How the techniques of molecular biology are developed from natural systems

A striking characteristic of the highly successful techniques in molecular biology is that they are derived from natural occurring systems. RNA interference (RNAi), for example, utilises a mechanism that evolved in eukaryotes to destroy foreign nucleic acid. Other examples include restriction enzymes, the polymerase chain reaction, fluorescent proteins and CRISPR-Cas9. I propose that natural molecular mechanisms are exploited by biologists for their effectors’ (protein or nucleic acid) activity and biological specificity (protein or nucleic acid can cause precise reactions). I also show that the developmental trajectory of novel techniques in molecular biology, such as RNAi, is four characteristic phases. The first phase is discovery of a biological phenomenon. The second is identification of the mechanism’s trigger(s), the effector and biological specificity. The third is the application of the technique. The final phase is the maturation and refinement of the molecular biology technique. The development of new molecular biology techniques from nature is crucial for both biological and biomedical research

How the techniques of molecular biology are developed from natural systems

A striking characteristic of the highly successful techniques in molecular biology is that they are derived from natural systems. RNA interference (RNAi), for example, utilises a mechanism that evolved in eukaryotes to destroy foreign nucleic acid. Other examples include restriction enzymes, the polymerase chain reaction, green fluorescent protein and CRISPR-Cas. I propose that biologists exploit natural molecular mechanisms for their effectors’ (protein or nucleic acid) activity and biological specificity (protein or nucleic acid can cause precise reactions). I also show that the developmental trajectory of novel techniques in molecular biology, such as RNAi, is four characteristic phases. The first phase is discovery of a biological phenomenon, typically as curiosity driven research. The second is identification of the mechanism’s trigger(s), the effector and biological specificity. The third is the application of the technique. The final phase is the maturation and refinement of the molecular biology technique. The development of new molecular biology techniques from nature is crucial for biological research. These techniques transform scientific knowledge and generate new knowledge

Exercise Recommendations for Cardiac Patients with Chronic Nonspecific Low Back Pain

Musculoskeletal comorbidities (MSKCs) are the most frequent cause of activity limitations in persons with cardiovascular disease (CVD) and affect as many as 70% of this population. It has been observed that over 50% of new outpatient cardiac rehabilitation participants experience some musculoskeletal pain, with back pain reported by up to 38% of cardiac rehabilitation patients. Back pain can limit performance of activities of daily living (ADLs) and reduce exercise tolerance and compliance during outpatient cardiac rehabilitation (CR). This article will describe ways to facilitate CR exercise participation in patients who have comorbid, chronic nonspecific low back pain (CNSLBP) and have been medically cleared to exercise

How the techniques of molecular biology are developed from natural systems

A striking characteristic of the highly successful techniques in molecular biology is that they are derived from natural systems. RNA interference (RNAi), for example, utilises a mechanism that evolved in eukaryotes to destroy foreign nucleic acid. Other examples include restriction enzymes, the polymerase chain reaction, green fluorescent protein and CRISPR-Cas. I propose that biologists exploit natural molecular mechanisms for their effectors’ (protein or nucleic acid) activity and biological specificity (protein or nucleic acid can cause precise reactions). I also show that the developmental trajectory of novel techniques in molecular biology, such as RNAi, is four characteristic phases. The first phase is discovery of a biological phenomenon, typically as curiosity driven research. The second is identification of the mechanism’s trigger(s), the effector and biological specificity. The third is the application of the technique. The final phase is the maturation and refinement of the molecular biology technique. The development of new molecular biology techniques from nature is crucial for biological research. These techniques transform scientific knowledge and generate new knowledge

Licensing of nominal ellipsis in Hungarian possessives

We argue, based on novel data, that the possessor head (Poss) can license ellipsis of its complement in Hungarian. That is, contra existing claims in the literature, possessor morphology can survive nominal ellipsis and be stranded on the remnant. Adopting Saab & Lipták (2016)’s of ellipsis licensing, we propose that there is variation in the size of the ellipsis site in Hungarian: nominal ellipsis can be licensed by either Num or Poss. We further propose that nominal ellipsis licensed by Poss can capture a previously unanalyzed variation in the Hungarian possessive pronoun paradigm. Specifically, the two variants of possessive pronouns correspond to two different structures: one is the anaphoric possessive (see Dékány 2015), while the other exists only as a consequence of nominal ellipsis, which, as we show, is a productive possibility

On the meaning of intonational contours: a view from scalar inference

This paper investigates the meaning of intonational contours by experimentally testing how they affect the likelihood of scalar inference (SI) calculation. Our main test case is the rise-fall-rise contour (RFR) which, based on prior theoretical work, is predicted to either increase or decrease the likelihood of SI. We conducted two experiments using an inference task: one where participants first produce a target sentence with their choice of contour and one where participants listen to a pre-recorded target sentence with a particular contour. The experiments converged in showing that the RFR increases SI rate relative to a neutral fall. Additionally, production data revealed the frequent use of another contour that resembles the Contradiction Contour, which we label Concession Contour. This contour also led to an increase in SI rate, although to a lesser extent than the RFR. In addition to informing the theoretical literature on RFR, our results also highlight the methodological importance of controlling for intonation in the study of SI

Exploring the connection between Question Under Discussion and scalar diversity

Previous research has revealed that different scalar expressions give rise to scalar inferences (SIs) at different rates. This variation has been termed scalar diversity. In this study, we investigate the role of Questions Under Discussion (QUDs) in explaining this variation in SI rates. Investigating 43 different scalar expressions, we first show that explicit QUDs robustly affect calculation rates: questions based on the stronger of two scalar terms lead to higher SI rates than questions promoting the weaker one. Second, we explore whether the likelihood of asking the stronger question in general (Question Availability) can explain the scalar diversity effect. Our results suggest that Question Availability is indeed a predictor of scalar diversity, but only for scales where both terms denote intervals (unbounded scales), and not for scales where the stronger member has a fixed meaning (bounded scales)

Quantifying semantic and pragmatic effects on scalar diversity

Scalar inference (SI), the process by which we systematically infer meanings stronger than what was explicitly said, has long been a central topic of investigation in semantics-pragmatics. A recent experimental finding that has generated interest is scalar diversity: that the robustness of SI calculation varies across lexical scales. For instance, the some but not all SI is much more likely to arise than good but not excellent. In this paper, we take a first step toward more rigorously quantifying the observed variation across scales using relative entropy. We then turn to the question of how factors independent of scalar diversity can make SI calculation both more likely and more uniform. We find that a supportive discourse context and overt exhaustification with the focus particle only both increase inference rates and reduce variation across scales, with the effect of only being stronger. However, there still remains a lot of scalar diversity; only when we combine context with semantic exhaustification do we find uniformity across lexical scales

Degree estimates as a measure of inference calculation

Scalar inference (SI), e.g., utterances containing the quantifier some being enriched to mean some but not all, is a central topic in semantics-pragmatics. Of recent interest in the experimental literature is the phenomenon of scalar diversity: that different lexical scales exhibit variation is how likely they are to lead to SI. However, studies of scalar diversity have almost exclusively relied on a particular experimental task: the inference task. In this paper, we argue that the inference task suffers from a number of shortcomings: namely, that it biases by providing participants with the stronger alternative and that it obscures pragmatic inferences other than SI. Instead we offer as an alternative a degree estimate task to investigate utterances containing scalar terms. We use the degree estimate task to reassess previous inference task-based findings from the literature on how two manipulations (discourse context and only) affect the likelihood of inference calculation. Our results show that the two tasks produce results that differ from each other in subtle but important ways

Tracking the activation of scalar alternatives with semantic priming

From an utterance of Mary ate some of the deep dish, hearers frequently infer that Mary didn’t eat all of the deep dish. Similarly, an utterance of The movie is good might lead hearers to conclude that the movie isn’t excellent. These inferences are instances of scalar implicature (SI). The standard assumption is that SI arises via hearers’ reasoning about alternative utterances that the speaker could have said, but did not. In particular, hearers are taken to consider stronger alternatives such as all (or Mary ate all of the deep dish) and excellent (or The movie is excellent) and derive their negation. In this study, we investigate the psycholinguistic reflexes of this inferential process. We use semantic priming with lexical decision to test whether lexical alternatives such as all and excellent are retrieved and activated in the processing of SI-triggering sentences. The results of our experiments indeed suggest that alternatives play a role in the processing of SI, though a number of empirical puzzles remain