Protein Array Fabrication with E-jet Printing

We demonstrate the feasibility of fabricate biosensors with direct protein printing without complicated processes at high resolution and area coverage. Printed single protein, streptavidin-fluorescein, binds to biotin labeled with fluorescent DNA without denaturation; therefore a fluorescence microscope can detect the printed droplets as shown in Figure 7.1(a). The standard deviation of the spot size and spacing is 7.26 % and 0.693%, respectively. The e-jet printing is capable to print large area, e.g., one inch size. Half droplets of streptavidin-Cy5 are printed in 15 minutes (Figure 7.1(b)). Four-inch size printing is also available. Moreover, we have developed the multi-syringe head [1]. The head prints multiple inks by rotation. Even complicated patterns of multiple proteins (e.g., peacock shown in Figure 7.1(c)) can be printed. Printed primary antibodies can be detected under a fluorescence microscopy by incubating with fluorescently-labeled secondary antibodies [2]. These capabilities show that the e-jet printer with the multi-syringe head has feasibility to make protein chips to detect multiple proteins at lower cost than the conventional chips

Alien Registration- Rogers, John A. (Rumford, Oxford County)

https://digitalmaine.com/alien_docs/13552/thumbnail.jp

Cyanide practice

The ENCINO CYANIDE PLANT was erected for the treatment of old accumulated tailings, which were worked by the Patio and Cazo processes. As with most accumulated tailings, which have been exposed to the action of the air for a considerable period, they have undergone changes, whereby, forming compounds which are highly detrimental to a solution of Potassium Cyanide and the direct cause of the present high consumption of Cyanide --page 1

A Biblical-Theological Framework for Human Sexuality: Applications to Private Sexuality

What are good sexual acts? It is not that surprising when cultural voices, without reference to God, argue for the inherent goodness of all “unharmful” sexual desires and acts. Regrettably, ethical pragmatism has influenced some Christian sexual ethics, and this influence is particularly evident with the issue of masturbation. What God defines as good sexual acts are those that fulfill his unitive and procreative purposes for sex within marriage. Given God’s unitive and procreative purposes for sex within the context of marriage, we argue that masturbation is a categorically impermissible act because it fulfills neither of these purposes, and we counter Christian arguments for its permissibility. God calls Christians to deal with sexual desires, including good sexual desires, through either marital sexual expression or Spirit-enabled self-control

Protein synthesis in skeletal muscle following acute exhaustive exercise

Cell-free and whole-tissue protein synthesis was studied in skeletal muscle of untrained male guinea pigs that had undergone a treadmill run to exhaustion. Experiments using explants from the gastrocnemius muscle maintained in organ culture demonstrated that the ability of the acutely exercised muscle to incorporate amino acids into protein had increased. Compared to polyribosomes prepared from several lower hind limb muscles of nonexercised guinea pigs, polyribosomes from the same muscle in exhausted guinea pigs had incorporated almost 50% more radioactive leucine into protein. However, the polysome profiles of control and exercised muscle were identical, and no difference in the total polysome RNA content could be detected. The efficiency of in-vitro protein synthesis using washed membrane-bound polyribosomes (microsomes) isolated from acutely exercised skeletal muscle was 50% greater than with microsomes from rested control muscle.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/50128/1/880020403_ftp.pd

Emergent decision-making in biological signal transduction networks

The complexity of biochemical intracellular signal transduction networks has led to speculation that the high degree of interconnectivity that exists in these networks transforms them into an information processing network. To test this hypothesis directly, a large scale model was created with the logical mechanism of each node described completely to allow simulation and dynamical analysis. Exposing the network to tens of thousands of random combinations of inputs and analyzing the combined dynamics of multiple outputs revealed a robust system capable of clustering widely varying input combinations into equivalence classes of biologically relevant cellular responses. This capability was nontrivial in that the network performed sharp, nonfuzzy classifications even in the face of added noise, a hallmark of real-world decision-making

Boolean Modeling of Biochemical Networks

The use of modeling to observe and analyze the mechanisms of complex biochemical network function is becoming an important methodological tool in the systems biology era. Number of different approaches to model these networks have been utilized-- they range from analysis of static connection graphs to dynamical models based on kinetic interaction data. Dynamical models have a distinct appeal in that they make it possible to observe these networks in action, but they also pose a distinct challenge in that they require detailed information describing how the individual components of these networks interact in living cells. Because this level of detail is generally not known, dynamic modeling requires simplifying assumptions in order to make it practical. In this review Boolean modeling will be discussed, a modeling method that depends on the simplifying assumption that all elements of a network exist only in one of two states

A Determination Of The Speed Of Light By The Phase-Shift Method

A low-frequency, phase-shift method for the measurement of the speed of light has been developed. This technique gives results commensurate with other advanced laboratory methods. One advantage of this technique is that the apparatus is of reasonable size and most of the circuitry involves widely known amateur radio techniques. Furthermore, our use of a low modulating frequency permits use of the solid-state, electro-optical light shutter. This eliminates the rather dangerous liquid Kerr cell, thus making our apparatus more acceptable to application in the undergraduate advanced laboratory. From a pedagogical point of view, the student is allowed to use and become acquainted with the lock-in amplifier which is so commonly found in the modern research laboratory. Two rather novel techniques were employed in this apparatus. These were phase multiplication and mixing of the rf signal in the last stages of the photomultiplier. Our value for the speed of light in air was [formula omitted]. The accepted value of the speed of light in air to the same number of significant figures is [formula omitted]. © 1969, American Association of Physics Teachers. All rights reserved