and Second Messenger Signaling.</p><p>6 Nuclear Receptor Signaling.</p><p>7 Cell Cycle Regulation.</p><p>8 Cellular Stress and Injury.</p><p>9 Organismal Growth and Development.</p><p>10 Growth Factor Signaling.</p><p>11 Neurotransmitters and Other Nervous System Signaling.</p><p>12 Cardiovascular Signaling.</p><p>13 Ingenuity Toxicity List Pathways.</p><p>n.s. not significant.</p

Most affected biofunctions within 24 hours after the first and after the second vaccination.

<p>Segments of the respective bars for the first and the second vaccination represent the relative frequencies of IPA biofunctional categories superior to canonical pathways that are most significant at 2 h, 4 h, 8 h and 24 h after immunization.</p

Number, direction and q-values [34] of DE-genes (p<0.05) over the time points of blood sampling after vaccination.

<p>Number, direction and q-values <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0058306#pone.0058306-Wattrang1" target="_blank">[34]</a> of DE-genes (p<0.05) over the time points of blood sampling after vaccination.</p

Significant pathways affected by differential gene expression at embryonic stages and D35.

<p>Up (U) or Down (D) regulation of DEG associated with each comparison (versus control) are separated within each thermal modification: increase (H) or decrease (L) in incubation temperature during ED7-10 (H10 and L10) or ED10-13 (H13 and L13). Significant pathways are grouped into eight major categories (sc.): sc.1 cell maintenance, proliferation, differentiation, and replacement; sc.2 organismal organ and tissue development; sc.3 nutrient metabolism; sc.4 genetic information and nucleic acid processing; sc.5 molecular transport; sc.6 cell signaling and interaction; sc.7 small molecule biochemistry; and sc.8 response to stimuli and associated. The—log (<i>p</i> values) related with significant pathways (Benjamini Hochberg corrected) are plotted in green (small) to red (large).</p

Major gene networks at ED10 and ED13.

<p>For H10ΔC a network related to tissue development and skeletal muscle development and function was derived (A). For L13ΔC a network related to skeletal-muscular and connective tissue development was derived (B). Red color, up-regulated; Green color, down-regulated.</p

The lay apostolate in the spirituality of the third Carmelite order

ENGLISH ANNOTATION The Lay Apostolate of the Third Carmelite Order Spirituality The Second Vatican Council came up with a new interpretation of the layperson as well as the lay apostolate. Every person of the people of God, i.e. a layperson too, shares responsibility for the redemptive mission of the whole Church. The task of the members of the Third Carmelite Order is to live the charism of Carmel in the world, the apostolate being a part of their spirituality. The fundamental and also specific feature of the apostolate of the Third Carmelite Order is the contemplative attitude, taken and lived in normal life conditions, in the world. Every their prayer, service, testimony as well as community result from the experience of contemplation. All of these features are typical of the Carmelite charism. They also represent a specific form or area of the apostolate of the members of the Third Carmelite Order. Key words: Second Vatican Council, Vatican II Layperson Apostolate Third Carmelite Order Spiritualit

Differentially expressed genes for each treatment condition relative to controls within embryonic (A) stages and at D35 (B) and between embryonic stages and D35 within the same treatments (C-F) (blue embryonic, red adult).

<p>Intersection areas show numbers of common DEGs and their direction of regulation in each stage.</p

Major gene networks at D35.

<p>For L10ΔC a network related to gene expression, cell signaling and cellular function and maintenance pathways was derived (A). For L13ΔC a network related to organ, embryonic and skeletal-muscular system development and function was derived (B). Red color, up-regulated; Green color, down-regulated.</p