Documentación e información para mayores: actividades formación en competencias informacionales en el Centro de Mayores de Santa Engracia (Madrid)

Memoria final de un proyecto con alumnos del Grado en Información y Documentación y dos Centros de Mayores del Ayuntamiento de Madrid

Functional characterization of rs2229094 (T>C) polymorphism in the tumor necrosis factor locus and lymphotoxin alpha expression in human retina: the Retina 4 project

Abstract Purpose: The objective of this study is to determine the expression and localization of lymphotoxin alpha (LTA) in human retinas and the functionality of one of its polymorphisms rs2229094 (C13R) (T>C), previously associated with proliferative vitreoretinopathy (PVR) development. Materials and methods: Total RNA from three healthy human retinas were extracted and subjected to reverse transcription-polymerase chain reaction (RT-PCR) analysis, using flanking primers of LTA cDNA. In addition, three human eyes with retinal detachment (RD) and three healthy control eyes were subjected to immunohistochemistry (IHC) with a specific antibody against LTA. The functionality of T and C alleles was assessed by using pCEFL-Flag expression vector and transient transfection assays in COS-1 cell line. In addition, expression analysis by RT-PCR, Western blot and subcellular localization of both alleles and by immunofluorescence assay was performed. Results: RT-PCR analysis revealed no significant levels of messenger RNA (mRNA) LTA in healthy human retinas. Sequential IHC staining showed differences between healthy human and RD retinas. No differences in mRNA and protein expression levels and in subcellular localization between both alleles were found. Both alleles were located in the cytoplasm of COS-1 cells. Conclusion: Although results suggest lack of functionality, the differences found in IHC study and its strong association with PVR and its relationship with tumor necrosis factor locus, warrant further studies and could justify the use of this polymorphism as a valid biomarker to identify high-risk patients to develop PVR after RD. Keywords: cytokines; inflammation; lymphotoxin alpha; polymorphism; proliferative vitreoretinopathy; tumor necrosis factor alpha

RIED. Revista iberoamericana de educación a distancia

Resumen basado en el de la publicaciónLa incursión de las nuevas tecnologías en entornos educativos, especialmente en aquellos de educación a distancia ha supuesto una auténtica revolución. El Campus Virtual es por definición el conjunto de funcionalidades que hacen posible la interacción entre los colectivos que componen la universidad, sin necesidad de coincidir ni en el espacio ni en el tiempo. Se presenta el proyecto Campus Virtual, continuación del proyecto DEMOS que fue iniciativa de la UNED (Universidad Nacional de Educación a Distancia), y que supone un entorno educacional donde la cooperación entre profesores, alumnos, tutores y expertos se realiza de modo flexible a través de la utilización de herramientas multimedia.MadridES