Determination of fatty acid composition content in the herbal antidiabetic collections

It was determined the qualitative composition and the quantitative content of fatty acids in the herbal antidiabetic collection № 3, № 4, № 7, № 13, № 19, which are used in folk medicine for prevention and treatment of diabetes mellitus type 2 in Ukraine by GC/MS method. According to the results it was identified 8 fatty acids in the herbal antidiabetic collection № 3, 13 fatty acids in the herbal antidiabetic collection № 4, 12 fatty acids in the herbal antidiabetic collection № 7, 13 fatty acids in the herbal antidiabetic collection № 13 and 11 fatty acids in the herbal antidiabetic collection № 19. The saturated fatty acids were found to be dominated in the raw plant material. The results of the quantitative research showed that the herbal antidiabetic collection № 3 contains 16.13 mg/g of fatty acids, the herbal antidiabetic collection № 4 – 27.17 mg/g, the herbal antidiabetic collection № 7 – 31.98 mg/g, the herbal antidiabetic collection № 13 – 27.37 mg/g, the herbal antidiabetic collection № 19 – 18.79 mg/g. The GC/MS analysis of the fatty acid composition in the herbal antidiabetic collections has shown that this raw material has a lot of fatty acids, which can have a positive effect for the treatment and prevention of diabetes mellitus type 2

Дослідження вмісту карбонових кислот у рослинних зборах методом ВЕРХ

The aim of the study was to research the qualitative composition and to investigate the quantitative content of some carboxylic acids in the herbal mixtures with established hypoglycemic, hypolipidemic and antioxidant activity in previous studies in vivo. Materials and methods. Studies of carboxylic acid content in the herbal mixtures were performed by HPLC analysis using Agilent Technologies 1200 liquid chromatograph (USA). Identification and quantitative analysis were performed using standard solutions of carboxylic compounds (tartaric, pyruvic, isocitric, citric, succinic and fumaric acids). Conclusions. HPLC analysis of five samples of the herbal mixture with antidiabetic activity showed the presence of six carboxylic acids. The dominant acid in all samples was isocitric acid. Among the most important for the prevention and treatment of diabetes, high levels of succinic and fumaric acids have been identified and established. The obtained data indicate a correlation between the phytochemical composition of the studied herbal mixtures and their pharmacodynamics, which was previously establishedЦель. Целью работы было изучить качественный состав и исследовать количественное содержание некоторых карбоновых кислот в растительных сборах, что имеют установленную гипогликемическую, гиполипидемическую и антиоксидантную активность в предыдущих исследованиях in vivo. Материалы и методы. Исследование содержания карбоновых кислот в растительных сборах выполняли методом ВЭЖХ с помощью жидкостного хроматографа Agilent Technologies 1200 (США). Идентификацию и количественный анализ проводили с использованием стандартных растворов карбоновых соединений (винной, пировиноградной, изолимонной, лимонной, янтарной и фумаровой кислот). Результаты. По результатам хроматографического исследования было установлено, что в исследуемых образцах содержится в большом количестве изолимонная кислота, содержание которой составляет (45,22±0,04) мг/г в растительном сборе № 3, (63,65±0,06) мг/г в растительном сборе № 4, (7,51±0,02) мг/г в растительном сборе № 7, (2,54±0,01) мг/г в растительном сборе № 13 и (43,48±0,05) мг/г в растительном сборе № 19. Кроме этого было обнаружено высокое содержание янтарной кислоты, как важного регулятора митохондриальной дисфункции и фумаровой кислоты, как мощного иммуномодулирующего, противовоспалительного и антиоксидантного агента. Выводы. ВЭЖХ анализ пяти образцов растительных сборов с антидиабетической активностью показал наличие шести карбоновых кислот. Доминирующей кислотой во всех образцах была изолимонная кислота. Среди наиболее важных для профилактики и лечения сахарного диабета было идентифицировано и установлено высокое количественное содержание янтарной и фумаровой кислот. Полученные данные свидетельствуют о наличии корреляционной связи между фитохимическим составом изучаемых растительных сборов и их фармакодинамикой, установленной предварительноМета. Метою роботи було вивчити якісний склад та дослідити кількісний вміст деяких карбонових кислот у рослинних зборах, що мають встановлену гіпоглікемічну, гіполіпідемічну та антиоксидантну активність у попередніх дослідженнях in vivo. Матеріали та методи. Дослідження вмісту карбонових кислот у рослинних зборах виконували методом ВЕРХ за допомогою рiдинного хроматографа Agilent Technologies 1200 (США). Ідентифікацію та кількісний аналіз проводили з використанням стандартних розчинів карбонових сполук (винної, піровиноградної, ізолимонної, лимонної, бурштинової та фумарової кислот). Результати. За результатами хроматографічного дослідження було встановлено, що у досліджуваних зразках міститься в найбільшій кількості ізолимонна кислота, вміст якої становить (45,22±0,04) мг/г у рослинному зборі № 3, (63,65±0,06) мг/г у рослинному зборі № 4, (7,51±0,02) мг/г у рослинному зборі № 7, (2,54±0,01) мг/г у рослинному зборі № 13 та (43,48±0,05) мг/г у рослинному зборі № 19. Окрім цього було виявлено високий вміст бурштинової кислоти, як важливого регулятора мітохондріальної дисфункції та фумарової кислоти, як потужного імуномодулюючого, протизапального та антиоксидантного агента. Висновки. ВЕРХ аналіз п’яти зразків рослинних зборів з антидіабетичною активністю показав наявність шести карбонових кислот. Домінуючою кислотою у всіх зразках була ізолимонна кислота. Серед найбільш важливих для профілактики та лікування цукрового діабету було ідентифіковано та встановлено високий кількісний вміст бурштинової та фумарової кислот. Отримані дані свідчать про наявність кореляційного зв’язку між фітохімічним складом досліджуваних рослинних зборів та їх фармакодинамікою, що було встановлено попереднь

Analysis of fatty acid composition content in the plant components of antidiabetic herbal mixture by GC-MS

Medical plants and their combinations due to the wide range of biologically active substances can influence on various links of the pathogenetic mechanism of development of diabetes mellitus and its complications. One of such combinations is an anidiabetic herbal mixture (Urtica dioica L. leaf, Rosa majalis L. fruits, Vaccinium myrtillus L. leaf, Mentha piperita L. herb and Taraxacum officinale L. roots) with established hypoglycemic, hypolipidemic, antioxidant, hepatoprotective, pancreatoprotective activity in previous pharmacological study in vivo. Thus, the aim of this study was to identify and establish the fatty acid content in the plant components of antidiabetic herbal mixture. Fatty acids were separated by validated method of of gas chromatography-mass spectrometry after conversion into methyl esters. The result showed that Urtica dioica L. leaf and Vaccinium myrtillus L. leaf contain 12 fatty acids (8 saturated, 2 monounsaturated and 2 polyunsaturated), Rosa majalis L. fruits and Taraxacum officinale L. roots – 13 fatty acids (9 saturated, 2 monounsaturated and 2 polyunsaturated) and Mentha piperita L. herb – 14 fatty acids (10 saturated, 2 monounsaturated and 2 polyunsaturated). The predominant long-chain carboxylic acids in all plant raw materials were unsaturated fatty acids, their content was 55.3% in Urtica dioica L. leaf, 64.7% in Rosa majalis L. fruits, 60.5% in Vaccinium myrtillus L. leaf, 64.3% in Mentha piperita L. herb and 51.7% in Taraxacum officinale L. roots. This indicates the feasibility of including each component in the antidiabetic herbal mixture in order to form anticholesterolemic, anti-inflammatory, immunomodulatory and neuroprotective activity, due to the high content of omega-3, omega-6 and omega-9 fatty acids

Screening Study of Hypoglycemic Activity of the Herbal Mixtures (Message 1)

The aim. To study the hypoglycemic activity of the herbal mixtures, which are used in folk medicine for the prevention and treatment of diabetes mellitus type 2, but do not have a scientific basis and to establish their conditional therapeutic dose.Materials and methods. The study was performed on male albino rats weighing 180-200 g, which for preventive treatment during 20 days orally received aqueous extracts (1:10) of the studied herbal mixtures at a dose 6 mL/kg/day, 9 mL/kg/day and 12 mL/kg/day and comparison drugs – the officinal herbal mixtures “Arfazetin” at a dose 9 mL/kg/day and metformin tablets at a dose 60 mg/kg/day. The study of hypoglycemic properties and the establishment of a conditional therapeutic dose of the studied mixtures was carried out using glucose loading tests. All experiments were performed in accordance with general ethical principles with the recommendations of the EEC Council directive 2010/63/EU about the protection of animals, which are used for scientific purposes.Results. The results of the study showed that the 20-day preventive treatment by the herbal mixtures reduced alimentary hyperglycemia at the 30th minutes of OGTT and helped regulate carbohydrate tolerance disorders by reducing hyperglycemia at the 15th minutes of IPGTT. The highest hypoglycemic activity showed the herbal mixtures No. 3 (12 mL/kg/day) and No. 4 (12 mL/kg/day), which was almost on a par with the comparison drug –metformin tablets, but exceeded the officinal herbal mixture “Arfazetin”. In addition, the dose-dependence of the effectiveness of all five studied herbal mixtures was established.Conclusions. For the first time, it was conducted the screening study of hypoglycemic activity of the herbal mixtures, which are used in folk medicine for the prevention and treatment of diabetes mellitus type 2. It was determined that the greatest effectiveness in terms of the ability to reduce alimentary hyperglycemia during OGTT and reduce impaired carbohydrate tolerance during IPGTT show the herbal mixtures No. 3 (which includes Urticea folia, Cichorii radices, Rosae fructus, Elymi repens rhizomata, Taraxaci radices) and No. 4 (which includes Arctii lappae radices, Elymi repens rhizomata, Maydis style cum sigmatis, Helichrysi arenarii flores, Rosae fructus). It was established their conditional therapeutic dose of 12 mL/kg/da

HPLC-DAD assay of phenols profile in Antennaria dioica (L.) Gaertn

Antennaria dioica (Asteraceae family) – is a perennial herb, commonly found in dry grasslands and sandy or stony places from Eurasian areas. It is known in traditional medicine as antioxidant, diuretic, choleretic and anti-inflammatory remedy. This species should be reconsidered as possible sources of phenols, mainly flavonoids and hydroxycinnamic acids. Thus, the aim of this study was to validate a chromatographic method for detection of phenols and their identification in A. dioica herb. HPLC-DAD method was evaluated in terms of linearity, precision, repeatability, accuracy, LOD and LOQ. The calibration curves of thirteen reference substances were linear (R2 > 0.99) over the range of 5–400 µg/mL, the LODs and the LOQs were in the range of 0.1–0.3 µg/mL and 0.2–1.0 µg/mL, respectively. During HPLC-DAD assay two flavones – luteolin, apigenin; flavonol – quercetin and three its glycosides – rutin, hyperoside and isoquercitrin; coumarins: coumarin and umbelliferone; five hydroxycinnamic acids – chlorogenic, caffeic, p-coumaric, trans-ferulic and rosmarinic were identified in A. dioica herb. This phytochemical study of A. dioica confirms that this plant material is a rich source of phenolic compounds

Determination of Carboxylic Acids Content in the Herbal Mixtures by HPLC

The aim of the study was to research the qualitative composition and to investigate the quantitative content of some carboxylic acids in the herbal mixtures with established hypoglycemic, hypolipidemic and antioxidant activity in previous studies in vivo. Materials and methods. Studies of carboxylic acid content in the herbal mixtures were performed by HPLC analysis using Agilent Technologies 1200 liquid chromatograph (USA). Identification and quantitative analysis were performed using standard solutions of carboxylic compounds (tartaric, pyruvic, isocitric, citric, succinic and fumaric acids). Conclusions. HPLC analysis of five samples of the herbal mixture with antidiabetic activity showed the presence of six carboxylic acids. The dominant acid in all samples was isocitric acid. Among the most important for the prevention and treatment of diabetes, high levels of succinic and fumaric acids have been identified and established. The obtained data indicate a correlation between the phytochemical composition of the studied herbal mixtures and their pharmacodynamics, which was previously establishe