Genetic Diversity And Population Structure Of The New World Screwworm Fly From The Amazon Region Of Brazil

Cochliomyia hominivorax (Coquerel) is a myiasis fly that causes economic losses to livestock farmers in warmer American regions. Previous studies of this pest had found population structure at north and south of the Amazon Basin, which was considered to be a barrier to dispersal. The present study analyzed three mitochondrial DNA (mtDNA) markers and eight nuclear microsatellite loci to investigate for the first time the genetic diversity and population structure across the Brazilian Amazon region (Amazonia). Both mtDNA and microsatellite data supported the existence of much diversity and significant population structure among nine regional populations of C. hominivorax, which was found to be surprisingly common in Amazonia. Forty-six mtDNA haplotypes were identified, of which 39 were novel and seven had previously been found only at south of Amazonia. Seventy microsatellite alleles were identified by size, moderate to high values of heterozygosity were discovered in all regions, and a Bayesian clustering analysis identified four genetic groups that were not geographically distributed. Reproductive compatibility was also investigated by laboratory crossing, but no evidence of hybrid dysgenesis was found between an Amazonian colony and one each of from Northeast and Southeast Brazil. The results have important implications for area-wide control by the Sterile Insect Technique. © 2014 International Atomic Energy Agency 2014.1382633Azeredo-Espin, A.M.L., Lessinger, A.C., Genetic approaches for studying myiasis-causing flies: molecular markers and mitochondrial genomics (2006) Genetica, 126, pp. 111-131Baumhover, A.H., Husman, C.N., Graham, A.J., Screwworms (1966) Insect Colonization and Mass Production, pp. 533-554. , Academic Press, New York, C.N. 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Perfil energético e hormonal de ovelhas Santa Inês do terço médio da gestação ao pós-parto

No período periparto ocorrem importantes adequações fisiológicas que, se não forem efetivas predispõem a fêmea a enfermidades metabólicas. O conhecimento desta adaptação é relevante para que sejam implementadas, precocemente, medidas preventivas a poupar perdas produtivas. Com este objetivo foi avaliado o perfil energético e hormonal de ovelhas Santa Inês durante a gestação e puerpério. Foram utilizadas 10 ovelhas não gestantes (G0), 10 gestantes de um (G1) e 14 gestantes de dois e três fetos (G2). Foram avaliadas concentrações plasmáticas de glicose, ácidos graxos não esterificados (AGNE), betahidroxibutirato (BHB), e as concentrações séricas de insulina, glucagon, cortisol, triiodotironina (T3) e tiroxina (T4) a partir do 88º dia de gestação até o 28º dia pós-parto. No terço final de gestação, ovelhas gestantes apresentaram maiores concentrações de AGNE, T3 e T4 que as ovelhas não gestantes. No momento do parto foram observadas maiores concentrações de glicose, AGNE e T3 para todas as ovelhas gestantes em relação às não gestantes. Não houve diferença entre as ovelhas gestantes de um, dois ou três fetos. As diferenças observadas ocorreram apenas entre ovelhas gestantes e as vazias. Portanto, quando há adequada adaptação neste período de elevado desafio metabólico, os parâmetros bioquímicos aqui considerados independem do número de fetos gestados e podem ser considerados como valores de referência para ovelhas gestantes de um feto ou mais fetos do terço médio de gestação ao primeiro mês pós-parto

Study of methodological variations in apparent nutrient metabolism determination in broiler chickens Estudo de variações metodológicas na determinação do metabolismo aparente de nutrientes em frangos de corte

An experiment was conducted to define a protocol to determine metabolizable nutrient and energy values of diets. The metabolizability (M) was calculated of the dry matter (DM); crude protein (CP); gross energy (GE) and AMEn of a single diet. Eighty-one 21-day old (d) male birds were used. The tested methodologies were: Cr2O3 (0.5%) as an indicator (partial collection) or Fe2O3 (1%) as a marker; fasting (0, 4, 6 and 8 h) prior to excreta collection and at the end of the feeding period on the last day of collection (total collection). The excreta collection periods were also tested (3 and 5 days). Twenty 31-day old male broilers from the same group of birds were used to assess the effect of fasting on digestive organ weight. At the end of fasting the digestive organs were removed and weighed. Metabolism coefficients and energy were not different between 3 and 5 days of total collection. CPM was lower for marker utilization and 3 days excreta collection compared to the total collection. Eight hours fasting resulted in significantly lower CPM compared to the other periods or the no fasting. With the methodology of partial collection with 5 days of collection, the lowest values were observed for all the responses, compared to the 3 day collection period. The use of the total collection methodology produced the highest DMM and CPM compared to partial collection. No influence of fasting was observed on the digestive organ sizes, indicating that until 8 hours of fasting no changes were observed in either relative or absolute organ weight. However, the relative jejunum weight of birds submitted to 4 hours fasting was higher than that of birds under no fasting. Total collection, during a 3 day period, without fasting and marker use, is the best methodology for ingredient and feed evaluation of growing birds.<br>Realizou-se um ensaio com frangos de corte para a definição de um protocolo de determinação da metabolizabilidade dos nutrientes e da energia da dieta. Foram estimados os coeficientes de metabolizabilidade de matéria seca (MS), proteína bruta (PB), energia bruta (EB) e a energia metabolizável aparente corrigida para nitrogênio (EMAn) de uma mesma dieta, utilizando-se 81 aves, de 21 dias de idade. As metodologias testadas foram as seguintes: utilização de 0,5% de Cr2O3 como indicador (coleta parcial) ou 1% de Fe2O3 como marcador e aplicação ou não de jejum (0, 4, 6 e 8 horas) pré-início de coleta e após a última refeição, no último dia de coleta (coletas totais). Períodos de coleta de três ou cinco dias também foram testados. Retiradas do mesmo grupo original, 20 aves de 31 dias de idade foram usadas para avaliar o efeito do jejum sobre o tamanho dos órgãos digestivos. Após o jejum, os órgãos foram retirados e pesados. Os coeficientes de metabolizabilidade e a EMAn determinados pelo método de coleta total não diferiram dos obtidos pelo método de três ou cinco dias de coleta. O uso do marcador e três dias de coleta resultou em menor metabolizabilidade da PB em relação à coleta total sem marcador. Jejum de 8 horas resultou em metabolizabilidade da PB significativamente menor em comparação aos demais períodos de jejum ou à ausência de jejum. Na coleta parcial de cinco dias, os coeficientes de metabolizabilidade foram menores para todas as respostas. Pelo método de coleta total, obtiveram-se maiores metabolizabilidades da MS e PB em comparação ao método de coleta parcial. Não foi observada influência do jejum sobre o peso absoluto ou relativo da maioria dos órgãos. No entanto, o peso relativo do jejuno das aves submetidas a 4 horas de jejum foi maior que o das aves sem jejum. A coleta total de excretas durante três dias, sem jejum e sem o uso de marcador, consiste no melhor método de avaliação de ingredientes e ração em frangos de corte em crescimento