Microvesicles produced by natural killer cells of the NK-92 cell line affect the phenotype and functions of endothelial cells of the EA.Hy926 cell line

Microvesicles (MVs) are small (100-1000 nm) subcellular structures produced by both motionless and activated cells that can transfer molecules to target cells, and regulate physiological and pathological processes. MVs of leukocyte origin, in particular those produced by natural killer cells (NK cells), remain the least studied population of MVs. NK cells can change the functional activity of endothelial cells (ECs) and are involved in regulating angiogenesis. The ability of NK cell-derived MVs to influence the functionality of ECs is understudied currently. We aimed to study the effect of MVs produced by NK cells of the NK-92 cell line on the phenotype, caspase activity, proliferation and migration of ECs of the EA.Hy926 cell line. We cultured ECs in the presence of MVs derived from the NK-92 cell line, and then used flow cytometry to assess changes in EC phenotype, intracellular protein transfer from MVs to ECs, and the relative death of ECs. We used western blot analysis to evaluate the expression of granzyme B in NK cells and in the MVs that they produced, as well as the expression of granzyme B, caspases, extracellular-regulated kinase (ERK) and protein kinase B (AKT) in ECs. We also assessed the proliferation and migration of ECs in the presence of MVs derived from cells of the NK-92 cell line. The results revealed significant differences in the proteomic profiles of cells of the NK-92 cell line and their MV product. Contact between ECs and MVs derived from cells of the NK-92 cell line is accompanied by the following events: a) expression of granzyme B in ECs, b) activation of caspase-9 and caspase-3, with partial EC death, c) appearance of the panleukocyte marker CD45 on ECs, d) decrease in CD105 expression, and increase in CD34 and CD54 expression, and e) inhibition of EC migration. Transfer of ERK (but not AKT) from MVs derived from cells of the NK-92 cell line to ECs, at a concentration 10 times lower than that which causes EC death, leads to an increase in EC proliferation

Splitting of antiferromagnetic resonance modes in the quasi-two-dimensional collinear antiferromagnet Cu(en)(H2O)(2) SO4

A low-temperature magnetic resonance study of the quasi-two-dimensional antiferromagnet Cu(en)(H2O)(2)SO4 (en = C2H8N2) was performed down to 0.45 K. This compound orders antiferromagnetically at 0.9 K. The analysis of the resonance data within the hydrodynamic approach allowed us to identify anisotropy axes and to estimate the anisotropy parameters for the antiferromagnetic phase. Dipolar spin-spin coupling turns out to be the main contribution to the anisotropy of the antiferromagnetic phase. The splitting of the resonance modes and its nonmonotonous dependence on the applied frequency were observed below 0.6 K in all three field orientations. Several models are discussed to explain the origin of the nontrivial splitting, and the existence of inequivalent magnetic subsystems in Cu(en)(H2O)(2)SO4 is chosen as the most probable source.Web of Science1011art. no. 01441

Main elements of taxation in the conditions of the development of digital economy

The purpose of this article is to consider the main elements of taxation in a digital economy. On the base of the analysis, it was concluded that in conditions of digital economy, the development of information resources would provide a concretization of the provisions of the main elements of taxation and optimize the emergence and pursuance of the constitutional duties to pay taxes, as well as the calculation of tax amounts. The main provisions and conclusions can be used in scientific and practical activities when considering the issues of the legal construction of taxes.El propósito de este artículo es la consideración de los principales elementos de los impuestos en una economía digital. Sobre la base del análisis del estudio, se concluyó que, en condiciones de economía digital, el desarrollo de los recursos de información proporciona una concreción de las disposiciones de los principales elementos de la tributación y optimiza la aparición y el cumplimiento de los deberes constitucionales de pagar impuestos., así como el cálculo de los importes de impuestos. Las principales disposiciones y conclusiones pueden utilizarse en actividades científicas y prácticas al considerar los problemas de la construcción legal de impuestos

Potentiation effect of the AMPK activator A-769662 on cardiac myocytes metabolism and survival

Abstract 286 van Poster session 2 Frontiers in CardioVascular Biology, London 30th March – 1st April 2012 Second Congress of the ESC Council on Basic Cardiovascular Science