Isolasi dan identifikasi bakteri selulolitik dari sampah organik sayuran dan buah-buahan yang berperan dalam pembuatan biogas

Sampah mengandung bermacam-macam mikroba, meliputi berbagai spesies bakteri, cendawan dan lain-lain. Salah satu sumber sampah terbanyak adalah sisa buah-buahan dan sayur-sayuran yang banyak terdapat di pasar-pasar tradisional. Sampah organik sayuran dan buah-buahan ini tersusun atas bahan organik dan serat tinggi seperti selulosa. Bahan-bahan organik yang mengandung serat tinggi tersebut merupakan sumber bakteri selulolitik yang berperan dalam penyediaan prekursor biogas. Penelitian ini bertujuan untuk mengetahui bakteri apa saja yang tumbuh pada sampah organik sayuran dan buah-buahan dan mengetahui isolat bakteri selulolitik apa saja yang berperan dalam pembuatan biogas. Penelitian menggunakan metode eksperimen analisis deskriptif. Media yang digunakan untuk membiakan, dan mengisolasi bakteri adalah medium NA (Natrium Agar). Isolasi bakteri dilakukan dengan menggunakan metode pengenceran cawan tuang dan cawan gores. Untuk mengetahui bakteri selulolitik menggunakan medium selektif SA (Selulosa Agar) dan Identifikasi bakteri dilakukan sampai tingkat genus berdasarkan kepada karakterisasi morfologi dan biokimia yang mengacu kepada pedoman identifikasi bakteri (Bergey’s Manual Determinative Bacteriology tahun 1994). Pada penelitian ini diperoleh 13 isolat yang cenderung masuk ke 10 Genus yaitu Bacillus, Azomonas, Cellulomonas, Microbacterium, Neisseria, Streptomycetes, Streptococcus, Halobacterium, Listeria dan Arthrobacter. Dari uji selulosa maka diketahui bahwa bakteri selulolitik adalah Bacillus, Cellulomonas, Microbacterium, Streptomycetes, Streptococcus, dan Neisseria. Berdasarkan uji HC dalam degradasi selulosa pada medium SA, Streptomycetes adalah bakteri yang memiliki ratio HC tertinggi dan yang terendah pada Genus Neisseria. Bakteri tersebut dipilih karena bakteri mempunyai kemampuan tinggi dalam menghidrolisis selulosa sehingga dinamakan bakteri selulolitik dan berpotensi dalam pembuatan biogas

ISOLATION AND IDENTIFICATION OF CELLULOLYTIC BACTERIA FROM WASTE ORGANIC VEGETABLES AND FRUITS FOR ROLE IN MAKING MATERIALS BIOGAS.

Research onisolationandidentification ofcellulolyticbacteriafromwasteorganicvegetablesand fruitsas ingredientsthat play a rolein the manufacture ofbiogashas been carried out. Bacteria isolates were obtained from wastevegetableand fruitsfromthe marketGedebageBandung. The aim of research is to know diversity of bacteria that growandcellulolyticbacterialisolateswhatever ison thewastevegetablesand fruitsthat play a rolein makingbiogas. Media that used for cultivation and isolation bacteria is NA (Natrium Agar). Bacteria isolation is conducted by using serial dilution, pour plate and streak plate methods. The determinecellulolyticbacteriausedselective mediaSA(Cellulose Agar) andidentification ofbacteriacarriedto genus levelbased onmorphologicalandbiochemicalcharacterizationthat refersto theguidelinesof identificationof bacteria (Bergey's Manual ofDeterminativeBacteriologyin 1994). In this research, we successfully isolated 13 isolates bacteria that consist of 10 genera. Identification result indicates that isolate B1, B8, B9, and B12 are Bacillus; isolate B2 is Cellulomonas, isolate B4 is Microbacterium, isolate B5 is Neisseria, isolate B6 is Streptococcus and isolate B7 is Strepmycetes. Test cellulose indicates that the cellulolyticbacteriaareBacillus, Cellulomonas, Microbacterium, Streptomycetes, Streptococcus, andNeisseria. Based ontestinHCdegradation ofcellulosein the mediaSA, Streptomycetesis bacteria thathave thehighestHCratioandthelowestin thegenusNeisseria. The bacteriawere chosen becausethe bacteriahave ahighability tohydrolyzecelluloseinso-called cellulolyticbacteriaand potentiallyin the manufacture ofbiogas

ISOLATION AND IDENTIFICATION OF CELLULOLYTIC BACTERIA FROM WASTE ORGANIC VEGETABLES AND FRUITS FOR ROLE IN MAKING MATERIALS BIOGAS.

Research onisolationandidentification ofcellulolyticbacteriafromwasteorganicvegetablesand fruitsas ingredientsthat play a rolein the manufacture ofbiogashas been carried out. Bacteria isolates were obtained from wastevegetableand fruitsfromthe marketGedebageBandung. The aim of research is to know diversity of bacteria that growandcellulolyticbacterialisolateswhatever ison thewastevegetablesand fruitsthat play a rolein makingbiogas. Media that used for cultivation and isolation bacteria is NA (Natrium Agar). Bacteria isolation is conducted by using serial dilution, pour plate and streak plate methods. The determinecellulolyticbacteriausedselective mediaSA(Cellulose Agar) andidentification ofbacteriacarriedto genus levelbased onmorphologicalandbiochemicalcharacterizationthat refersto theguidelinesof identificationof bacteria (Bergey's Manual ofDeterminativeBacteriologyin 1994). In this research, we successfully isolated 13 isolates bacteria that consist of 10 genera. Identification result indicates that isolate B1, B8, B9, and B12 are Bacillus; isolate B2 is Cellulomonas, isolate B4 is Microbacterium, isolate B5 is Neisseria, isolate B6 is Streptococcus and isolate B7 is Strepmycetes. Test cellulose indicates that the cellulolyticbacteriaareBacillus, Cellulomonas, Microbacterium, Streptomycetes, Streptococcus, andNeisseria. Based ontestinHCdegradation ofcellulosein the mediaSA, Streptomycetesis bacteria thathave thehighestHCratioandthelowestin thegenusNeisseria. The bacteriawere chosen becausethe bacteriahave ahighability tohydrolyzecelluloseinso-called cellulolyticbacteriaand potentiallyin the manufacture ofbiogas

Isolation and identification of cellulolytic bacteria from Waste organic vegetables and fruits for role in making Materials biogas.

Research on isolation and identificationof cellulolytic bacteria from waste organic vegetables and fruitsas ingredients that play a rolein the manufacture of biogas has been carried out. Bacteria isolates were obtained from waste vegetable and fruits from the market Gedebage Bandung. The aim of research is to know diversity of bacteria that grow and cellulolytic bacterial isolates whatever ison the waste vegetables and fruit sthat play a rolein making biogas. Media that used for cultivation and isolation bacteria is NA (Natrium Agar). Bacteria isolation is conducted by using serial dilution, pour plate and streak plate methods. The determine cellulolytic bacteria used selective media SA(Cellulose Agar) and identification ofbacteriacarriedto genus level based on morphological and biochemical characterization that refersto the guidelines of identification of bacteria (Bergey's Manual of Determinative Bacteriology in 1994). In this research, we successfully isolated 13 isolates bacteria that consist of 10 genera. Identification result indicates that isolate B1, B8, B9, and B12 are Bacillus; isolate B2 is Cellulomonas, isolate B4 is Microbacterium, isolate B5 is Neisseria, isolate B6 is Streptococcus and isolate B7 is Strepmycetes. Test cellulose indicates that the cellulolytic bacteria are Bacillus, Cellulomonas, Microbacterium, Streptomycetes, Streptococcus, and Neisseria. Based on test in HC degradation of cellulose in the mediaSA, Streptomycetesis bacteria that have the highest HC ratio and the lowest in the genus Neisseria. The bacteria were chosen because the bacteria have a high ability to hydrolyze cellulose inso called cellulolytic bacteria and potentiallyin the manufacture of biogas