43,262 research outputs found
Recommended from our members
Vectorial targeting of an endogenous apical membrane sialoglycoprotein and uvomorulin in MDCK cells.
We studied the cell-surface delivery pathways of newly synthesized membrane glycoproteins in MDCK cells and for this purpose we characterized an endogenous apical integral membrane glycoprotein. By combining a pulse-chase protocol with domain-selective cell-surface biotinylation, immune precipitation, and streptavidin-agarose precipitation (Le Bivic et al. 1989. Proc. Natl. Acad. Sci USA. 86:9313-9317), we followed the appearance at the cell surface of a major apical sialoglycoprotein, gp114, a basolateral protein, uvomorulin, and a transcytosing protein, the polyimmunoglobulin receptor (pIg-R). We determined that both gp114 and uvomorulin appeared to be delivered directly to their respective surface, with mistargeting levels of 8 and 2%, respectively. Using the same technique, the pIg-R was first detected on the basolateral domain and then on the apical domain, to be finally released into the apical medium, as described (Mostov, K. E., and D. L. Deitcher. 1986. Cell. 46:613-621). To directly determine whether the gp114 pool present on the basolateral surface was a precursor of the apical gp114, we compared it with the equivalent pIg-R pool, by labeling with sulfo-NHS-SS-biotin, a cleavable, tight junction-impermeable probe, and by following the fraction of this probe that became resistant to basal glutathione and accessible to apical glutathione during incubation at 37 degrees C. We found that, contrary to pIg-R, basolateral gp114 was poorly endocytosed and was not transcytosed to the apical side. These results demonstrate that an endogenous apical integral membrane glycoprotein of Madin-Darby canine kidney cells is sorted intracellularly and is vectorially targeted to the apical surface
Signatures of macroscopic quantum coherence in ultracold dilute Fermi gases
We propose a double-well configuration for optical trapping of ultracold
two-species Fermi-Bose atomic mixtures. Two signatures of macroscopic quantum
coherence attributable to a superfluid phase transition for the Fermi gas are
analyzed. The first signature is based upon tunneling of Fermi pairs when the
power of the deconfining laser beam is significantly reduced. The second relies
on the observation of interference fringes in a regime where the fermions are
trapped in two sharply separated minima of the potential. Both signatures rely
on small decoherence times for the Fermi samples, which should be possible by
reaching low temperatures using a Bose gas as a refrigerator, and a bichromatic
optical dipole trap for confinement, with optimal heat-capacity matching
between the two species
Virtual Data in CMS Analysis
The use of virtual data for enhancing the collaboration between large groups
of scientists is explored in several ways:
- by defining ``virtual'' parameter spaces which can be searched and shared
in an organized way by a collaboration of scientists in the course of their
analysis;
- by providing a mechanism to log the provenance of results and the ability
to trace them back to the various stages in the analysis of real or simulated
data;
- by creating ``check points'' in the course of an analysis to permit
collaborators to explore their own analysis branches by refining selections,
improving the signal to background ratio, varying the estimation of parameters,
etc.;
- by facilitating the audit of an analysis and the reproduction of its
results by a different group, or in a peer review context.
We describe a prototype for the analysis of data from the CMS experiment
based on the virtual data system Chimera and the object-oriented data analysis
framework ROOT. The Chimera system is used to chain together several steps in
the analysis process including the Monte Carlo generation of data, the
simulation of detector response, the reconstruction of physics objects and
their subsequent analysis, histogramming and visualization using the ROOT
framework.Comment: Talk from the 2003 Computing in High Energy and Nuclear Physics
(CHEP03), La Jolla, Ca, USA, March 2003, 9 pages, LaTeX, 7 eps figures. PSN
TUAT010. V2 - references adde
Probing the role of single defects on the thermodynamics of electric-field induced phase transitions
The kinetics and thermodynamics of first order transitions is universally
controlled by defects that act as nucleation sites and pinning centers. Here we
demonstrate that defect-domain interactions during polarization reversal
processes in ferroelectric materials result in a pronounced fine structure in
electromechanical hysteresis loops. Spatially-resolved imaging of a single
defect center in multiferroic BiFeO3 thin film is achieved, and the defect size
and built-in field are determined self-consistently from the single-point
spectroscopic measurements and spatially-resolved images. This methodology is
universal and can be applied to other reversible bias-induced transitions
including electrochemical reactions.Comment: 34 pages,4 figures, high quality figures are available upon request,
submitted to Phys. Rev. Let
An Extended Radio Counterpart of TeV J2032+4130?
We carried out a 5-pointing mosaic observation of TeV J2032+4130 at 1.4 and
4.8 GHz with the VLA in April of 2003. The analysis of the 4.8GHz data indicate
weak wispy shell-like radio structure(s) which are at least partially
non-thermal. The radio data is compatible with one or more young supernova
remnants or perhaps the signature of large scale cluster shocks in this region
induced by the violent action of the many massive stars in Cyg OB2.Comment: Proc. 1st GLAST Symp. Feb 5-8, 2007, Stanford C
- …