29 research outputs found
Human Papilloma Virus: Prevalence, distribution and predictive value to lymphatic metastasis in penile carcinoma
Objectives To evaluate the prevalence, distribution and association of HPV with histological pattern of worse prognosis of penile cancer, in order to evaluate its predictive value of inguinal metastasis, as well as evaluation of other previous reported prognostic factors. Material and Methods Tumor samples of 82 patients with penile carcinoma were tested in order to establish the prevalence and distribution of genotypic HPV using PCR. HPV status was correlated to histopathological factors and the presence of inguinal mestastasis. The influence of several histological characteristics was also correlated to inguinal disease-free survival. Results Follow-up varied from 1 to 71 months (median 22 months). HPV DNA was identified in 60.9% of sample, with higher prevalence of types 11 and 6 (64% and 32%, respectively). There was no significant correlation of the histological characteristics of worse prognosis of penile cancer with HPV status. Inguinal disease-free survival in 5 years did also not show HPV status influence (p = 0.45). The only independent pathologic factors of inguinal metastasis were: stage T ≥ T1b-T4 (p = 0.02), lymphovascular invasion (p = 0.04) and infiltrative invasion (p = 0.03). conclusions HPV status and distribution had shown no correlation with worse prognosis of histological aspects, or predictive value for lymphatic metastasis in penile carcinoma
Prevalence of human papillomavirus infection and phylogenetic analysis of HPV-16 E6 variants among infected women from Northern Brazil
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Instituto Nacional de Câncer. Clinical Research Coordination. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Câncer. Clinical Research Coordination. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Background: The main cause of cervical cancer in the world is high risks human papillomavirus infection (mainlyrepresented by HPV-16 and HPV-18), that are associated to the development of malign transformation of the epithelium. HPV prevalence exhibits a wide geographical variability and HPV-16 variants have been related to an increased risk of developing cervical intraepithelial lesion. The aim of this study was to describe DNA-HPV prevalence and HPV-16 variants among a women population from Northern Brazil. Methods: One hundred and forty three women, during routine cervical cancer screening, at Juruti Project, fulfilled an epidemiological inquiry and were screened through a molecular HPV test. HPV-16 variants were determined by sequencing the HPV-16 E6 open reading frame. Results: Forty two samples were considered HPV positive (29.4 per cent). None of those had abnormal cytology results. HPV prevalence varied between different age groups (Z(U) = 14.62; p = >0.0001) and high-risk HPVs were more frequent among younger ages. The most prevalent type was HPV-16 (14 per cent) and it variants were classified, predominantly, as European (87.5 per cent). Conclusions: HPV prevalence in our population was higher than described by others and the most prevalent HPV types were high-risk HPVs. The European HPV-16 variant was the most prevalent among HPV-16 positive samples. Our study reinforces the fact that women with normal cytology and a positive molecular test for high-risk HPVs should be submitted to continuous follow up, in order to verify persistence of infection, promoting an early diagnosis of cervical cancer and/or its precursors
Detection of avian group D rotavirus using the polymerase chain reaction for VP6 gene
This study was partially supported by a grant from Pará State Secretary of Science and Technology, contract no. 067/2008 (SEDECT/FAPESPA/PA).Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Agricultura. Laboratório Nacional Agropecuário. Belém, PA, Brazil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Group D rotaviruses (RVs-D) have been documented in birds and, while they may be common in these
animals, few molecular studies are available for this specific group. In this study, specific primers for
the gene that encodes for the RVs-D VP6 protein were designed and used in a reverse transcription
polymerase chain reaction (RT-PCR). Thirty pools of samples were tested by polyacrylamide gel electrophoresis
(PAGE) yielding a 30% (9/30) positivity. These pools were subjected subsequently to RT-PCR,
with a 53% (16/30) positivity rate. The sensitivity of the PCR assay was demonstrated up to a dilution
of 5 × 10−4 ng/L (0.5 pg/L) of the cloned VP6 gene. The four samples were sequenced and showed
90.8–91.1% similarity with regards to the RVs-D VP6 gene. To assess for specificity our RT-PCR was applied
to nine samples known to contain enteric viral agents other than group D rotaviruses including picobirnavirus,
rotavirus group A, and reovirus with negative results. Overall, the data confirm the specificity of
the primers used for detecting the RVs-D by RT-PCR, suggesting that this assay can be used for diagnostic
purposes
Estudo prospectivo para avaliação da associação entre o teste molecular para HPV e o exame citológico (Papanicolau) no rastreamento do carcinoma de colo uterino
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.A relação entre a infecção por Papiloma Vírus
Humano (HPV) de alto risco oncogênico e o
câncer de colo uterino vem sendo investigada
há mais de três décadas, e hoje, depois de uma
vasta avaliação por estudos epidemiológicos e
moleculares, podemos dizer que o HPV de alto
risco oncogênico é o agente causal de tal neoplasia
maligna. Vários fatores celulares e inerentes
ao vírus podem influenciar no aparecimento e
progressão de lesões precursoras, porém o exame
mais específico para essa finalidade já possui mais
de 50 anos e é baseado em avaliação subjetiva do
examinador (Papanicolau). Alguns programas de
rastreamento para essas lesões precursoras em
países desenvolvidos usam uma associação de
metodologias moleculares e o exame citológico
(Papanicolau) para aumentar o poder preditivo
negativo, ou seja, garantir que um resultado
negativo citológico, associado ao resultado negativo
molecular, possa garantir que essa paciente não irá
desenvolver lesão de alto grau nos próximos três
anos, fazendo com que o intervalo entre os retornos
para rastreio seja maior, barateando o custo anual
por paciente avaliada nesses programas.
OBJETIVOS
Em reconhecimento a todos os avanços já citados,
e como estratégia para o rastreamento do câncer
de cérvice uterina em nossa população, propomos
a realização de um estudo prospectivo, de cinco
anos de seguimento, para o rastreamento de
lesões precursoras (NIC I, II e III) e do câncer de colo
uterino, associando o exame citológico (Papanicolau)
ao diagnóstico molecular para HPV através do
sistema de Captura Híbrida de 2ª geração.
METODOLOGIA
Este sistema utiliza sondas específicas para tipos
de alto (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58,
59 e 68) e baixo risco oncogênico (6, 11, 42, 43 e
44). Posteriormente, a avaliação de cada amostra
positiva será feita para identificação do tipo viral
específico, utilizando-se o sistema de PCR mais
Hibridação tipo específica da ROCHE, Linear Array
HPV Genotyping Test, para a identificação tipo
específica dessas infecções para cada tipo viral de
alto ou baixo risco oncogênico para que os dados
epidemiológicos possam estar mais completos.
RESULTADOS
Nas 1.394 amostras coletadas, 212 (15,2%)
foram identificadas positivas pelas metodologias
empregadas, sendo que 182 (85,8%) infecções são
por tipos de alto risco oncogênico, e 30 por tipos de
baixo risco. Em relação à citologia dessas amostras,
970 apresentaram exame citológico sem alterações,
e nestas, 159 (16,4%) eram positivas para HPV,
sendo 142 (89,3%) para alto risco e 17(10,7%) para
baixo risco.
CONCLUSÃO
Este estudo pode indicar que a maioria das
infecções nos serviços de atendimento ginecológico
públicos são por tipos de alto risco oncogênico
de HPV e que há um percentual importante das
pacientes atendidas no sistema público, com
citologia normal, porém ainda submetidas aos riscos
decorrentes da infecção por tipos de alto risco dos
Papiloma vírus, causalmente associados aos casos
de câncer de colo uterino, o que de forma geral
poderia ser detectado e mantido sob vigilância mais
específica se o teste viral fosse associado ao exame
citológico
Low frequency of human papillomavirus detection in prostate tissue from individuals from Northern Brazil
The presence of human papillomavirus (HPV) was evaluated in 65 samples of prostate tumours and six samples of prostates with benign prostatic hyperplasia from individuals from Northern Brazil. We used a highly sensitive test, the Linear Array HPV Genotyping Test, to detect 37 high and low-risk HPV types. In this study, only 3% of tumour samples showed HPV infection. Our findings support the conclusion that, despite the high incidence of HPV infection in the geographic regions studied, HPV was not associated with a higher risk of prostate cancer. To our knowledge, this is the first study evaluating the frequency of HPV detection in prostatic tissue of individuals from Brazil
Prevalence, Diversity, and Risk Factors for Cervical HPV Infection in Women Screened for Cervical Cancer in Belém, Pará, Northern Brazil
Background: Human papillomavirus (HPV) is the most common viral sexually transmitted infection of the reproductive tract, and cervical cancer is the most common HPV-related disease. This study estimated the prevalence, diversity of HPV genotypes, and associated risk factors in women screened for cervical cancer in northern Brazil. Methods: The cross-sectional study was conducted in Belém, Pará, in the Amazon region of Brazil, and it included 162 women who were spontaneously undergoing a Pap-smear routine. Epidemiological, sexual, and health-related information was collected by interviews, and cervical samples were collected for cytological examination and HPV-DNA detection. HPV genotypes were classified as low risk (LR) and high risk (HR) by nucleotide sequencing. Results: In total, 17.3% (28/162) of the participants had HPV-DNA, and LR-HPV was the most prevalent (71.4%). Among the 13 different types of HPV detected, HPV-11 was found most frequently (12/28; 42.9%), followed by HPV-31 (3/28; 10.7%). Of the participants with cytological alterations, HPV infection was detected in only four: two were diagnosed with low-grade squamous intraepithelial lesions (15.4%), one with atypical squamous cells of undetermined significance (7.7%), and one with atypical squamous cells, cannot exclude high-grade squamous intraepithelial lesions (7.7%). Of the 61 women who presented a normal cytology, 13 (21.3%) had positive tests for HPV infection, 4 (8.2%) of which were positive for a high-risk genotype. Conclusion: The prevalence of HPV was high in Belém, Pará, and especially in women who had normal cytology results, which suggests the need for greater screening for HPV infection in women’s primary health care
Whole-genome sequencing of an unusual human papillomavirus (HPV71) from Latin America (Brazil)
Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.We report the complete genome sequencing of human papillomavirus 71 from Latin America (Brazil)
Prevalence and Genotyping of HPV in Oral Squamous Cell Carcinoma in Northern Brazil
Highly oncogenic human papillomavirus (HPV) is well known to be associated with and a risk factor for various types of oral carcinomas such as oral squamous cell carcinoma (OSCC). The aim of this study was to evaluate and describe the HPV-induced OSCC prevalence and genotyping in the city of Belém, northern Brazil. This cross-sectional study features 101 participants who attended an oral pathology referral center in a dental college looking for diagnoses of oral lesions (OL). After signing the consent term and meeting the inclusion criteria, all participants went through a sociodemographic and epidemiological questionnaire. Then, OL were collected by excisional or incisional biopsy depending on OL size; after that, OL tissues were preserved in paraffin blocks to histopathological diagnoses. Afterwards, paraffin blocks were divided into benign and malignant/premalignant lesions based on the classification of potentially malignant disorders of the oral and oropharyngeal mucosa. Then, the paraffin blocks had DNA extraction performed by the ReliaPrep FFPE gDNA Miniprep method in order to identify HPV DNA of high oncogenic risk and low oncogenic risk. Then, the viral DNA was amplified and typed using the Inno-Lipa genotyping Extra II method, and the collected data were analyzed by Chi-square and G-tests. In total, 59/101 (58.4%) OL were malignant/premalignant lesions, of which OSCC was the most prevalent with 40/59 (67.7%) and 42/101 (41.6%) benign lesions. The most common area of OL incidence was upper gingiva 46/101 (45.5%). Regarding HPV DNA detection, approximately 27/101 (26.7%) had positive results; of these, 17/59 (28.8%) were malignant/premalignant lesions, and the most prevalent genotypes detected were 16, 18, 52 and 58, while among benign lesions, 10/42 (66.6%) had HPV-positive results, and the most prevalent genotypes detected were 6, 11 and 42. Age range was the only risk factor with a significant association between HPV and OSCC presence (p-value: 0.0004). A correlation between OSCC and oral HPV among analyzed samples could not be demonstrated in our small cohort