A note on the use of a lumbar joint as a predictor of body fat depots in Aragonesa ewes with different body confition scores

The lumbar joint, which is handled to assess body condition scores, was taken from 52 adult Rasa Aragonesa ewes with body condition scores between 1·5 and 4·5 and dissected into muscle, bone, subcutaneous and intermuscular fat. The subcutaneous fat in the lumbar joint was highly correlated with total fat in the body (r=0·97), confirming the value of this region for assessing body condition in Rasa Aragonesa ewes

Relationships between fat depots and body condition score or tail fatness in Rasa Aragonesa breed

The relationships between body fat depots and body condition score (BCS) were determined in 52 adult Rasa Aragonesa ewes aged 10 (s.d. 2) years and ranging in BCS from 1-5 to 4•5. BCS of each ewe was assessed by three people. the repeatability within individual being 90"/0 and between individuals 80%. The ewes were weighed before slaughter. After slaughter the omental mesenteric kidney and pelvic fat were separated and weighed. The fat of the left side of the carcass was separated into subcutaneous and intermuscular depots. The relationship between live weight and BCS was semilogarithmic and those between fat depots and BCS were logarithmic. Regression analysis was also used to describe the relationships between the various fat depots and BCS or live weight Of the variation in total fat weight. proportionately 0•90 was accounted for by variations in BCS while 0•84 was accounted for by variations in live weight. For individual fat depots proportionately 0•86 to 0•90 of the variation was accounted for by variation in BCS and 0•69 to 0-79 by variation in live weight. BCS was a better predictor than live weight of the weight of both total body fat and the individual fat depots. A curvilinear regression between BCS and live weight showed that the increases in live weight for a unit change in BCS was 7, 10, 12 and 16 kg for each one point increase ill BCS from 1 to 5 respectively. The tail fat depot (tail fatness score) •Was assessed in the same ewes by score on a three-point scale. Of the variation in the weight of individual fat depots proportionately 0•79 to 0•86 was accounted for by variation in tail fatness score. Thus the tail fatness score could be used as an additional method of assessing body condition in the Aragonesa breed

Conformación, engrasamiento y sistemas de clasificación de la canal caprina

España es el segundo país productor de ganado caprino de In UE, con un 21 % del censo total, tras Grecia que posee el 48,7% (Delfa, 2004). A pesar de ello no existe en nuestro país ningún distintivo o Marca de Calidad para la canal y carne caprina, mientras que en Portugal, con un censo del 27,8% del español y una producción de carne que tan solo supone el 13,9% de la española, tiene cinco Indicaciones Geográficas Protegidas. Existe por lo tanto un gran desconocimiento sobre las razas caprinas autóctonas españolas, que se acrecienta con las de aptitud cárnica. A este respecto, Devendra (2000) y Lebbie (2000) indicaban la existencia de importantes lagunas en el conocimiento de esta especie, reconociendo recientementc Morand-Fehr y Lebbie (2004) la necesidad de analizar su situación actual con el fin de prepararla para los retos de un próximo futuro

Ultrasonic estimates of fat thickness, C measurement and longissimus dorsi depth in rasa aragonesa ewes with same body condition score

Ultrasonic estimates of fat thickness, C measurement and longissimus dorsi depth were assessed in 14 Rasa Aragonesa ewes with same body condition score. The ultrasonic estimates have been used for predicting tota1 carcass fat and muscle. The 76% of the variation in carcass lumbar fat thickness was accounted for by variation in lumbar fat thickness assessed by ultrasonic machine, whereas 53% of the variation in C measurement was accounted. Nevertheless only 5% of variation in m. Longissimus dorsi depth was accounted for by variation in this measurement assessed by ultrasonics. The inclusion of cold carcass weight as an independent variable in a multiple regression with lumbar fat thickness assessed by ultrasonics and the same measurement obtained on carcass improve the precision of total carcass fat and muscle predictions (r=81 and 91%)

Salicylic Acid, an Ambimobile Molecule Exhibiting a High Ability to Accumulate in the Phloem

International audienceThe ability of exogenous salicylic acid (SA) to accumulate in castor bean (Ricinus communis) phloem was evaluated by HPLC and liquid scintillation spectrometry analyses of phloem sap collected from the severed apical part of seedlings. Time-course experiments indicated that SA was transported to the root system via the phloem and redistributed upward in small amounts via the xylem. This helps to explain the peculiarities of SA distribution within the plant in response to biotic stress and exogenous SA application. Phloem loading of SA at 1, 10, or 100 mM was dependent on the pH of the cotyledon incubating solution, and accumulation in the phloem sap was the highest (about 10-fold) at the most acidic pH values tested (pH 4.6 and 5.0). As in animal cells, SA uptake still occurred at pH values close to neutrality (i.e. when SA is only in its dissociated form according to the calculations made by ACD LogD suite software). The analog 3,5-dichlorosalicylic acid, which is predicted to be nonmobile according to the models of Bromilow and Kleier, also moved in the sieve tubes. These discrepancies and other data may give rise to the hypothesis of a possible involvement of a pH-dependent carrier system translocating aromatic monocarboxylic acids in addition to the ion-trap mechanism

Members of the Hyposoter didymator Ichnovirus repeat element gene family are differentially expressed in Spodoptera frugiperda

BACKGROUND: The abundance and the conservation of the repeated element (rep) genes in Ichnoviruses genomes suggest that this gene family plays an important role in viral cycles. In the Ichnovirus associated with the wasp Hyposoter didymator, named HdIV, 10 rep genes were identified to date. In this work, we report a relative quantitative transcription study of these HdIV rep genes in several tissues of the lepidopteran host Spodoptera frugiperda as well as in the H. didymator wasps. RESULTS: The data obtained in this work indicate that, in the early phases of infection (24 hours), HdIV rep genes each display different levels of transcripts in parasitized 2(nd )instar or HdIV-injected last instar S. frugiperda larvae. Only one, rep1, is significantly transcribed in female wasps. Transcript levels of the HdIV rep genes were found as not correlated to their copy number in HdIV genome. Our results also show that HdIV rep genes display different tissue specificity, and that they are primarily transcribed in S. frugiperda fat body and cuticular epithelium. CONCLUSION: This work is the first quantitative analysis of transcription of the ichnovirus rep gene family, and the first investigation on a correlation between transcript levels and gene copy numbers in Ichnoviruses. Our data indicate that, despite similar gene copy numbers, not all the members of this gene family are significantly transcribed 24 hours after infection in lepidopteran larvae. Additionally, our data show that, as opposed to other described HdIV genes, rep genes are little transcribed in hemocytes, thus suggesting that they are not directly associated with the disruption of the immune response but rather involved in other physiological alterations of the infected lepidopteran larva

Supergravity based inflation models: a review

In this review, we discuss inflation models based on supergravity. After explaining the difficulties in realizing inflation in the context of supergravity, we show how to evade such difficulties. Depending on types of inflation, we give concrete examples, particularly paying attention to chaotic inflation because the ongoing experiments like Planck might detect the tensor perturbations in near future. We also discuss inflation models in Jordan frame supergravity, motivated by Higgs inflation.Comment: 30 pages, invited review for Classical and Quantum Gravity, published versio

Design and Calibration of Moisture Sensor Based on Electromagnetic Field Measurement for Irrigation Monitoring

[EN] Soil moisture control is crucial to assess irrigation efficiency in green areas and agriculture. In this paper, we propose the design and calibration of a sensor based on inductive coils and electromagnetic fields. The proposed prototypes should meet a series of requirements such as low power consumption, low relative error, and a high voltage difference between the minimum and maximum moisture. We tested different prototypes based on two copper coils divided into two different sets (P1-P15 and NP1-NP4). The prototypes have different characteristics: variations in the number and distribution of spires, existence or absence of casing, and copper wires with a diameter of 0.4 or 0.6 mm. In the first set of experiments carried out in commercial soil, the results showed that the best prototypes were P5, P8, and P9. These prototypes were used in different types of soils, and P8 was selected for the subsequent tests. We carried the second set of experiments using soil from an agricultural field. Based on the data gathered, mathematical models for the calibration of prototypes were obtained and verified. In some cases, two equations were used for different moisture intervals in a single prototype. According to the verification results, NP2 is the best prototype for monitoring the moisture in agricultural lands. It presented a difference in induced voltage of 1.8 V, at 500 kHz, between wet and dry soil with a maximum voltage of 5.12 V. The verification of the calibration determined that the calibration using two mathematical models offers better results, with an average absolute error of 2.1% of moisture.This work is funded by the European Union under ERANETMED (Euro-Mediterranean Cooperation through ERANET joint activities and beyond), project ERANETMED3-227 SMARTWATIR and the European Union, MAPA and Comunidad de Madrid (through IMIDRA), under the project PDR18-XEROCESPED of the PDR-CM 2014-2020 (operative programme of the European Agriculture Fund for Rural Development, EAFRD). L.P. is funded by Conselleria de Educacion, Cultura y Deporte, programme Subvenciones para la contratacion de personal investigador en fase postdoctoral, grant APOSTD/2019/04; J.R. by the Ministerio de Educacion, Cultura y Deporte, through the "Ayudas para contratacion pre-doctoral de Formacion del Profesorado Universitario FPU (Convocatoria 2016)" grant number FPU16/05540; and M.P. by the Universitat Politecnica de Valencia through the pre-doctoral PAID-01-20 programme.Basterrechea-Chertudi, DA.; Rocher-Morant, J.; Parra-Boronat, M.; Parra-Boronat, L.; Marín, JF.; Mauri, PV.; Lloret, J. (2021). Design and Calibration of Moisture Sensor Based on Electromagnetic Field Measurement for Irrigation Monitoring. Chemosensors. 9(9):1-32. https://doi.org/10.3390/chemosensors9090251S1329

Gene expression profiling of Spodoptera frugiperda hemocytes and fat body using cDNA microarray reveals polydnavirus-associated variations in lepidopteran host genes transcript levels

BACKGROUND: Genomic approaches provide unique opportunities to study interactions of insects with their pathogens. We developed a cDNA microarray to analyze the gene transcription profile of the lepidopteran pest Spodoptera frugiperda in response to injection of the polydnavirus HdIV associated with the ichneumonid wasp Hyposoter didymator. Polydnaviruses are associated with parasitic ichneumonoid wasps and are required for their development within the lepidopteran host, in which they act as potent immunosuppressive pathogens. In this study, we analyzed transcriptional variations in the two main effectors of the insect immune response, the hemocytes and the fat body, after injection of filter-purified HdIV. RESULTS: Results show that 24 hours post-injection, about 4% of the 1750 arrayed host genes display changes in their transcript levels with a large proportion (76%) showing a decrease. As a comparison, in S. frugiperda fat body, after injection of the pathogenic JcDNV densovirus, 8 genes display significant changes in their transcript level. They differ from the 7 affected by HdIV and, as opposed to HdIV injection, are all up-regulated. Interestingly, several of the genes that are modulated by HdIV injection have been shown to be involved in lepidopteran innate immunity. Levels of transcripts related to calreticulin, prophenoloxidase-activating enzyme, immulectin-2 and a novel lepidopteran scavenger receptor are decreased in hemocytes of HdIV-injected caterpillars. This was confirmed by quantitative RT-PCR analysis but not observed after injection of heat-inactivated HdIV. Conversely, an increased level of transcripts was found for a galactose-binding lectin and, surprisingly, for the prophenoloxidase subunits. The results obtained suggest that HdIV injection affects transcript levels of genes encoding different components of the host immune response (non-self recognition, humoral and cellular responses). CONCLUSION: This analysis of the host-polydnavirus interactions by a microarray approach indicates that the presence of HdIV induces, directly or indirectly, variations in transcript levels of specific host genes, changes that could be responsible in part for the alterations observed in the parasitized host physiology. Development of such global approaches will allow a better understanding of the strategies employed by parasites to manipulate their host physiology, and will permit the identification of potential targets of the immunosuppressive polydnaviruses