Effects of elevated CO2 concentrations and fly ash amended soils on trace element accumulation and translocation among roots, stems and seeds of Glycine max (L.) Merr.

The carbon dioxide (CO2) levels of the global atmosphere and the emissions of heavy metals have risen in recent decades, and these increases are expected to produce an impact on crops and thereby affect yield and food safety. In this study, the effects of elevated CO2 and fly ash amended soils on trace element accumulation and translocation in the root, stem and seed compartments in soybean [Glycine max (L.) Merr.] were evaluated. Soybean plants grown in fly ash (FA) amended soil (0, 1, 10, 15, and 25% FA) at two CO2 regimes (400 and 600 ppm) in controlled environmental chambers were analyzed at the maturity stage for their trace element contents. The concentrations of Br, Co, Cu, Fe, Mn, Ni, Pb and Zn in roots, stems and seeds in soybeans were investigated and their potential risk to the health of consumers was estimated. The results showed that high levels of CO2 and lower concentrations of FA in soils were associated with an increase in biomass. For all the elements analyzed except Pb, their accumulation in soybean plants was higher at elevated CO2 than at ambient concentrations. In most treatments, the highest concentrations of Br, Co, Cu, Fe, Mn, and Pb were found in the roots, with a strong combined effect of elevated CO2 and 1% of FA amended soils on Pb accumulation (above maximum permitted levels) and translocation to seeds being observed. In relation to non-carcinogenic risks, target hazard quotients (TQHs) were significant in a Chinese individual for Mn, Fe and Pb. Also, the increased health risk due to the added effects of the trace elements studied was significant for Chinese consumers. According to these results, soybean plants grown for human consumption under future conditions of elevated CO2 and FA amended soils may represent a toxicological hazard. Therefore, more research should be carried out with respect to food consumption (plants and animals) under these conditions and their consequences for human health.Fil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Klumpp, A.. Universität Hohenheim. Institute of Landscape and Plant Ecology; AlemaniaFil: Fangmeier, A. Universität Hohenheim. Institute of Landscape and Plant Ecology; AlemaniaFil: Pignata, Maria Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Biomonitoring of atmospheric trace elements in agricultural areas and a former uranium mine

Most biomonitoring studies worldwide have evaluated the air quality in industrial and urban areas, and even in mining areas to a lesser extent. However, air quality investigations in agricultural areas are scarce. In the present study, the trace metal accumulation and physiological response of the biomonitor Tillandsia capillaris were assessed. Plant samples were transplanted to a reference site, a former open-cast uranium mine, and agricultural sites with varying pollution levels (from normal agricultural practices and near an open rubbish dump) in the province of Córdoba, Argentina. Biomonitors were exposed to ambient air for different exposure periods for physiological or trace element determination. The bioindicators revealed that the highest physiological damage occurred at the sites close to the open dump and the former uranium mine, while a comparison among exposure periods indicating the winter season produced the highest physiological damage in the biomonitor due to the adverse climatic conditions and air pollution. As the trace metal accumulation in the biomonitor was mainly associated with the open dump and uranium mine sites, monitoring and remediation programs should now be applied to these sites in order to alleviate the negative effects of pollution on the environment and the population.Fil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Wannaz, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; ArgentinaFil: Weller, Sebastian Benjamin. Karlsruher Institut Fur Technologie; AlemaniaFil: Pignata, María Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Argentin

Biomonitoring of airborne fluoride and polycyclic aromatic hydrocarbons in industrial areas of Cordoba, Argentina, using standardized grass cultures of Lolium multiflorum

A biomonitoring study was performed employing standardized grass cultures. Plants of Lolium multiflorum were exposed at 4 industrial sites over three?month periods in two seasons (dry and rainy) and the biomass produced was used for subsequent measurements of fluoride, polycyclic aromatic hydrocarbons (phenanthrene, anthracene, pyrene, benzo[a]anthracene, chrysene, benzo[b]fluoranthene, benzo[k]fluoranthene, benzo[a]pyrene, dibenzo[a,h]‐ anthracene and benzo[g,h,i]perylene), total chlorophyll, malondialdehyde, water, and sulfur content. The total content of polycyclic aromatic hydrocarbons (PAHs) revealed seasonal variations, with the highest values corresponding to the dry season, although this species showed a high retention capacity of PAHs during rainy season. In addition, sampling sites with high vehicular traffic and metal?mechanical industries were associated with the highest content of PAHs. Furthermore, physiological degradation associated with anthropogenic activities in the sampling sites was observed. Fluoride content in the biomonitor was associated with the production and use ofcement, which was higher in the dry season.Fil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Wannaz, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Franzaring, Jurgen. Universidad de Hohenheim. Instituto de Paisaje y Ecología Vegetal. Especialidad En Ecología Vegetal y Ecotoxicologia; AlemaniaFil: Klumpp, Andreas. Universidad de Hohenheim. Instituto de Paisaje y Ecología Vegetal. Especialidad En Ecología Vegetal y Ecotoxicologia; AlemaniaFil: Fangmeier, Andreas. Universidad de Hohenheim. Instituto de Paisaje y Ecología Vegetal. Especialidad En Ecología Vegetal y Ecotoxicologia; AlemaniaFil: Pignata, Maria Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Multidisciplinary study of chemical and biological factors related to lead accumulation in sorghum crops grown in contaminated soils and their toxicological implications

In this study, the content of Pb, the physico-chemical and biological parameters in soils, and the metal transfer to vegetative and reproductive Sorghum bicolor plants were evaluated along with their relationship with the toxicological risk of crop consumption. To carry this out, soil and sorghum samples at different growth stages were collected near to a former battery recycling plant. The results showed that the concentrations of Pb in soils at several sites were above the maximum permissible levels. Metal bioavailability was not directly related to the pH, OM% or EC, while no association between metals and the different genera of fungi was observed. Sorghum crops accumulated Pb mainly in the roots in all of the growth stages, and therefore presented low levels of Pb in aerial parts without toxicological risk due to direct consumption. Taken together, our results revealed that sorghum could be employed as a potential phytostabilizator of lead in soils associated with crop production. However, further studies are necessary to extend these findings.Fil: Blanco, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Salazar, María Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Vergara Cid, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pereyra, Carina Maricel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Cavaglieri, Lilia Reneé. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina. Universidad Nacional de Río Cuarto; ArgentinaFil: Becerra, Alejandra Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pignata, Maria Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Aplicación de enmienda de compost de biosólidos como método de mitigación para reducir estrés y acumulación de plomo en plantas de soja

La incorporación de metales pesados en suelos agrícolas tiene efectos adversos sobre la producción, calidad de los cultivos y la salud de los consumidores. El estrés ocasionado por exposición a metales pesados eleva la producción de especies reactivas del oxígeno (EROs) y peroxidación lipídica. Para combatir estos efectos, las plantas poseen mecanismos de defensas enzimáticos y no enzimáticos. El objetivo de este trabajo fue evaluar la eficiencia de una enmienda orgánica para reducir la acumulación de plomo (Pb) y el estrés en plantas de soja. Para ello se utilizó un diseño experimental en invernadero utilizando 3 niveles de enmienda a partir de compost de biosólidos: 0%; 5%; 10% en suelos contaminados con 650 mg/Kg de Pb, y en un suelo control (Pb 20 mg/Kg). La soja creció en macetas y completó su ciclo de vida hasta la producción de frutos. Se evaluó la acumulación de Pb en los distintos órganos y la respuesta al estrés por métodos enzimático: Glutation S-transfersa (GST) y no enzimáticos: MDA, clorofilas, carotenos, azúcares, proteínas y capacidad antioxidante total (FRAP). La aplicación de la enmienda al 10% en suelos con Pb resultó efectiva ya que en soja mejoró la producción de granos en calidad y biomasa, al mismo tiempo que disminuyó significativamente la acumulación de Pb en raíces, tallos y hojas. En nódulos y granos se observaron menores concentraciones pero no fueron significativas. En los suelos control la aplicación de enmienda mejoró la biomasa desarrollada e incrementó notablemente los indicadores relacionados a la productividad como lo son clorofilas, proteínas y azúcares. En los suelos con Pb, el agregado de enmienda al 10% redujo el estrés en las plantas obteniéndose una disminución en la respuesta antioxidante FRAP y actividad GST, ya que al estar menos disponible el Pb en suelos y reducirse la acumulación en plantas se requirió menor respuesta antioxidante. Si bien no hubo cambios en la concentración de azúcares y proteínas, mejoró la concentración de clorofilas y la eficiencia del fotosistema II, indicando mejoras en la productividad y menor daño en el sistema fotosintético que es descripto como sensible al estrés ocasionado por metales. Se concluye que la aplicación de enmienda orgánica al 10% de compost a partir de biosólidos resultó efectiva en la reducción de Pb y del estrés en soja, pudiendo ser un potencial método de mitigación de la contaminación de suelos con plomo.Fil: Vergara Cid, Carolina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Ferreyroa, Gisele Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaVII Congreso Argentino de la Sociedad de Toxicología y Química Ambiental de ArgentinaSan LuisArgentinaSociedad de Toxicología y Química Ambienta

Effect of Pb-Polluted soil on soybean growth and associated toxicological risk

Glycine max (L.) Merr. (soybean) has been mentioned as a potential accumulator of hazardous metals, such as Pb. The main route of human exposure to heavy metals is consumption. This study evaluates Pb accumulation in soybean at different growth stages. The aim was to determine the period of the crop development when absorption and distribution mostly occur. Soybean plants were grown in control and Pb-polluted soils in a greenhouse experiment. Morpho-physiological parameters and Pb content in organs were analyzed. Results showed that Pb affected the biomass of roots and plant height, with the highest Pb accumulation occurring in the roots and with low translocation to aerial organs. Moreover, Pb accumulation and distribution occurred before grain filling, the crop critical period. Soybean seeds accumulated Pb above permissible values, but with no associated toxicological risk. Furthermore, pods showed higher Pb values ​​than seeds, suggesting a protective effect.Fil: Blanco, Andrés. Universidad Nacional de Córdoba; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pignata, Maria Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba; ArgentinaFil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Córdoba; Argentin

Comparison of the air pollution biomonitoring ability of three Tillandsia species and the lichen Ramalina celastri in Argentina

Bioaccumulation ability and response to air pollution sources were evaluated for Tillandsia capillaris Ruíz and Pav. f. capillaris, T. recurvata L., T. tricholepis Baker and the lichen Ramalina celastri (Spreng.)Krog. and Swinsc. Epiphyte samples collected from a non contaminated area in the province of Córdoba were transplanted to a control site and three areas categorised according to agricultural, urban and industrial (metallurgical and metal-mechanical) emission sources. Bioindicators were exposed for 3-, 6- and 9-month periods. A foliar damage index was established for Tillandsia and a pollution index for the lichen, and S, Fe, Mn and Zn concentrations were determined. An order of efficiency for the species and conditions studied is proposed taking into account heavy metal accumulation: T. recurvata > T. tricholepis >R. celastri >T. capillaris. All species studied showed Mn to be related to agricultural activity and Fe to industries and soil particles, and Zn was related to urban and industrial sources. As far as physiological response is concerned, T. tricholepis and T. capillaris were more sensitive to agricultural activities, whereas T. recurvata was sensitive to urban and industrial sources, and only partially to agricultural sources. No relationship was found for R. celastri.Fil: Bermudez, Gonzalo Miguel Angel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pignata, Maria Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Accumulation of polycyclic aromatic hydrocarbons and trace elements in the bioindicator plants Tillandsia capillaris and Lolium multiflorum exposed at PM10 monitoring stations in Stuttgart (Germany)

The accumulation of polycyclic aromatic hydrocarbons (PAHs) in Tillandsia capillaris Ruiz and Pav. form capillaris and trace elements in T. capillaris and Lolium multiflorum (LAM) cv. Lema was assessed and evaluated in the city of Stuttgart, Germany. Several sites (urban, suburban and rural) categorized according to type and intensity of vehicular traffic were investigated. At these sites, plants of T. capillaris and standardized cultures of L. multiflorum were exposed to ambient air. Foliar concentrations of PAHs (16 priority pollutants according to US-EPA) and of the trace elements Br, Co, Cu, Fe, Mn, Ni, Pb and Zn were determined. A high level of vehicular traffic was associated with the largest concentrations of PM10 in ambient air and with the highest contents of PAHs and heavy metals in the bioindicator plants. The results showed a similar pattern between T. capillaris and the standardized biomonitor L. multiflorum. Therefore, these results allow us to propose T. capillaris as a suitable bioindicator to assess the distribution of pollution impacts caused by PAHs and trace elements in different subtropical and tropical regions.Fil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pignata, María Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Fangmeier, A.. Universidad de Hohenheim; AlemaniaFil: Klumpp, A.. Universidad de Hohenheim; Alemani

Assessment of lead tolerance on Glycine max (L.) Merr. at early growth stages

Lead (Pb) contamination of agricultural soils, and subsequently of crops, has been widely reported. Soybean (Glycine max (L.) Merr.) has been indicated as a plant that accumulates Pb, even in soils that do not exceed the maximum permissible levels. Considering the toxicity of this heavy metal, the aim of the present study was to assess different concentrations of Pb, from low to extremely high (0.25 mM, 1 mM, and 2.5 mM), in soybean seedlings and their tolerance by analyzing morpho-physiological parameters in hydroponic experiments. Soybean seedlings were exposed to control and Pb treatments during 8 days, coinciding with the early growth stages, and the following variables were analyzed: biomass, Pb content in roots, stems and leaves, photosynthetic efficiency, leaf area, biochemical response (antioxidant power, chlorophylls, malondialdehyde), and relative water content of leaves. Results showed that roots accumulated much more Pb than the other organs, with Pb accumulation in roots being saturated even at the lowest Pb concentration, which was reflected in root biomass. Moreover, absorption of culture solutions was lower in Pb treatments, which was also reflected in the lower leaf relative water content. Lead toxicity symptoms in leaves (chlorosis and dark spots, and a decrease of biomass and leaf area, chlorophyll content, and photosynthetic efficiency), and an increase of the oxidative defense system were associated only with the highest Pb concentration (2.5 mM). Our findings support the evidence of soybean as a species tolerant to Pb, showing the effects of toxicity at very high concentrations.Fil: Blanco, Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Pignata, Maria Luisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; ArgentinaFil: Lascano, Hernán R.. Instituto Nacional de Tecnologia Agropecuaria. Centro de Investigaciones Agropecuarias. Unidad de Estudios Agropecuarios. - Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Cordoba. Unidad de Estudios Agropecuarios.; ArgentinaFil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentin

Assessment of polycyclic aromatic hydrocarbons in industrial and urban areas using passive air samplers and leaves of Tillandsia capillaris.

This study extends the current knowledge regarding the use of passive air samplers(PAS)and biomonitoring with plants for the measurement of polycyclic aromatic hydrocarbons(PAHs), with the aim of evaluating the association between the accumulation of PAHs in leaves of Tillandsia capillaris and their concentrations in air measured by PAS. In addition, in order to study the seasonal variations of PAHs emitted by industries and vehicular traffic, a monitoring program was performed employing leaves of T. capillaris and polyurethane foam(PUF)disk passive air samplers. Results showed that both passive samplers and biomonitors presented the highest concentration values of PAHs in the dry season at sites with anthropic influence. Most of the PAHs were associated with urban and industrial sources present in the city of Córdoba. The concentration of ANT was associated with the presence of domestic sources; the use of natural gas as fuel for the cement plant was considered to be an important source of BaA and DBA enrichment was associated with vehicle emissions in this area. T. capillaris demonstrated to be a good accumulator of PAHs, considering that it reflected seasonal differences between different sites and that these were similar to those found in the passive samplers for the dry season. Therefore, these results contribute to the development of protocols for monitoring programs of PAHs that can be applied over large areas.Fil: Wannaz, Eduardo Daniel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Abril, Gabriela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Rodriguez, Judith Hebelen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Pignata, M. l.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentin