Occurrence of Salmonella spp. in flies and foodstuff from pork butcheries in Kampala, Uganda

Food-borne diseases such as salmonellosis are a major cause of human gastroenteritis worldwide, especially in the developing world due to poor sanitary conditions. Flies feed on food and breed in feces and other organic material. As such they are known vectors of Salmonella spp. Given that pork consumption in Uganda is rapidly increasing while good food safety practices remain absent, this study aims to assess the occurrence of Salmonella spp. in pork butcheries as a contribution to improve hygiene. Seventy-seven pork butcheries out of 179 mapped in a previous survey in Kampala were randomly selected. From June–October 2014, samples of house flies, foodstuff and equipment were collected from all butcheries. Cultural isolation of Salmonella spp. was performed according to ISO 6579:2002. Among 693 samples, 64 (9%) tested positive for Salmonella enteritidis. Among the positives, 32% were samples of raw pork (25), 25% flies’ midguts (19), less than 9% water (7), tomatoes (6), cabbage (4), onions (2) and one case on roasted pork1, respectively. Positive flies coincided with contaminated foodstuff in 29% of the butcheries. All 154 samples from either butchers’ hands or their equipment were negative for Salmonella spp. The prevalence of S. enteritidis, especially on raw pork and in flies, illustrates the need for improving food safety in pork butcheries. Further research is required clarifying the gaps; especially the role of flies as microbiological carriers. In this context investigations are ongoing to identify Salmonella serotypes and their antimicrobial drug-resistance situation. However, these findings merit increased attention and can be used to improve knowledge, attitudes and practices amongst butchers. The research was carried out with the financial support of the Federal Ministry for Economic Cooperation and Development (BMZ), Germany, and the CGIAR Research Program on Agriculture for Nutrition and Health, led by the International Food Policy Research Institute, through the Safe Food, Fair Food project at ILRI. Martin Heilmann got a scholarship from the German Academic Exchange Service (DAAD)

Present and future use of antimicrobials in pigs in developing countries and case studies from Uganda and Vietnam

Demand for pork is growing rapidly in developing countries, and will be mostly met by intensive production. Although this can produce large quantities of affordable meat, it can have environmental, social and human health externalities. We report on recent studies conducted by ILRI and partners on antimicrobial use in pork production in developing countries and antimicrobial resistance (AMR) in pork

Antimicrobial resistance of Salmonella enterica in pork and vegetable servings at pork joints in Kampala, Uganda

The aim of this study was to investigate the prevalence and antimicrobial resistance of Salmonella enterica subsp. enterica serovars including the presence of shared plasmids in pork and related fresh vegetables served in pork joints in Kampala, Uganda. Pork butcheries in three of the five administrative divisions of Kampala were included for the survey. Samples included raw pork, roasted pork, water, onions, tomatoes, cabbage, butcher’s hands (swabs), utensils (swabs) and fly midgut extracts. A total of 693 samples were collected from 77 pork butcheries from June- October 2014. Overall 53.2% pork joints had samples positive for Salmonella enterica subsp. enterica serovars. Isolation rates ranged from 31.2% (24/77) for raw pork, 1.3% (1/77) for roasted pork, 7.8% (6/77) for tomatoes, 2.6% (2/77) for onions, 5.2% (4/77) for cabbage, 9.1% (7/77) in water, and 22.1% (17/77) from fly midguts. Swab samples taken from utensil and butcher’s hands swabs were found negative (Heilmann et al., 2015). In the isolates obtained, resistance towards 22 antibiotics was tested. Resistances were found towards 11 out of the 22 antibiotics tested. High resistances were found to Cephazolin (97%), Cefotixime (93%), Gentamicin (88%), and Ceftazidime (86%). Intermediate resistance was found to Ciprofloxacin (59%) and Amoxicillin-Clavulanic acid (57.6%). Most isolates (85%) were susceptible to Levofloxacin, Ofloxacin, Sulfamethoxazole and Trimethoprim. Identification of plasmids by PCR-based replicon typing was performed recognizing FIA,FIB,FIC,HI1,HI2,I1- 1ᵞ,L/M,N,P,W,T,A/C,K,B/O,X,Y,F and FIIA. Six incompatibility groups were identified: FIA, W, FIC, FIB, P, Y with more than one incompatibility group existing among different isolates. A high resistance rate among Salmonella strains was found while the total number of incompatibility groups detected was with approximation 2.4. Thus, even though the total number of plasmids per strain is low, resistance rates detected remain high. The high resistance rates are probably resulting from intensified food animal production driving a greater use of antibiotics, which is a crucial aspect of public health concern

Eradication of human African trypanosomiasis? Don't forget the pigs!

Animal African Trypanosomosis (AAT) and Human African Trypanosomosis (HAT), caused by a protozoa of the genus Trypanosoma (section Salivaria), are both diseases of significant importance to sub-Sahara Africa. Several species of Trypanosoma are found in Africa but only two subspecies of T. brucei, are relevant to humans. They either cause the chronic (T. b. gambiense) or the acute (T. b. rhodesiense) form of human sleeping sickness, both with a fatal outcome if left untreated. While wildlife have long been known to be reservoirs for both HAT and AAT and show no clinical symptoms, infection in livestock, especially cattle, causes severe losses to local producers and are potential reservoirs for rhodesiense-HAT. Domestic pigs, too, are preferred hosts of Glossina spp. and have been reported to be potential reservoirs of both forms of HAT. Pigs are increasingly important as a source of income and food for smallholder livestock farmers in East Africa, especially in Uganda where both forms of HAT as well as AAT are endemic. Except for T. suis and T. brucei gambiense, all Trypanosoma species known to infect pigs have been reported from pigs in Uganda. The authors present findings from a review on the potential role of pigs as a livestock reservoir for HAT. We will discuss how and why infection with Trypanosoma spp. in pigs should be considered in differential diagnoses in clinically sick animals as well as in national HAT surveillance and eradication programs