The HRA2pl fusion peptide exerts in vitro antiviral activity against human respiratory paramyxoviruses and pneumoviruses

Acute respiratory infections are a group of diseases caused by viruses, bacteria, and parasites that mainly affect children until the age of 5 and immunocompromised senior adults. In Mexico, these infections are the main cause of morbidity in children, with more than 26 million cases of respiratory infections reported by the Secretariat of Health, in 2019. The human respiratory syncytial virus (hRSV), the human metapneumovirus (hMPV), and the human parainfluenza-2 (hPIV-2) are responsible for many respiratory infections. Currently, palivizumab, a monoclonal antibody against the fusion protein F, is the treatment of choice against hRSV infections. This protein is being studied for the design of antiviral peptides that act by inhibiting the fusion of the virus and the host cell. Therefore, we examined the antiviral activity of the HRA2pl peptide, which competes the heptad repeat A domain of the F protein of hMPV. The recombinant peptide was obtained using a viral transient expression system. The effect of the fusion peptide was evaluated with an in vitro entry assay. Moreover, the effectiveness of HRA2pl was examined in viral isolates from clinical samples obtained from patients with infections caused by hRSV, hMPV, or hPIV-2, by evaluating the viral titer and the syncytium size. The HRA2pl peptide affected the viruses’ capacity of entry, resulting in a 4-log decrease in the viral titer compared to the untreated viral strains. Additionally, a 50% reduction in the size of the syncytium was found. These results demonstrate the antiviral potential of HRA2pl in clinical samples, paving the way toward clinical trials

Aplicación de un programa de madurez vocacional en estudiantes de secundaria víctimas de marginación social

The present research work, "Program" I decide my future "in vocational maturity, in students who are victims of social marginalization of the 4th grade of the advanced cycle of CEBA Herman Busse de la Guerra, 2095, 2018.", sought to know the effects it produces the intervention of a vocational guidance plan, made up of workshops based on psychopedagogical principles, and whose didactic application is based on the objectives and principles of the proposal. The research was of an applied type, of a pre-experimental design. The study group consisted of 84 students, distributed in 42 in the experimental group and 42 in the control group. The pre-test was applied using the emotional maturity questionnaire where scores were obtained in the emotional maturity dimension (t = 1.21, p = 0.23), in the planning dimension (t = 0.62, p = 0.54), the exploration dimension (t = 1.11, p = 0.27), the information dimension (t = 1.11, p = 0.27) and finally in the decision-making dimension (U = 844, p = 0.73) showing that there are no statistically significant differences in the pretest in the experimental group and control group. The post-test results show us that after having applied the vocational program, the control and experimental groups did show significant differences in its five dimensions: In emotional maturity (t = 9.28, p = 0.00), in the planning dimension (t = 0.62, p = .001), in the exploration dimension (t = 4.865, p = 0.000), in the information dimension (U = 314.5, p = 0.000), and in the decision-making dimension (U = 71.00, p = 0.000). The study verified the effectiveness of the program "I decide my future"El presente trabajo de investigación, “Programa “Decido mi futuro” en la madurez vocacional, en estudiantes víctimas de marginación social del 4to grado del ciclo avanzado del CEBA Herman Busse de la Guerra, 2095, 2018.”, buscó conocer los efectos que produce la intervención de un plan de orientación vocacional, conformado por talleres fundamentados en principios psicopedagógicos, y cuya aplicación didáctica está en función de los objetivos y principios de la propuesta. La investigación fue de tipo aplicada, de diseño pre experimental. El grupo de estudio estuvo conformado por 84 estudiantes, distribuidos en 42 en el grupo experimental y 42 en el grupo control. Se aplicó el pretest utilizando el cuestionario de madurez emocional donde se obtuvieron puntajes en dimensión madurez emocional (t=1.21, p=0.23), en la dimensión planificación (t = 0.62, p = 0.54), la dimensión exploración (t = 1.11, p = 0.27), la dimensión información (t = 1.11, p = 0.27) y por último en la dimensión toma de decisiones (U = 844, p = 0.73) evidenciándose que no existen diferencias estadísticamente significativas en el pretest en el grupo experimental y grupo control. Los resultados del postest nos muestran que después de haber aplicado el programa vocacional los grupos control y experimental si se evidenciaron diferencias significativas en sus cinco dimensiones: En madurez emocional (t = 9.28, p = 0.00), en la dimensión planificación (t = 0.62, p = .001), en la dimensión exploración (t = 4.865, p = 0.000), en la dimensión información (U = 314.5, p = 0.000), y en la dimensión toma de decisiones (U = 71.00, p = 0.000). El estudio comprobó la eficacia del programa “Decido mi futuro”