Occurrence of Phthalate Esters in Coffee and Risk Assessment

Coffee, one of the most widely consumed beverages in the world, is commercialized as powder and beans in different types of packaging and extracted through several methods. In this regard, the present study focused on evaluating the concentration of two of the most used phthalates in plastic materials (bis(2-ethylhexyl)phthalate (DEHP) and di-butyl phthalate (DBP)) in coffee powder and beverages to assess their migration from different packaging and machines. Furthermore, the levels of exposure to these endocrine disruptors in regular coffee consumers were estimated. Samples of packaged coffee powder/beans (n = 60) from different forms of packaging (multilayer bag, aluminum tin, and paper pod) and coffee beverages (n = 40) that were differently extracted (by professional espresso machine (PEM), Moka pot (MP), and home espresso machine (HEM)) were analyzed by extraction of the lipid fraction, purification, and determination by gas chromatography–mass spectrometry (GC/MS). Risk due to consumption of coffee (1–6 cups) was assessed based on tolerable daily intake (TDI) and incremental lifetime cancer risk (ILCR). No significant differences emerged in DBP and DEHP concentrations among different types of packaging (multilayer, aluminum, and paper), whereas higher levels of DEHP were reported in beverages extracted by PEM (6.65, 2.58–11.32) than by MP (0.78, 0.59–0.91) and HEM (0.83, 0.62–0.98). The presence of higher DEHP levels in coffee beverages than in coffee powder may be due to its leaching through machine components. However, the levels of PAEs did not exceed the specific migration limits (SMLs) set out for food contact materials (FCM), and exposure to PAEs from coffee beverages was low, justifying the small risk due of its consumption. Consequently, coffee can be considered a safe beverage for exposure to some phthalic acid esters (PAEs)

Acrylamide in Baby Foods: A Probabilistic Exposure Assessment

Acrylamide (also known as 2-propenamide) (AA) is a toxicant that develops in food during high-temperature cooking, and its occurrence is common in biscuits and baked snacks. AA is known for its in vivo neurotoxic and carcinogenic effects, and it is considered a potential carcinogen for humans. Infants may be exposed to AA as early as during weaning through baked food such as biscuits. This study set out to ascertain the concentration of AA in food products intended for infants to assess the dietary exposure to this food contaminant. AA levels were determined through GC/MS and bromination, and dietary exposure was evaluated by a probabilistic method based on Monte Carlo simulation. The results showed that the probability of a carcinogenic exposure is 94%, 92%, and 87%, respectively, for 6-, 12-, and 18-months infants, suggesting the need to delay the introduction of baked products in the diet of weaned infants. It should be noted, however, that these conclusions were drawn considering the biscuits as the primary source of exposure

Level of Biogenic Amines in Red and White Wines, Dietary Exposure, and Histamine-Mediated Symptoms upon Wine Ingestion

Biogenic amines (BAs) are involved in physiological processes. Foods where typically high levels of BAs occur are fermented food and beverage. This work set out to evaluate the occurrence of BAs in red and white wines, and to also ascertain the dietary exposure to BAs among consumers. Besides, a case report of a probable histamine intoxication upon ingestion of contaminated wine was described. The samples were analyzed through derivatization with dansyl chloride and HPLC-UV detection. Red wines showed higher levels of BAs, especially putrescine (PUT) and histamine (HIS), than white wines (median concentrations of 7.30 and 2.45 mg/L, respectively). However, results of our investigation showed that the dietary exposure to BAs through the consumption of wine (red and white) were lower than the recommended maximum levels for the acute exposure to HIS and tyramine (TYR). In contrast, the levels of BAs in wine on tap were much higher than in bottled wine and close to recommended values. The levels of HIS, TYR, and PUT in tap wine of 9.97, 8.23, and 13.01 mg/L, respectively, were associated with histamine-mediated symptoms in six young individuals after consumption of about three glasses of wine. The overall results and multivariate analysis confirm that red wine shows a higher concentration of BAs than white wine, especially putrescine and histamine. This finding is attributable to the malolactic fermentation that is common for most red wine production. It is also evident that incorrect preservation processes can lead to an increase in BA levels, probably due to the action of bacteria with high decarboxylase activity. The exposure values, although below the toxicity thresholds, could lead to histamine-mediated symptoms in susceptible individuals, also according to the case report discussed in this study

Giant subcutaneous lipomatosis in Myoclonic Epilepsy with Ragged Red Fibers syndrome: The first literature report of "laparoscopic" excision

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LDOC1 expression in fibroblasts of patients with Down syndrome

Abstract Down syndrome (DS) is characterised by intellectual disability and is caused by trisomy 21. Apoptosis is a programmed cell death process and is involved in neurodegenerative diseases such as Alzheimer. People with DS can develop some traits of Alzheimer disease at an earlier age than subjects without trisomy 21. The leucine zipper, down regulated in cancer 1 (LDOC1) appears to be involved in the apoptotic pathways. The aim of the present work was to detect the presence of intracellular synthesis of LDOC1 protein and LDOC1 mRNA in fibroblast cultures from DS subjects. The western blot shows the presence of LDOC1 protein in fibroblasts of DS subjects but no evidence of LDOC1 protein in fibroblasts of normal subjects. LDOC1 gene mRNA expression is increased in fibroblasts from DS subjects compared to fibroblasts from normal subjects. The data obtained from this study strengthen the hypothesis that the over-expression of LDOC1 gene could play a role in determining the phenotype of individuals with DS but does not exclude that this results from apoptotic mechanisms

Analysis of Rooftop Photovoltaics Diffusion in Energy Community Buildings by a Novel GIS- and Agent-Based Modeling Co-Simulation Platform

The present work introduces an empirically ground agent-based modeling (ABM) framework to assess the spatial and temporal diffusion of rooftop photovoltaic (PV) systems on existing buildings of a city district. The overall ABM framework takes into account social, technical, environmental, and economic aspects to evaluate the diffusion of PV technology in the urban context. A city district that includes 18 720 households distributed over 1 290 building blocks and a surface area of 2.47 km2 is used to test the proposed ABM framework. Results show how the underlying regulatory framework (i.e., the rules of the internal electricity market) influences the pattern and intensity of adoption, thus realizing different shares of the available potential. Policies that support the establishment of `prosumers' within Condominiums (i.e., energy community buildings), and not in single-family houses only, is key to yield high diffusion rates. The installed capacity increases by 80% by switching from the one-to-one configuration to the one-to-many paradigm, i.e., from 5.90 MW of rooftop PV installed on single-family households and/or single PV owners to 10.64 MW in energy community buildings. Moreover, the possibility to spread the auto-generated solar electricity over the load profile of the entire population of Condominium results in self-consumption rates greater than 50% and self-sufficiency ratios above 20% for the majority of the simulated buildings

considerazioni su un caso di actinomicosi cervicale

Actinomycosis is a bacterial infection due to Gram positive bacteria of Actinomyces genus. Actinomyces israelii is the most common in human infection.The authors described a case occurred in a 43 aged woman with a clinical presentation of a right submandibular indurative mass.After surgery, histological examination showed a granulomatous tissue with many actinomycetes. Patient was submitted to treatment with i.v penicillin. On the 11th day antibiotic therapy was replaced with ceftriaxone and teicoplanina. Patient did not present intercurrence during this period and presented improvement of the lesion.Actinomycosis is an uncommon disease. Establishment of the definite diagnosis requires a high index of suspicion in all cases of relapsing cervical lesions. Penicillin is effective and it can be replaced by third-generation cephalosporins

A Distributed Platform for Multi-modelling Co-simulations of Smart Building Energy Behaviour

Nowadays, buildings are responsible of a large consumption of energy in our cities. Moreover, buildings can be seen as the smallest entity of urban energy systems. On these premises, in this paper we present a flexible and distributed co-simulation platform that exploits a multi-modelling approach to simulate and evaluate energy performance in smart build- ings. The developed platform exploits the Mosaik co-simulation framework and implements the Functional Mock-up Interface (FMI) standard in order to couple and synchronise heterogeneous simulators and models. The platform integrates: i) the thermal performance of the building simulated with EnergyPlus, ii) the space heating and hot water system modelled as an heat pump with PID control strategy in Modelica, and iii) different Python models used to simulate household occupancy, electrical loads, roof-top photovoltaic production and smart meters. The platform guaranties a plug-and-play integration of models and simulators, hence, one or more models can be easily replaced without affecting the whole simulation engine. Finally, we present a demonstration example to test the functionalities and capabilities of the developed platform, and discuss future developments of our framework

Coffee Silverskin: Chemical and Biological Risk Assessment and Health Profile for Its Potential Use in Functional Foods

The coffee supply chain is characterized by a complex network with many critical and unsustainable points producing a huge amount of waste products. Among these, coffee silverskin (CS), the only by-product of the coffee roasting phase, has an interesting chemical profile that suggests potential use as a food ingredient. However, few data on its safety are available. For this reason, the purpose of the study was to assess the occurrence of chemical and biological contaminants in CS, and the resulting risk due to its potential consumption. Essential, toxic, and rare earth elements, polycyclic aromatic hydrocarbons (PAHs), process contaminants, ochratoxin A (OTA), and pesticides residues were analyzed in three classes of samples (Coffea arabica CS, Coffea robusta CS, and their blend). Furthermore, total mesophilic bacteria count (TMBC) at 30 C, Enterobacteriaceae, yeasts, and molds was evaluated. The risk assessment was based upon the hazard index (HI) and lifetime cancer risk (LTCR). In all varieties and blends, rare earth elements, pesticides, process contaminants, OTA, and PAHs were not detected except for chrysene, phenanthrene, and fluoranthene, which were reported at low concentrations only in the arabica CS sample. Among essential and toxic elements, As was usually the most representative in all samples. Microorganisms reported a low load, although arabica and robusta CS showed lower contamination than mixed CS. Instead, the risk assessment based on the potential consumption of CS as a food ingredient did not show either non-carcinogenic or carcinogenic risk. Overall, this study provides adequate evidence to support the safety of this by-product for its potential use in functional foods

GAMES: a General-purpose Architectural model for Multi-Energy System engineering applications

The growing interest in Multi-Energy Systems (MES) leads the scientific community to implement innovative technologies to analyse and simulate these complex systems. Two main research trends are identified in such analysis: i) improve the usability and capability of preexisting reference architectures in the energy field to cope with high-level use case descriptions, and ii) study the interoperability of such reference architectures in order to increase systematic and functional analysis of MES use cases. GAMES is a a general-purpose architectural model for MES engineering application. The aim is twofold: i) GAMES implements an extension of Smart Grid Architecture Model (SGAM) to cope with MES use case descriptions, and ii) it offers a methodology to deal with a systemic description of the use case through a combination of UML and SysML integrated in the proposed architectural model. Furthermore, GAMES will allow the implementation of Domain Specific Language (DSL) and hardware configuration for the specific components described by UML/SysML diagrams. Compared to other solutions, GAMES allows to assess both research trends in a single hierarchical ICT infrastructure