Development And Characterization Of Microsatellite Markers For Hebanthe Eriantha (amaranthaceae)

Premise of the study: Hebanthe eriantha (Amaranthaceae) is extensively collected and used in folk medicine. Microsatellite markers were developed and characterized to investigate the genetic structure and diversity of germplasm collections of this species. Methods and Results: Eleven highly polymorphic microsatellite markers were developed. The number of alleles observed for each locus ranged from two to eight. The observed and expected heterozygosities ranged from 0.000 to 0.808 and 0.455 to 0.851, respectively. Conclusions: These results show the utility of microsatellite loci for studies of population genetics in H. eriantha, which are important for the future conservation and cultivation of this medicinal species. © 2011 Botanical Society of America.9810e282e283Billotte, N., Lagoda, P.J.L., Risterucci, A.-M., Baurens, F.-C., Microsatellite enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Creste, S., Tulmann Neto, A., Figueira, A., Detection of single sequence repeat polymorphisms in denaturing polyacrylamide sequencing gels by silver staining (2001) Plant Molecular Biology Reporter, 19, pp. 299-306Doyle, J.J., Doyle, J.L., Isolation of plant DNA from fresh tissue (1990) Focus (San Francisco, Calif.), 12, pp. 13-15Goudet, J., FSTAT (version 1.2): A computer program to calculate F-statistics (1995) Journal of Heredity, 86, pp. 485-486Lewis, P.O., Zaykin, D., (2000) Genetic data analysis: Computer program for the analysis of allelic data, , http://hydrodictyon.eeb.uconn.edu/people/plewis/software, Version 1.0 (d15). Free program distributed by the authors over the internet from the GDA. Website, [accessed March 2011]Marchioretto, M.S., Miotto, S.T.S., Siqueira, J.C., Padrões de distribui ção geográfica dos táxons brasileiros de Hebanthe Mart. (Amaranthaceae) (2008) Pesquisas Botânicas, 59, pp. 159-170Rozen, S., Skaletsky, H.J., Primer3 on the WWW for general users and for biologist programmers (2000) Bioinformatics, methods and protocols: Methods in molecular biology, pp. 365-386. , In, S. Krawetz and S. Misener [eds.], Humana Press, Totowa, New Jersey, USATakemoto, T., Nishimoto, N., Nakai, S., Takagi, N., Hayashi, S., Odashima, S., Wada, Y., Pfaffic acid, a novel nortriterpene from Pfaffia paniculata Kuntze (1983) Tetrahedron Letters, 24, pp. 1057-106

Adaptação, produtividade e persistência de Arachis pintoi submetido a diferentes níveis de sombreamento Adaptation, productivity and persistence of Arachis pintoi under different levels of shading

Este experimento foi realizado com o objetivo de determinar o potencial forrageiro da leguminosa Arachis pintoi, submetida a 0, 30, 50 e 70% de sombreamento, em sistemas silvipastoris e como cobertura do solo em sistemas agroflorestais. O delineamento experimental usado foi o inteiramente casualizado, com quatro repetições. Realizou-se uma avaliação no final do período chuvoso e outra no final do período seco, usando as características altura e vigor de plantas, cobertura do solo e biomassa aérea, subterrânea e total. Os resultados mostraram que A. pintoi apresentou boa adaptação e persistência nos níveis de sombreamento estudados. A produtividade, apesar de ter diminuído com o aumento dos níveis de sombreamento, foi considerada adequada mesmo nos níveis mais altos. Concluiu-se que é possível usar esta leguminosa como cobertura do solo em sistemas agroflorestais e como forrageira em sistemas silvipastoris.<br>The experiment was conducted to determine the forage potential of the Arachis pintoi submitted to 0, 30, 50 and 70% of shading, in silvopastoral systems and as ground cover in agroforestry systems. The experimental design was a completely randomized design with four replications. An evaluation was carried at the end of the rainy season and another at the end of the dry season, using the caracteristics height and plant vigor, ground cover, and total, above and below ground biomass. The results showed that A. pintoi presented good adaptation and persistence in the studied levels of shading. Although its productivity decreased with the increase of the levels of shading, it was considered adequate, even in the highest levels of shading. This indicates that it is possible to use this legume as ground cover in agroforestry systems and as forage in silvopastoral systems