Antioxidants and Anticholinesterase Activities of the Characterized Ethanolic of Ripe Sesoot (Garcinia picrorrhiza Miq.) Fruit Extract (GpKar) and Xanthone

Oxidative stress has been known to contribute to Alzheimer’s disease. Acetylcholinesterase (AChE) enzyme may lead to Alzheimer’s disease as a neurotransmitter. Antioxidants may have protective activities against oxidative damage and Alzheimer’s disease. Acetylcholinesterase inhibitors also can be used in the treatment of various neurological disorders for management of Alzheimer’s disease. This study aimed to determine antioxidant and anticholinesterase effects of Garcinia picrorrhiza Miq. fruit extract (GpKar) and its compounds, xanthone. Antioxidant activity was measured by H2O2 scavenging inhibitory activity, while anticholinesterase activity was measured using modified Ellman method. GpKar has higher H2O2 scavenging inhibitory activity (IC50= 967.28 µg/ml) compared to xanthone (IC50= 1198.95 µg/ml). In the anticholinesterase inhibitory activity, GpKar has lower activity (IC50= 70.25 µg/ml) compared to xanthone (11.80 µg/ml). In summary, GpKar has higher antioxidant activity but lower anticholinesterase activity compared to its compounds, xanthone. However, GpKar has potency as antioxidant agent to prevent Alzheimer’s disease

Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit as antiaging

Introduction: Nitric oxide (NO) is a free radical that belongs to reactive nitrogen species (RNS). The excess amount of NO in body generates physical changing on skin as a consequence of alteration in connective tissue through formation of lipid peroxides, cell content, and enzymes. These free radicals induce damage to extracellular matrix (ECM) and are responsible in reducing skin elasticity. Antioxidants possess significant role in delaying aging process by scavenging free radicals and preventing collagenase and elastase enzymes activities. This study aimed to evaluate antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit (GpKar) as antiaging remedy. Methods: Antioxidant activity was performed by NO scavenging activity assay, while anti-aging activity was performed through inhibitory effects of collagenase and elastase activities. Results: In antioxidant activity, GpKar had lower NO scavenging activity (IC50=1530.34 µg/ mL) compared to xanthone (IC50=85.40 µg/mL). In collagenase inhibitory activity, GpKar also had lower inhibition collagenase activity (IC50= 1169.31 µg/mL) compared to xanthone (IC50= 286.32 µg/mL). In elastase inhibitory activity, GpKar had lower inhibition elastase activity (IC50= 152.93 µg/mL) compared to xanthone (IC50= 21.26 µg/mL). Conclusion: In summary, GpKar and its compounds possess antioxidant, anticollagenase, and antielastase activities for antiaging, and might be beneficial in these subjects

Antioxidants and Anticholinesterase Activities of the Characterized Ethanolic of Ripe Sesoot (Garcinia picrorrhiza Miq.) Fruit Extract (GpKar) and Xanthone

Oxidative stress has been known to contribute to Alzheimer’s disease. Acetylcholinesterase (AChE) enzyme may lead to Alzheimer’s disease as a neurotransmitter. Antioxidants may have protective activities against oxidative damage and Alzheimer’s disease. Acetylcholinesterase inhibitors also can be used in the treatment of various neurological disorders for management of Alzheimer’s disease. This study aimed to determine antioxidant and anticholinesterase effects of Garcinia picrorrhiza Miq. fruit extract (GpKar) and its compounds, xanthone. Antioxidant activity was measured by H2O2 scavenging inhibitory activity, while anticholinesterase activity was measured using modified Ellman method. GpKar has higher H2O2 scavenging inhibitory activity (IC50= 967.28 µg/ml) compared to xanthone (IC50= 1198.95 µg/ml). In the anticholinesterase inhibitory activity, GpKar has lower activity (IC50= 70.25 µg/ml) compared to xanthone (11.80 µg/ml). In summary, GpKar has higher antioxidant activity but lower anticholinesterase activity compared to its compounds, xanthone. However, GpKar has potency as antioxidant agent to prevent Alzheimer’s disease

In-vitro studies on antioxidant and antidiabetic potential of Sesoot (Garcinia picrorrhiza Miq.) fruit ethanolic extract from Indonesia

Diabetes mellitus (DM) is a disease that can be identified by high levels of blood glucose. Garcinia plants have been widely used for many traditional medicines as antioxidant, anticancer, antidiabetics, and antiinflammation. The antioxidant and antidiabetic activities of (Garcinia picrorrhiza Miq.) or sesoot fruit extract were evaluated in this study and compared with xanthone. The antioxidant and antidiabetic of ethanolic ripe sesoot (G. picrorrhiza Miq.) fruit extract (GpKar) was evaluated by (ABTS) reducing activity, α-glucosidase, β-glucosidase, and α-amylase inhibitor activity. GpKar showed higher ABTS-reducing activity (IC50 = 49.30 µg/mL) than xanthone (IC50 = 404.30 µg/mL). GpKar showed IC50 = 109.32 µg/mL for α-glucosidase inhibitory activity, while xanthones had a better activity (IC50 = 33.97 µg/mL). GpKar also showed lower α-amylase inhibitory activity and β-glucosidase (IC50 = 126.01 and 9432.09 µg/mL) compared to xanthone (IC50 = 44.32 and 405.03 µg/mL, respectively). The compounds of GpKar are proven to have antioxidant and antidiabetic activities. Therefore, it will be industrially relevant to develop a natural medicine for decreasing DM risk, thus evaluating the antioxidant and antidiabetic effect of G. picrorrhiza by a pre-clinic study is needed

Antioxidant and Antiaging Properties of Ethanolic Ripe Sesoot Fruit Extract

Skin aging can be characterized by changes in skin, such as the appearance of wrinkles and loss of skin moisture. Some elements that might lead to cell damages and aging are free radicals through the increase of the activities of hyaluronidase and tyrosinase. Bioactive compounds found in sesoot (Garcinia picrorhiza Miq.) are known for their antioxidant properties, which may reduce excessive amounts of free radicals in the body and act as an antiaging agent by inhibiting hyaluronidase and tyrosinase activities. This study aimed to examine the antioxidant, anti-hyaluronidase, and anti-tyrosinase properties found in Ethanolic Extract Ripe Sesoot (EERS). The ethanol extract of ripe sesoot fruit obtained from LIPI, Bogor Botanical Garden was used for the antioxidant and antiaging assays conducted at PT Aretha Medika Utama in December 2016. The activity of antioxidants was measured as the Ferric Reducing Antioxidant Power (FRAP) of the EERS, while the antiaging assays were performed through the inhibition activity of hyaluronidase and tyrosinase. Results indicated that the EERS has a higher FRAP activity (17.58 μM Fe (II)/μg) than xanthone (2.54 μM Fe (II)/μg) at the highest concentration of sample of 5,000 µg/mL. The anti-hyaluronidase of the EERS exhibited lower activity (IC50 of 619.21±12.15 µg/mL) than xanthone (IC50 of 365.55±25.10 µg/mL) and the tyrosinase inhibitory assay demonstrated a lower activity of EERS (IC50 of 1060.68±12.81 µg/mL) compared to xanthone (IC50 of 218.33±9.73 µg/mL). To conclude, EERS shows antioxidant and antiaging properties

Antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit as antiaging

Introduction: Nitric oxide (NO) is a free radical that belongs to reactive nitrogen species (RNS). The excess amount of NO in body generates physical changing on skin as a consequence of alteration in connective tissue through formation of lipid peroxides, cell content, and enzymes. These free radicals induce damage to extracellular matrix (ECM) and are responsible in reducing skin elasticity. Antioxidants possess significant role in delaying aging process by scavenging free radicals and preventing collagenase and elastase enzymes activities. This study aimed to evaluate antioxidants, anticollagenase and antielastase potentials of ethanolic extract of ripe sesoot (Garcinia picrorrhiza Miq.) fruit (GpKar) as antiaging remedy. Methods: Antioxidant activity was performed by NO scavenging activity assay, while anti-aging activity was performed through inhibitory effects of collagenase and elastase activities. Results: In antioxidant activity, GpKar had lower NO scavenging activity (IC50=1530.34 μg/mL) compared to xanthone (IC50=85.40 μg/mL). In collagenase inhibitory activity, GpKar also had lower inhibition collagenase activity (IC50= 1169.31 μg/mL) compared to xanthone (IC50= 286.32 μg/mL). In elastase inhibitory activity, GpKar had lower inhibition elastase activity (IC50= 152.93 μg/mL) compared to xanthone (IC50= 21.26 μg/mL). Conclusion: In summary, GpKar and its compounds possess antioxidant, anticollagenase, and antielastase activities for antiaging, and might be beneficial in these subjects