Growth and Fatty Acid Composition of Marine Microalga Nannochloropsis SP in Medium Enriched with Magnesium

Micro-algae are to be an attractive way to produce bio-diesel due to high photosynthetic yields and lipid accumulation in cells. This high productivity combined with possibility to uptake CO2 stimulated its utilization as a biological mitigation method of CO2, at once as an alternative renewable source of energy. Growth characteristics and chemical composition of micro-algae can be altered by culture environment. Nutrient sufficiency,included magnesium element (Mg2+) is important factors on overall biochemical composition. In study, Nannochloropsis sp was cultivated in Erlenmeyer 250 ml containing 200 ml f/2 medium. There are three groups of treatment with different levelof magnesium (Mg2+), i.e. 0 (M0); 0.1mgL-1 (M1); and 1.0 mgL-1 (M2). All treatment was designed triplicate in batch system. Culture was then aerated continuously with sterile atmospheric air (1.5 L.min-1). Cells were harvested on 25th day after inoculation and analyzed. Data showed that Chlorophyll-a increased linearly with time and maximum at 18th days of growth period, i.e. 23.57; 26.44; and 27.74mgL-1, for M0; M1; and M2,respectively. Chlorophyll-a content decreased significantly when pH dropped to 5-6.Enrichment with Mg2+ increased the chlorophyll-a content 12.2-17.7%. Dry cell reached 375-400mgL-1 in all treatment. Lipid content of Nannochloropsis sp in control (M0) is 55.3%, higher than M1 and M2. Saturated fatty acid tends to increase from 80.70 (M0)to 96.70 (M1) and 94.53% (M2). Fatty acid of M0 and M1 was composed dominantly by palmitic acid (C16:0), i.e. 49.19-70.75% total fatty acids. Meanwhile, M2 treatment was dominantly by lauric acid (C12:0), i.e. 32.98%

Zona Wisata Kawasan Wisata Alam Air Terjun Madakaripura, Kabupaten Probolinggo

Kabupaten Probolinggo memiliki keindahan wisata alam salah satunya berupa wisata air terjun. Wisata Alam Air Terjun Madakaripura termasuk dalam kawasan hutan lindung dan rawan bencana longsor sehingga pengembangannya membutuhkan pembagian zona wisata yang sesuai dengan karakteristik fisik kawasan wisata alam. Penelitian ini bertujuan menentukan zona wisata kawasan wisata alam Air Terjun Madakaripura, Kabupaten Probolinggo. Metode analisa yang digunakan dalam tahapannya adalah analisa Theoritical Deskriptif Kualitatif, teknik analisa Delphi dan analisa teknik Overlay. Hasil penelitian ini berupa zona wisata pada kawasan wisata alam Air Terjun Madakaripura, Kabupaten Probolinggo dengan melihat pada kondisi eksisting serta faktor-faktor yang mempengaruhi pengembangan kawasan wisata

Additional file 5: of A novel short L-arginine responsive protein-coding gene (laoB) antiparallel overlapping to a CadC-like transcriptional regulator in Escherichia coli O157:H7 Sakai originated by overprinting

Phylogenetic analysis of ECs5115 by the Maximum Likelihood method. The tree with the highest log likelihood (− 61,042.2643) is shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. Initial tree(s) for the heuristic search were obtained by applying the Neighbor-Joining method to a matrix of pairwise distances estimated using the Maximum Composite Likelihood (MCL) approach. A discrete Gamma distribution was used to model evolutionary rate differences among sites (5 categories; +G, parameter = 0.5123). The rate variation model allowed some sites to be evolutionarily invariable (+I, 32.8153% sites). The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 30 nucleotide sequences. All positions containing gaps and missing data were eliminated. There was a total of 8025 positions in the final dataset. Evolutionary analyses were conducted in MEGA6. In the alignment to the right, start codons are highlighted in blue and stop codons with a red asterisk. (PPTX 614 kb

Additional file 8: Table S6. of Differentiation of ncRNAs from small mRNAs in Escherichia coli O157:H7 EDL933 (EHEC) by combined RNAseq and RIBOseq – ryhB encodes the regulatory RNA RyhB and a peptide, RyhP

Data set of the 115 annotated ncRNAs and their properties, including blastp hits and proposed Shine-Dalgarno sequences. (XLSX 44 kb