Sex Determination by Evaluating Inter-Canine Distance and Mesio-Distal Width of Mandibular Canine

INTRODUCTION: Establishing one’s identity is necessary for legal as well as humanitarian purpose. Sex determining is considered to be one of the major factors employed to assist for the identification of a person. Individual identification heavily relies on the quality of the dental records in the office.AIM: The purpose of this study was to assess the usefulness of the Mandibular Canine Index(MCI) as an aid in sexual dimorphism in Kanpur population using mesiodistal with intercanine distance of mandibular canines.MATERIALS AND METHOD: This was an experimental study involving the preparation of plaster casts of 200 males and females each residing in Kanpur, UP, India. Both the genders were in the age group of 17-30 years. The casts for study were prepared and measurements of mandibular canine along with intercanine distance were taken. The MCI calculations were done using standard equations. Statistical analysis used students’t-test. All values were considered statistically significant for value of p<0.05.RESULTS: The present study demonstrated that left MCI predictability for males is more whereas in case of females, right MCI predictability is more, with no significant variation in the intercanine distance.CONCLUSION: It was found out that mandibular canines exhibit maximum sexual dimorphism, hence, they can be considered as the key teeth for one’s identification

An unusual anomaly of deep venous system in the lower limb: Complete unilateral agenesis of iliofemoral veins in the absence of persistent sciatic vein

Venous anomalies are the most common congenital vascular anomalies. The rarest of these is complete deep venous agenesis. Here,we report a case of a 15-year-old male patient evaluated on color Doppler and computed tomographic venogram with complete rightiliofemoral deep venous agenesis with the inferior vena cava continuing as the left common iliac vein and the right popliteal veindraining into superficial anomalous veins of the thigh. These superficial veins were seen to drain through a superficial suprapubicarcuate connector vein into the dilated left common femoral vein. Uniquely in our case, there was no persistent embryonic sciaticvein and no demonstrable reflux into the superficial connector vein, suggesting an uncommon embryological mechanism underlyingits pathogenesis. Surgical resection of the dilated superficial veins is absolutely contraindicated in cases of deep venous agenesis andhence accurate diagnosis by the radiologist is absolutely essential in such cases

Structure and expression analysis of early auxin-responsive Aux/IAA gene family in rice (Oryza sativa)

Auxin exerts pleiotropic effects on plant growth and development by regulating the expression of early auxin-responsive genes of auxin/indoleacetic acid (Aux/IAA), small auxin-up RNA, and GH3 classes. These genes have been studied extensively in dicots like soybean and Arabidopsis. We had earlier characterized a cDNA of the first monocot member of Aux/IAA family from rice. The achievement of the large scale rice genome sequencing combined with the availability of full-length cDNA sequences from Knowledge-based Oryza Molecular Biological Encyclopedia provided us the opportunity to draw up the first comprehensive list of Aux/IAA genes in a monocot. By screening the available databases, we have identified 31 Aux/IAA genes having high sequence identity within the conserved domains I, II, III, and IV. The genomic organization as well as chromosomal location of all the Oryza sativa indoleacetic acid (OsIAA) genes is reported. The rice Aux/IAA proteins can be classified in two groups (A and B) on the basis of their phylogenetic relationship with Arabidopsis Aux/IAA proteins. An evolutionary pattern of the rice Aux/IAA genes has been discussed by analyzing their structure (exon/intron organization) and duplications. Interestingly, the duplication of rice Aux/IAA genes was found to be associated with chromosomal block duplication events in rice. The in-silico analysis has been complemented with real-time polymerase chain reaction analysis to quantify transcript levels of all Aux/IAA family members. OsIAA genes showed differential and overlapping organ-specific expression patterns in light- and dark-grown seedlings/plants. Although auxin enhanced the transcript abundance of most of the OsIAA genes, the effect was more pronounced on OsIAA9, 14, 19, 20, 24, and 31. These results provide a foundation for future studies on elucidating the precise role of rice Aux/IAA genes in early steps of auxin signal transduction

Unravelling Differential DNA Methylation Patterns in Genotype Dependent Manner under Salinity Stress Response in Chickpea

DNA methylation is one of the epigenetic mechanisms that govern gene regulation in response to abiotic stress in plants. Here, we analyzed the role of epigenetic variations by exploring global DNA methylation and integrating it with differential gene expression in response to salinity stress in tolerant and sensitive chickpea genotypes. Genome-wide DNA methylation profiles showed higher CG methylation in the gene body regions and higher CHH methylation in the TE body regions. The analysis of differentially methylated regions (DMRs) suggested more hyper-methylation in response to stress in the tolerant genotype compared to the sensitive genotype. We observed higher enrichment of CG DMRs in genes and CHH DMRs in transposable elements (TEs). A positive correlation of gene expression with CG gene body methylation was observed. The enrichment analysis of DMR-associated differentially expressed genes revealed they are involved in biological processes, such as lateral root development, transmembrane transporter activity, GTPase activity, and regulation of gene expression. Further, a high correlation of CG methylation with CHG and CHH methylation under salinity stress was revealed, suggesting crosstalk among the methylation contexts. Further, we observed small RNA-mediated CHH hypermethylation in TEs. Overall, the interplay between DNA methylation, small RNAs, and gene expression provides new insights into the regulatory mechanism underlying salinity stress response in chickpeas

Effect of mode of rolling on development of texture and microstructure in two-phase (alpha plus beta) brass

The evolution of microstructure and texture during deformation of two-phase (alpha + beta) brass was studied for different initial microstructure and texture. The deformation processing involved unidirectional and multi-step cross-rolling. The bulk textures were determined by measuring the pole figures and calculating the orientation distribution function ODF for both alpha (fcc) and beta (bcc) phases, while the microstructure and other microstructural parameters were measured through optical microscopy and scanning electron microscopy with electron back scatter diffraction (SEM-EBSD). Results indicate that textures developed after unidirectional rolling and multi-step cross-rolling are significantly different. The variation in initial texture had a pronounced effect on the development of texture in the alpha phase during subsequent deformation. (C) 2010 Elsevier B.V. All rights reserved

Abiotic Stress: Molecular Genetics and Genomics

Abiotic stresses are the major cause that limits productivity of crop plants worldwide. Plants have developed intricate machinery to respond and adapt over these adverse environmental conditions both at physiological and molecular levels. Due to increasing problems of abiotic stresses, plant biotechnologists and breeders need to employ new approaches to improve abiotic stress tolerance in crop plants. Although current research has divulged several key genes, gene regulatory networks and quantitative trait loci that mediate plant responses to various abiotic stresses, the comprehensive understanding of this complex trait is still not available. This e-book is focused on molecular genetics and genomics approaches to understand the plant response/adaptation to various abiotic stresses. It includes different types of articles (original research, method, opinion and review) that provide current insights into different aspects of plant responses and adaptation to abiotic stresses

Complex structural phase transitions in slightly Ca modified Na0.5Bi0.5TiO3

A temperature dependent neutron powder diffraction study, in conjunction with dielectric and ferroelectric characterization, of slightly Ca modified Na0.5Bi0.5TiO3 (NBT) revealed an instability with regard to a non-polar orthorhombic (Pbnm) distortion above room temperature. This intermediate orthorhombic phase has earlier been reported for unmodified NBT by electron diffraction studies, but has never been captured by global (x-ray/neutron) diffraction techniques. Calcium substitution seems to amplify the magnitude of this intermediate orthorhombic distortion thereby making the corresponding superlattice reflections become visible in the neutron diffraction pattern. The study revealed the following sequence of very complex structural evolution with temperature: Cc -> Cc + Pbnm -> Pbnm + P4/mbm -> P4/mbm -> Pm (3) over barm