A fluorometric method for the assay of protein kinase activity

Protein kinases constitute one of the largest protein families in nature. Current methods to assay their activity involve the use of radioactive ATP or very expensive reagents. In this work, we developed a highly sensitive, cost-effective and straightforward protocol to measure protein kinase activity using a microplate layout. Released ADP is converted into NAD+, which is quantified by its fluorescent properties after alkaline treatment (linear range 0–10 nmol ADP). To validate our protocol, we characterized a recombinant calcium-dependent protein kinase from potato. Overall, this tool represents a critical step forward in the functional characterization of protein kinases.Fil: Rojas, Bruno Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Santin, Franco. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Ulloa, Rita Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; ArgentinaFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentin

Analysis of drugs of abuse metabolites using passive sampling and ultrahigh-liquid chromatography coupled to mass spectrometry

El presente estudio propone la monitorización de metabolitos de drogas de abuso mediante el uso de muestreadores pasivos en sistemas de agua. Inicialmente se determinaron cuatro metabolitos iónicos positivos de interés según las encuestas nacionales, luego se implementó el muestreo compuesto y el muestreo pasivo utilizando el equipo de muestreo pasivo de flujo continuo con dos tipos de sorbentes en su interior, Empore Disk y Gerstel Twister. Se determinaron dos sitios de estudio al inicio y al final de la cuenca media del río Bogotá, después de 4 días se retiraron los sorbentes para que fueran desorbidos y analizados con la metodología UHPLC-MS en laboratorio. Para los muestreos compuestos los resultados estuvieron por debajo del FCCP del método cromatográfico y para los muestreos pasivos se encontraron picos de benzoilecgonina (21427,3 pg mL-1), metanfetamina (67101,5 pg mL-1), éxtasis (225844,8 pg mL-1) y metadona (15908,4 pg mL-1), lo que permite postular el muestreo pasivo como una alternativa al muestreo compuesto en el monitoreo de metabolitos.The present study proposes the monitoring of metabolites of drugs of abuse through the use of passive samplers in water systems. Initially, four positive ion metabolites of interest were determined according to national surveys, then composite sampling and passive sampling were implemented using the continuous flow passive sampler equipment with two types of sorbents inside, Empore Disk and Gerstel Twister. Two study sites were determined at the beginning and at the end of the middle Bogotá River basin after 4 days the sorbents were removed so that they could be desorbed and analyzed using UHPLC-MS methodology in laboratory. For composite samplings, results were below the FCCP of the chromatographical method and for passive sampling, peaks of benzoylecgonine (21427.3 pg mL-1), methamphetamine (67101.5 pg mL-1), ecstasy (225844.8 pg mL-1) and methadone (15908.4 pg mL-1) were found, allowing the postulation of passive sampling as an alternative to composite sampling in the monitoring of metabolites.Magíster en HidrosistemasMaestríahttps://orcid.org/0000-0002-1846-0406https://scienti.minciencias.gov.co/cvlac/visualizador/generarCurriculoCv.do?cod_rh=0001725391Se hace necesario un año de espera puesto que el documento va a ser enviado para proceso evaluativo y posterior posible publicación en revista internacional titulada Journal of Environmental Science and Health, Part A de Taylor & Francis y la revisión puede durar entre 8 a 12 meses aproximadamente

Implementation of a modified Graham-Walles viscosity function within a Chaboche viscoplastic constitutive model

peer reviewedThis work provides a numerical framework for the accurate prediction of operational life of metallic components exhibiting a non-classical creep behavior under constant loadings and very high temperature. A modified Graham-Walles type analytical viscoplastic function is implemented into a Chaboche unified viscoplastic constitutive model. The numerical model is integrated into the finite element software Lagamine following a fully-implicit two-step radial return mapping algorithm. The non-linear system of equations is solved using a robust Newton-Raphson method. The computational efficiency of the model is enhanced by implementing a sub-step routine, thereby decreasing the average number of iterations of the finite element software. The validation of the model is performed using experimental data available in the literature on the non-classical creep behavior of Incoloy 800H, a Ni-superalloy exhibiting a two-step creep strain rate minima attributed to multiple complex dislocation-precipitate interactions.Development of a generic MultiScale Creep-Fatigue approach, allowing finite element simulations to predict strains and fracture of metal components at high temperature- application on 800H alloy9. Industry, innovation and infrastructur

Concordancia y confiabilidad de una prueba neuromuscular y cognitiva basada en estímulos de luz para evaluar el tiempo de reacción integrativo en el deporte

La reacción en los deportes de equipo implica una serie de procesos sistémicos que integran funciones para desarrollar una respuesta considerando la situación, el tiempo y el espacio. El objetivo de este estudio fue analizar la concordancia y la confiabilidad de una prueba neuromuscular y cognitiva basada en estímulos de luz para evaluar el tiempo de reacción integrativo en los deportes. Catorce participantes activos realizaron dos sesiones con 10 repeticiones de la Prueba de Reacción Deportiva NeuroPhys (NPSRT). La prueba mostró confiabilidad moderada a casi perfecta (coeficiente de correlación intraclase = 0.53-0.81) y buena concordancia (Método Bland-Altman, BIAS = -0.0004-0.029) entre sesiones; no hubo diferencias medias absolutas entre sesiones (prueba t de medidas repetidas = p≤0.050) y presentaron correlaciones lineales moderadas a altas (Pearson r = 0.53-0.83, p≤0.05). El NPSRT podría considerarse como una prueba confiable para evaluar el tiempo de reacción en los deportes de equipo.Reaction in team sports involves a series of systemic processes integrating functions to develop a response considering situation, time and space. The aim of this study was to analyze the agreement and reliability of a neuromuscular and cognitive test based on light stimuli to assess integrative reaction time in sports. Fourteen active participants performed two sessions with 10 repetitions of the NeuroPhys Sport Reaction Test (NPSRT). The test showed moderate to almost perfect reliability (intraclass correlation coefficient = 0.53-0.81) and good agreement (Bland-Altman Method, BIAS = -0.0004-0.029) between sessions; there were no absolute mean differences between sessions (repeated measures t-test = p≤0.050) and presented moderate to high lineal correlations (Pearson r = 0.53-0.83, p≤0.05). The NPSRT could be considered as a reliable test to assess reaction time in team sports.peerReviewe

Efficient thermo-mechanical modelling of cyclic loading with Chaboche type constitutive law coupled with damage

peer reviewedThis paper describes a numerical modelling approach to study the behaviour of a solar receiver tube subjected to cyclic thermomechanical loading. The Lagamine finite element (FE) code was utilized along with a Chaboche type material law and a Lemaitre's unified damage model to simulate the material behaviour under fatigue, creep, and corrosion. The cycle jump procedure is evaluated, as it is a method for efficient computation of long-term evolution of material behaviour under cyclic loading. The procedure involves computing a number of cycles in the FE code, then extrapolating the results from these cycles over a number of cycles. This alternating process is repeated until the end of the computation. A parametric study was achieved to assess the effects of different strategies within the cycle jump. It was observed that the strong evolution of the material behaviour for the first cycles (around 100 cycles) of the computation prevented the use of the cycle jump during that period. Also, a sufficient number of cycles (minimum 4) must be computed with the FE code between the jumps to ensure reaching a stable solution. With the optimum parameters, the cycle jump permitted to significantly decrease the computation time (factor 10), while having a limited impact on the accuracy of the results (lower than 1%).Solar GNext7. Affordable and clean energy9. Industry, innovation and infrastructure13. Climate actio

Assessment of the influence of creep transition and nitridation in the creep-life prediction of Incoloy 800H

Accurate prediction of creep life of materials has been for years a matter of high research interest. The engineering design of such components is often performed following standardized analytical procedures aimed to empirically correlate state variables (mainly stress and temperature) with the chosen failure criteria (e.g., buckling, time-to-1% strain, time-to-rupture, etc). However, the accuracy of the chosen model ultimately depends on the microstructural properties of the material. As such, changes in thermomechanical treatments and environmental conditions can largely affect the creep behaviour of such components, thus making inadequate the use of simplified analytical models (R. W. Swindeman and D. L. Marriot, “Criteria for design with structural materials in combined-cycle applications above 815°F”, in Journal of Engineering for Gas Turbines and Power, vol. 116, pp. 352-359, 1993). Such is the case of Incoloy 800H, a solution-annealed austenitic Fe-Ni-Cr alloy of high industrial interest as it provides a good balance between production cost and high-temperature mechanical response. Under particularly low-stress and high-temperature loadings, this alloy is reported to exhibit a diffusion-to-dislocation transitional creep. Furthermore, the subsequent large dislocation-driven tertiary creep stage undergoes a nitridation-induced hardening while exposed to high-N environments (V. Guttmann and R. Bürgel, “Creep-structural relationship in steel alloy 800H at 900-1000°C”, in Metal Science, vol. 17, pp. 549-555, 1983). In this work, the creep behaviour of the alloy is modelled using a Chaboche-type constitutive law (H. Morch et al., “Efficient temperature dependence of parameters for thermo-mechanical finite element modelling of alloy 230”, in European Journal of Mechanics / A Solids, vol.85, 2020) implemented in the MSM-team (ULiège) proprietary finite element software Lagamine. The results are later assessed with the aim of proposing a novel and efficient numerical creep micromechanics approach intended for the identification of Chaboche parameters while addressing the underlaying uncertainties that rule the creep behaviour of this alloy: diffusion-dislocation creep transition and nitridation.Development of a generic MultiScale Creep-Fatigue approach, allowing finite element simulations to predict strains and fracture of metal components at high temperature-application on two Ni-Cr alloysMSCreep9. Industry, innovation and infrastructur

Transgresiones de derechos ambientales y la quema de saccharum officinarum Laredo – Perú

" Violations of environmental rights and the burning of saccharum officinarum Laredo Peru" is the cientific article whose objective was to diagnose the correlation between the violation of environmental rights and the state's duty in the face of environmental impacts. existing negatives due to the burning of sugar cane in Laredo, 2022. The research is quantitative, basic and descriptive; cross-sectional correlational design. The sample population was made up of 50 experts; The survey was used to collect data with two questionnaires, validated by expert judgment. These results allowed us to conclude that there is a low and non-significant correlation between both variables; Therefore, an environmental health proposal is urgently needed to reduce the damage caused by the burning of cane in Laredo, by the environmental authorities or entrepreneurs of the Laredo sugar agribusiness.“Transgresiones de derechos ambientales y la quema de saccharum officinarum Laredo Perú” es el artículo cientifico cuyo objetivo fue diagnosticar la correlación entre la transgresión de los derechos ambientales y el deber del Estado ante impactos ambientales negativos existentes por la quema de caña de azúcar en Laredo, 2022. La investigación es cuantitativa, básica y descriptiva; de diseño correlacional transversal. La población muestral se conformó con 50 expertos; se empleó la encuesta para recolectar datos con dos cuestionarios, validados por el juicio de expertos. Estos resultados permitieron arribar a la conclusión que, entre ambas variables existe una correlación baja y no significativa; por lo que urge una propuesta de salud ambiental para disminuir el daño que causa la quema de caña en Laredo, por parte de las autoridades ambientales o empresarios de la agroindustria azucarera Laredo

Abuso de prisión preventiva y hacinamiento carcelario durante crisis sanitaria por covid-19 en penal de varones de Trujillo 2021

The objective of the investigative work is to explain the abuse of preventive detention during the health emergency due to COVID-19 in the overcrowding of the El Milagro -Trujillo prison, 2021. For this, the qualitative approach has been used, which collects and analyzes non-numerical data to understand concepts, opinions or experiences of the professionals interviewed that reveal emotions or behaviors necessary for the preparation of this document. In addition, the phenomenological design. For the processing of the information obtained from the responses of our interviewees, it has been subjected to a rigorous analysis in order to subject them to discussion and debate. The most important conclusions were that the release of pre-trial prisoners during the health crisis due to COVID-19 has been recognized due to the use of D.Leg. 1513, consequently, a slight decrease in preventive inmates in 2020. However, in 2021 the number of preventive inmates increased, so much so that to date they represent almost 35 percent of the total population. Which reveals that the D. Leg. The purpose of which is to release inmates to prevent the spread of COVID-19 has been circumstantial, however, the underlying problem has not been solved: prison overcrowding.El trabajo investigativo tiene como objetivo explicar el abuso de la prisión preventiva durante la emergencia sanitaria por COVID-19 en el hacinamiento del penal El Milagro -Trujillo, 2021. Para ello se ha empleado el enfoque cualitativo el cual recoge y analiza datos no numéricos para comprender conceptos, opiniones o experiencias de los profesionales entrevistados que revelan emociones o comportamientos necesarios para la elaboración del presente documento. Además, el diseño fenomenológico. Para el procesamiento de la información obtenida de las respuestas de nuestros entrevistados ha sido sometida a un riguroso análisis con el fin de someterlas a discusión y debate. Las conclusiones más importantes fueron que se ha reconocido la excarcelación de los presos preventivos durante la crisis sanitaria por COVID-19 debido al empleo del D.Leg. 1513, en consecuencia, una leve disminución de reos `preventivos en el 2020. Sin embargo, el 2021 el número de internos preventivos aumentó, tan es así que a la fecha representan casi el 35 por ciento de la población total. Lo que revela que el D. Leg. que tiene por finalidad la excarcelación de los reclusos para evitar el contagio de la COVID-19 ha sido coyuntural, no obstante, no se ha solucionado el problema de fondo: el hacinamiento penitenciario.&nbsp

Identification of a soft matrix-hard inclusion material by indentation

peer reviewedA new procedure for identifying the mechanical behavior of individual phases within a bi-material (matrix-particles) is presented. The case of AlSi10Mg (large globularized Si-rich particles surrounded by an α-Al phase) processed by additive manufacturing and post-treated is taken as a typical example. Grids of nano-indentation tests are performed at different locations on the nanocomposite using a Berkovich indenter and show an impact of the hard inclusions on the experimental curves. The elastoplastic properties of the matrix are identified based on the lowest load–indentation depth curves. Several representative finite element (FE) models demonstrate the influence of the particles on the nano-indentation response. The capacity of the FE model to predict the indentation curve of a cube corner indenter experiment and the Berkovich grid result scattering was checked. A representative volume element (RVE) based on a scanning electron microscope (SEM) image is defined. The identified material parameters of the α-Al phase and Si phase, it allows the prediction of the stress-strain curve of a macroscopic experimental tensile test.LongLifeA

Electromagnetic Evidence that SSS17a is the Result of a Binary Neutron Star Merger

11 hours after the detection of gravitational wave source GW170817 by the Laser Interferometer Gravitational-Wave Observatory and Virgo Interferometers, an associated optical transient SSS17a was discovered in the galaxy NGC 4993. While the gravitational wave data indicate GW170817 is consistent with the merger of two compact objects, the electromagnetic observations provide independent constraints of the nature of that system. Here we synthesize all optical and near-infrared photometry and spectroscopy of SSS17a collected by the One-Meter Two-Hemisphere collaboration. We find that SSS17a is unlike other known transients. The source is best described by theoretical models of a kilonova consisting of radioactive elements produced by rapid neutron capture (the r-process). We find that SSS17a was the result of a binary neutron star merger, reinforcing the gravitational wave result.Comment: 21 pages, 4 figures, accepted to Scienc