Natural History Specimen Collections

The Fish Collection described in this document was transferred in 1992 to the Fish Collection of the University of Texas at Austin in Austin (at that time administratively in the Texas Memorial Museum, but at the time of publication of this digital version of the document it is one (https://biodiversity.utexas.edu/resources/collections/ichthyology) of the Biodiversity Collections in the University's Biodiversity Center (https://biodiversity.utexas.edu/). Thus, the data on the specimens in the Fish Collection described here are now included in the data published to GBIF (https://www.gbif.org/dataset/6080b6cc-1c24-41ff-ad7f-0ebe7b56f311) and other global biodiversity data aggregators by the UT Biodiversity Center's Fish Collection. The same data are also included in the Fishes of Texas Project (http://fishesoftexas.org - Hendrickson, Dean A., and Adam E. Cohen. 2015. “Fishes of Texas Project Database (Version 2.0)” doi:10.17603/C3WC70). Some of the UTMSI Fish Collection specimens remained at the UT Marine Science Insitute on long-term loan from 1992 until sometime in 2017 when the MSI disposed of all remaining specimens (including also Invertebrates). Some were disposed of by a professional HazMat company, but many were apparently taken to the Smithsonian Institution's (USNM) division of Invertebrate Biology. The fate of fish specimens, however, remains unresolved, but the Ichthyology Division at USNM had no knowledge of them at the time this report was archived here. Dean A. Hendrickson, Curator of Ichthyology, UT Austin, May 1, 2019The University of Texas Marine Science Institute, Port Aransas Marine Laboratory, Natural History Collection of Marine Organisms was initiated in the mid-1940's. Since that time specimens have been added from studies in the Gulf of Mexico, associated estuaries, and marine-influenced terrestrial habitats, with emphasis on the Texas and Mexico coasts. These studies were the baseline surveys for this area and have resulted in the collection of valuable marine organisms. The collection now holds approximately 5,000 catalogued specimens including all forms of biota, vertebrates, invertebrates, algae and flowering plants. The collection includes the marine fish and invertebrate specimens of the Texas Parks & Wildlife Department, incorporated into the UTMSI-PAML collection in 1976. This state collection, numbering 3,000, is composed primarily of Texas Gulf coast species of fish and invertebrates, representing baseline surveys conducted by the state fisheries biologists. With this collection are card catalogues by specimen number and phylogenetic order. Other collections incorporated are those from R/V Oregon cruises (U.S. Fish & Wildlife Service, Bureau of Commercial Fisheries, exploratory fishing vessel), H. H. Hildebrand's faunal surveys of the brown and pink shrimp grounds , Whitten et al.'s faunal survey of Texas coast jetties , J. W. Hedgepeth’s specimens collected during numerous faunal surveys, tide trap studies, and vegetation and algal surveys. Noteworthy studies from which specimens have been added to the collection are listed in Table 1. The museum collection is housed in an air-conditioned building in a room specifically designed for this collection. The present facility contains 924 square feet with 1089 square feet of shelf space, which can be trebled to accommodate BLM collections. There are at present 32 1' x 12' shelves, totaling 384 square feet; 194 18" x 12-1/2" shelves, totaling 303 square feet; and 120 11-1/2" x 42" shelves, totaling 402 5 square feet. The collection is a working museum open to the scientific community; specimens are available on loan to members of this community. Specimens may be used by visiting researchers, graduate-level students, professors, and classes. Attached is an invoice form, "Invoice of Specimens" - a standard form used for loan of collection items. The Port Aransas Marine Laboratory is committed to the continuing curation of specimens and will continue to provide the supplies necessary to maintain the natural history specimen collections. The University of Texas Marine Science Institute, Port Aransas Marine Laboratory is willing to incorporate the Bureau of Land Management collection into its collection. Funding is requested from BLM to obtain and incorporate all archived BLM specimens from the S.T.O.C.S. survey into the collection.Integrative Biolog

Peroxisomes in intestinal and gallbladder epithelial cells of the stickleback, Gasterosteus aculeatus L. (Teleostei)

The occurrence of microbodies in the epithelial cells of the intestine and gallbladder of the stickleback, Gasterosteus aculeatus L., is described. In the intestine the organelles are predominantly located in the apical and perinuclear zone of the cells and may contain small crystalline cores. In gallbladder epithelial cells the microbodies are distributed randomly. The latter organdies are characterized by the presence of large crystalloids. Cytochemical and biochemical experiments show that catalase and D-amino acid oxidase are main matrix components of the microbodies in both the intestinal and gallbladder epithelia. These organelles therefore are considered peroxisomes. In addition, in intestinal mucosa but not in gallbladder epithelium a low activity of palmitoyl CoA oxidase was detected biochemically. Urate oxidase and L-α hydroxy acid oxidase activities could not be demonstrated.

Measurement Properties of the Full and Brief Version of the Work Rehabilitation Questionnaire in Persons with Physical Disabilities

Purpose The Work Rehabilitation Questionnaire (WORQ) is a self-report vocational rehabilitation assessment. A comprehensive (WORQ-FULL) and a brief version (WORQ-BRIEF) are available. The purpose of this study was to investigate measurement properties of both versions in persons with physical disabilities. Methods Cross sectional and test-retest design. Adults with physical disabilities in vocational rehabilitation were included. Internal consistency (Cronbach's alpha), test-retest reliability (intra-class correlation; ICC), agreement between sessions (Bland-Altman Plots), criterion validity (ICC and agreement with Bland-Altman Plots between WORQ-FULL and WORQ-BRIEF) and convergent validity with the Work Ability Index -Single item (WAS) and the EuroQOL 5D-5L were analyzed. Results Out of the 91 individuals who agreed to participate, 74 (81%) returned questionnaire T1 and 49 (54%) participants returned questionnaire T2 within the maximum time interval (= 27 days). At T2, 28 (57%) participants reported no medical changes compared to T1. Median age was 49 (IQR 40-60), 57% were male, 47% had experienced a stroke and 27% a spinal cord injury (n = 49). Internal consistency was good: 0.95/0.95/0.94 for the WORQ-FULL and 0.88/0.89/0.85 for the WORQ-BRIEF (n = 74/n = 48/n = 28, respectively). Test-retest reliabilitywas good: ICC = 0.86/0.85 for the WORQ-FULL and ICC = 0.87/0.86 for the WORQ-BRIEF (n = 49/ n = 28). Bland Altman plots suggested a higher score at T1. As for criterion validity of the WORQ-FULL versus the WORQ-BRIEF, ICC was good (ICC = 0.84; n = 74), however Bland Altman plots indicated potential bias. Correlations with the WAS/EuroQOL 5D-5L were variable: r = -0.24/r = -0.57 (WORQ-FULL) and r = -0.28/-0.65 (WORQ-BRIEF). Conclusions The WORQ showed good internal consistency and test-retest reliability. Agreement demonstrated large score differences are needed to indicate change beyond random chance at individual level, whereas small changes are sufficient at group level. Criterion validity of the WORQ-FULL versus the WORQ-BRIEF was supported, however, agreement demonstrated moderate to large score differences are needed to indicate change beyond random chance at individual level, whereas small changes are sufficient at group level. This indicates the WORQ-FULL and WORQ-BRIEF are better not used interchangeably. Correlation analyses provided better insight in the validity of the WORQ. Convergent validity was supported for the WORQ-BRIEF with the EuroQoL 5D-5L (r = -0.65)

Challenge pools of hepatitis C virus genotypes 1-6 prototype strains: replication fitness and pathogenicity in chimpanzees and human liver-chimeric mouse models

Chimpanzees represent the only animal model for studies of the natural history of hepatitis C virus (HCV). To generate virus stocks of important HCV variants, we infected chimpanzees with HCV strains of genotypes 1-6 and determined the infectivity titer of acute-phase plasma pools in additional animals. The courses of first- and second-passage infections were similar, with early appearance of viremia, HCV RNA titers of >10(4.7) IU/mL, and development of acute hepatitis; the chronicity rate was 56%. The challenge pools had titers of 10(3)-10(5) chimpanzee infectious doses/mL. Human liver-chimeric mice developed high-titer infections after inoculation with the challenge viruses of genotypes 1-6. Inoculation studies with different doses of the genotype 1b pool suggested that a relatively high virus dose is required to consistently infect chimeric mice. The challenge pools represent a unique resource for studies of HCV molecular virology and for studies of pathogenesis, protective immunity, and vaccine efficacy in vivo

Rare presentation of a squamous cell carcinoma originating from gastric compartment 1 in an alpaca = Bijzondere presentatie van een squameus celcarcinoom afkomstig vancompartiment 1 van de maag bij een alpaca

A twenty-year-old, male, intact alpaca with a history of anorexia, progressive weakness and recumbency was euthanized because of a poor prognosis and clinical deterioration. The animal was submitted for necropsy at the pathology department of Dierengezondheidszorg Vlaanderen (DGZ) diagnostic lab. A full necropsy was performed and showed a large firm white mass measuring 18x8x10 cm attached to the dorsal wall of gastric compartment 1 (C1) and expanding to the abdominal aorta. Miliary small white metastatic nodules were present on the pleura and peritoneum (carcinomatosis). Multiple small white metastatic nodules were also present in the parenchyma of the liver. On histological investigation, this neoplasia was characterized as squamous cell carcinoma (SCC)

Content validity of the Work Rehabilitation Questionnaire (WORQ) for persons with spinal cord injury:A mixed methods study

STUDY DESIGN: Mixed methods. OBJECTIVES: The aim of our study was to investigate the content validity of the Work Rehabilitation Questionnaire (WORQ) for use in persons with post-acute and chronic spinal cord injury (SCI). SETTING: A university-based Rehabilitation Center in The Netherlands. METHODS: Contents of the WORQ, brief ICF core sets for SCI for post-acute care and for chronic situation were compared with semi-guided interviews with persons with SCI and controlled for relevance by SCI rehabilitation professionals in two group meetings. RESULTS: Fourteen interviews with persons with SCI were performed. Two group meetings with 8 and 9 SCI rehabilitation professionals were held. Thirty seven of the 46 ICF categories (80%) of the WORQ were confirmed by both sources: mentioned in interviews with persons with SCI and considered important by the SCI professionals. The remaining 9 categories (20%) were confirmed by either the persons with SCI or the SCI professionals. Fourteen ICF categories that are part of the brief ICF core set for SCI for acute care and/or chronic situation, however are not part of the WORQ, have revealed importance by persons with SCI and SCI professionals. CONCLUSION: Our study confirms that most categories of the WORQ are important to consider for VR in persons with SCI, however, there are ICF categories that are absent in the WORQ and deemed relevant for use in VR in persons with SCI. Consequently, the content validity of the WORQ without additional items is insufficient for persons with SCI