Larvi '95 : fish & shellfish larviculture symposium

This 521 pages Special Publication No 24 of the European Aquaculture Society (EAS) contains the abstracts of the oral papers and the short communications (2 to 4 pages per article) of the poster contributions

Total replacement of fish oil with vegetable oils in the diet of juvenile jade perch scortum barcoo reared in recirculating aquaculture systems

status: publishe

Apomorphine-induced growth hormone release in African catfish (Clarias gariepinus) is related to the animal's nutritional status

In African catfish the dopamine agonist apomorphine (APO) stimulates in vivo growth hormone (GH) release. The present study demonstrates that the potency of APO to stimulate plasma GH levels is affected by the nutritional status of the fish. The effect of starvation on APO induced GH release was investigated in sexually mature and immature catfish. Administration of APO clearly stimulated plasma GH levels in mature catfish that had been starved for 3 and 5 weeks, while no effect could be observed prior to starvation. This increased responsiveness to the GH stimulating action of APO was also demonstrated in fasted immature fish, though it was only evident in fish starved for a prolonged period (19 days or more). The importance of the duration of the starvation period suggests that the enhanced responsiveness is the result of physiological adaptations to starvation rather than an acute effect of food deprivation.status: publishe

Toxicology studies with recombinant staphylokinase and with SY 161-P5, a polyethylene glycol-derivatized cysteine-substitution mutant

SY 161-P5, a polyethylene glycol derivatized (PEGylated) mutant of the recombinant Staphylokinase (rSak) variant SakSTAR, exhibiting reduced antigenicity is in clinical development for treatment of acute myocardial infarction as a single bolus injection. A series of safety studies were performed in vivo as a routine toxicology program with SY 161-P5 (PEG-rSakSTAR) and with the recombinant Staphylokinase variant Sak42D (rSak42D). For both compounds, intravenous single bolus injections of up to 100-fold therapeutic equivalent, as well as repeated injections during 7 to 28 days revealed no significant pathological findings in mice, rats or hamsters. However, New Zealand white rabbits developed clinically silent, multifocal myocarditis following single or repeat doses of SY 161-P5 or of Sak42D. These findings were dose-independent and reversible. A similar species-specific cardiotoxic effect has previously been described for other proteolytic proteins, including the approved drugs Streptokinase and Acetylated Plasminogen Streptokinase Complex (APSAC). The large experience with these drugs, as well as the clinical data accumulated both with PEGylated and non-PEGylated rSak variants to date, do not indicate cardiotoxic hazards associated with the use of these drugs in humans.status: publishe

Schizochytrium as a replacement for fish oil in a fishmeal free diet for jade perch, Scortum barcoo (McCulloch & Waite)

© 2016 John Wiley & Sons Ltd. A 10-week trial was conducted to determine the response of juvenile jade perch Scortum barcoo on the replacement of dietary fish oil (FO) in a fishmeal free diet. Three iso-nitrogenous, isocaloric and isolipidic diets were formulated, each containing a different primary fat source: FO, linseed oil (LO), and a mixture of Schizochytrium and LO. The substitution of FO with the mixture of Schizochytrium and LO did not cause a difference in growth. However, there was an 8% reduction in weight gain in fish fed dietary LO, indicating that juvenile jade perch do require a minimal concentration of dietary n-3 highly unsaturated fatty acids (HUFA). Fish fed the Schizochytrium diet stored more efficient n-3 HUFA and in particular DHA in their flesh, and retained a higher fillet recovery compared to fish fed FO. In addition, we demonstrated that jade perch are able to produce both n-3 HUFA and n-6 HUFA when dietary PUFA are present. Fish fed the LO diet for 10 weeks contained the lowest amount of n-3 HUFA in fillets among dietary treatment groups. However, feeding these fish the Schizochytrium diet for an additional 4 weeks increased the n-3 HUFA content towards the same concentration of n-3 HUFA found in the flesh of fish fed FO, without affecting the sensory properties of the fillets. In contrary, feeding the Schizochytrium diet for a continuous period of 14 weeks lowered overall sensory property scores.status: publishe