The continued value of disk diffusion for assessing antimicrobial susceptibility in clinical laboratories: Report from the Clinical and Laboratory Standards Institute Methods Development and Standardization Working Group

Expedited pathways to antimicrobial agent approval by the U.S. Food and Drug Administration (FDA) have led to increased delays between drug approval and the availability of FDA-cleared antimicrobial susceptibility testing (AST) devices.</jats:p

Risk and resiliency factors in posttraumatic stress disorder

BACKGROUND: Not everyone who experiences a trauma develops posttraumatic stress disorder (PTSD). The aim of this study was to determine the risk and resiliency factors for this disorder in a sample of people exposed to trauma. METHOD: Twenty-five people who had developed PTSD following a trauma and 27 people who had not were asked to complete the Posttraumatic Stress Diagnostic Scale, the Coping Inventory for Stressful Situations, and the State-Trait Anxiety Inventory. In addition, they completed a questionnaire to provide information autobiographic and other information. ANALYSIS: Five variables that discriminated significantly between the two groups using chi-square analysis or t-tests were entered into a logistic regression equation as predictors, namely, being female, perceiving a threat to one's life, having a history of sexual abuse, talking to someone about the event, and the "intentionality" of the trauma. RESULTS: Only being female and perceiving a threat to one's life were significant predictors of PTSD. Taking base rates into account, 96.0% of participants with PTSD were correctly classified as having the disorder and 37.0% of participants without PTSD were correctly classified as not having the disorder, for an overall success rate of 65.4% CONCLUSIONS: Because women are more likely than men to develop PTSD, more preventive measures should be directed towards them. The same is true for trauma victims (of both sexes) who feel that their life was in dange

SPATIAL ANALYSIS OF SOIL FERTILITY MANAGEMENT USING INTEGRATED HOUSEHOLD AND GIS DATA FROM SMALLHOLDER KENYAN FARMS

Although soil fertility is recognized as a primary constraint to agricultural production in developing countries, use of fertilizer in Sub-Saharan Africa is declining. Smallholder farmers still rely heavily on livestock manure for soil fertility management. To explore the determinants of soil fertility management practices, including both the use of cattle manure and inorganic fertilizer, data are used from a sample of 3,330 geo-referenced farm households across Central and Western Kenya. A bivariate probit model is applied to jointly examine the use of the two technologies. Particular attention is given to measures of location related to market access and agroclimate, which in the adoption literature have typically been addressed using crude proxies. To avoid such proxies, GIS-derived variables are integrated into the household decision model. Their use also allows the spatial prediction of uptake based on parameter estimates. The results show clearly the derived-demand nature of soil fertility services, based on markets for farm outputs. They also illustrate that supply of manure for soil fertility amendments is conditioned by demand for livestock products, especially milk. The integration of GIS-derived variables is shown to better estimate the effects of location than the usual measures employed, and offers scope to wider use in technology adoption research.spatial analysis, soil fertility, market access, technology adoption., Farm Management, Land Economics/Use, Q12, Q16,

Mechanisms of linezolid resistance among coagulase-negative staphylococci determined by whole-genome sequencing.

UnlabelledLinezolid resistance is uncommon among staphylococci, but approximately 2% of clinical isolates of coagulase-negative staphylococci (CoNS) may exhibit resistance to linezolid (MIC, ≥8 µg/ml). We performed whole-genome sequencing (WGS) to characterize the resistance mechanisms and genetic backgrounds of 28 linezolid-resistant CoNS (21 Staphylococcus epidermidis isolates and 7 Staphylococcus haemolyticus isolates) obtained from blood cultures at a large teaching health system in California between 2007 and 2012. The following well-characterized mutations associated with linezolid resistance were identified in the 23S rRNA: G2576U, G2447U, and U2504A, along with the mutation C2534U. Mutations in the L3 and L4 riboproteins, at sites previously associated with linezolid resistance, were also identified in 20 isolates. The majority of isolates harbored more than one mutation in the 23S rRNA and L3 and L4 genes. In addition, the cfr methylase gene was found in almost half (48%) of S. epidermidis isolates. cfr had been only rarely identified in staphylococci in the United States prior to this study. Isolates of the same sequence type were identified with unique mutations associated with linezolid resistance, suggesting independent acquisition of linezolid resistance in each isolate.ImportanceLinezolid is one of a limited number of antimicrobials available to treat drug-resistant Gram-positive bacteria, but resistance has begun to emerge. We evaluated the genomes of 28 linezolid-resistant staphylococci isolated from patients. Multiple mutations in the rRNA and associated proteins previously associated with linezolid resistance were found in the isolates investigated, underscoring the multifocal nature of resistance to linezolid in Staphylococcus. Importantly, almost half the S. epidermidis isolates studied harbored a plasmid-borne cfr RNA methylase gene, suggesting that the incidence of cfr may be higher in the United States than previously documented. This finding has important implications for infection control practices in the United States. Further, cfr is commonly detected in bacteria isolated from livestock, where the use of phenicols, lincosamides, and pleuromutilins in veterinary medicine may provide selective pressure and lead to maintenance of this gene in animal bacteria

Radio Jet-Ambient Medium Interactions on Parsec Scales in the Blazar 1055+018

As part of our study of the magnetic fields of AGN we have recently observed a large sample of blazars with the Very Long Baseline Array. Here we report the discovery of a striking two-component jet in the source 1055+018, consisting of an inner spine with a transverse magnetic field, and a fragmentary but distinct boundary layer with a longitudinal magnetic field. The polarization distribution in the spine strongly supports shocked-jet models while that in the boundary layer suggests interaction with the surrounding medium. This behavior suggests a new way to understand the differing polarization properties of strong- and weak-lined blazars.Comment: LaTex; 10 pages; 6 figures; reference fix; to appear in ApJL, 518, 1999 June 2

Research that matters: Outcome mapping for linking knowledge to poverty-reduction actions

An 'Outcome Mapping' approach was applied retrospectively to five diverse, highly collaborative research projects aimed at poverty reduction. Designed to help plan for, clarify, and document intended and actual changes in behaviour, actions, and relationships of groups and organisations that directly influence a project's intended beneficiaries, Outcome Mapping enabled us to identify and describe the strategies and actions that played important roles in the innovations achieved. Successful strategies observed included the use of champions, jointly producing high-profile outputs that enhanced the status of local partners, multiple communication strategies, targeting ongoing policy processes, and strong emphases on and investment in capacity building

Farmer Perception of Technology and its Impact on Technology Uptake: The Case of Fodder Legume in Central Kenya Highlands.

Technology adoption by farmers is crucial to increasing agricultural productivity hence meeting food and nutrition challenges in Africa. Economists investigating consumer demand have accumulated considerable evidence showing that consumers generally have subjective preferences for product attributes. However, when investigating adoption of new agricultural technologies, economists have lagged behind in analysing how farmers' (the consumer of agricultural technologies) subjective perceptions of technology characteristics affect their adoption decisions. Focusing on farmer perceptions of technologies may provide a better understanding of technology adoption since they deal with the technologies and probably perceive technologies differently from researchers and extension agents. The objective of this paper is to investigate farmers' perception of technology and its impact on adoption using a case study of legume forages in central Kenya highlands. Data from a random sample of 131 farm households in four districts in central Kenya was used. Using participatory techniques, four most important fodder legume attributes to farmers in their adoption decision were identified. These were then used in conjoint analysis. An ordered probit model was estimated to assess relative importance of each attribute to the farmer. A tobit model was also estimated to show the effect of farmers' perception of calliandra and desmodium on probability and intensity of adoption. Results showed that dry season tolerance and economy on land are most important characteristics of fodder legumes to the farmers. It was also found that Calliandra and desmodium were more relevant to the farmers in the area than other fodders. Farmers' perception of the two fodders had a significant impact on their adoption. Consequently, it was recommended that before introducing a technology in an area, it is necessary that the farmers' perception of the technology be analysed Conjoint analysis, ordered probit and tobit estimates, fodder legume adoption.Research and Development/Tech Change/Emerging Technologies,

Evidence-Based Practice Confidence and Behavior Throughout the Curriculum of Four Physical Therapy Education Programs: A Longitudinal Study

Background Evidence-based practice (EBP) is a foundational process taught in health professional education, yet it is unclear when EBP confidence and skills are obtained. Increases in EBP confidence and behaviors from the start of physical therapy programs to post graduation have been reported in studies that evaluated a single program or used non-valid questionnaires. This study aimed to describe changes in EBP confidence and behavior using validated questionnaires of students from four physical therapy education programs throughout their curriculum and one year post graduation. Methods One hundred and eighty-one students from a potential pool of 269 (67.3%) consented to participate. Students completed the Evidence-Based Practice Confidence (EPIC) Scale and the Evidence-Based Practice Implementation Scale (EBPIS) at 6 timepoints: start of the program, prior to first clinical experience, after first clinical experience, at the end of classroom instruction, graduation, and one year post. Medians (Mdn) and 25th and 75th percentiles (P25, P75) were calculated for 42 (23.2%) students with complete data across all timepoints. Change between timepoints was assessed using Friedman’s test and Wilcoxon signed rank test with a Bonferroni correction for post hoc analysis. Results There were significant changes in EPIC scores (p \u3c 0.001) from enrollment (Mdn 50.0, P25, P75 35.5, 65.9) to prior to first clinical experience (Mdn 65.5, P25, P75 57.3, 72.5) and after the first clinical experience (Mdn 67.3, P25, P75, 58.9, 73.2) to the end of classroom instruction (Mdn 78.6, P25, P75, 72.0, 84.1). Significant increases on the EBPIS (p \u3c 0.01) were only seen from after the first year of training (Mdn 15, P25, P75, 10.0, 22.5) to end of the first clinical experience (Mdn 21.5, P25, P75 12.0, 32.0). Conclusions EBP confidence increased significantly after classroom instruction but remained the same after clinical experiences and at one year post graduation. EBP behavior significantly increased only after the first clinical experience and remained the same through graduation. Confidence and behavior scores were higher than were previously reported in practicing professionals. Ongoing assessment of EBP confidence and behavior may help instructors build appropriate curricula to achieve their outlined EBP objectives