Feeding Electrograms and Salivary Fluid Secretion of Hard Ticks (Acari: Ixodidae)

Entomolog

Ly-alpha Emission-Line Galaxies at z = 3.1 in the Extended Chandra Deep Field South

We describe the results of an extremely deep, 0.28 deg^2 survey for z = 3.1 Ly-alpha emission-line galaxies in the Extended Chandra Deep Field South. By using a narrow-band 5000 Anstrom filter and complementary broadband photometry from the MUSYC survey, we identify a statistically complete sample of 162 galaxies with monochromatic fluxes brighter than 1.5 x 10^-17 ergs cm^-2 s^-1 and observers frame equivalent widths greater than 80 Angstroms. We show that the equivalent width distribution of these objects follows an exponential with a rest-frame scale length of w_0 = 76 +/- 10 Angstroms. In addition, we show that in the emission line, the luminosity function of Ly-alpha galaxies has a faint-end power-law slope of alpha = -1.49 +/- 0.4, a bright-end cutoff of log L^* = 42.64 +/- 0.2, and a space density above our detection thresholds of 1.46 +/- 0.12 x 10^-3 h70^3 galaxies Mpc^-3. Finally, by comparing the emission-line and continuum properties of the LAEs, we show that the star-formation rates derived from Ly-alpha are ~3 times lower than those inferred from the rest-frame UV continuum. We use this offset to deduce the existence of a small amount of internal extinction within the host galaxies. This extinction, coupled with the lack of extremely-high equivalent width emitters, argues that these galaxies are not primordial Pop III objects, though they are young and relatively chemically unevolved.Comment: 45 pages, 15 figures, accepted for publication in the Astrophysical Journa

Stretch Activated Channels in Proprioceptive Organs of Crab and Crayfish Are Sensitive to Gadolinium but not Amiloride, Ruthenium Red or Low pH

The type of stretch activated receptors (SARs) in the chordotonal organs in the crab walking leg and of the muscle receptor organ (MRO) in the crayfish abdomen have not yet been classified as to their molecular or pharmacological profile. The purpose of this study is to examine the pharmacological profile of SARs in the proprioceptive neurons in the crab and crayfish models. Since many SARs share the pharmacological profile of displaying low pH or being proton sensitive (i.e. being more active) or blocked by the diuretic amiloride or ruthenium red as well as being blocked by the broad stretch activated channel blocker gadolinium (Gd3+), we used these agents to screen the receptors. Various displacement rates as well as static positions that activate the stretch activated receptors were used in examining their pharmacological profiles. Hour-long exposure to low pH decreased neural activity of the chordotonal organ of the crab more so than to amiloride or ruthenium red. The crayfish MRO did not show pH sensitivity or sensitivity to amiloride or ruthenium red. Gd3+ rapidly blocked neural activity in both the crab and crayfish. It appears these stretch activated receptors may not have a classification that is suited to the standard pharmacological profiles. The molecular makeup of the channels also awaits characterization. This could reveal a novel SAR subtype. Our neurophysiology course1 took this project on as a course-based undergraduate research experience (CURE) to address an authentic research question

The Effect of CO\u3csub\u3e2\u3c/sub\u3e, Intracellular pH and Extracellular pH on Mechanosensory Proprioceptor Responses in Crayfish and Crab

Proprioceptive neurons monitor the movements of limbs and joints to transduce the movements into electrical signals. These neurons function similarly in species from arthropods to humans. These neurons can be compromised in disease states and in adverse environmental conditions such as with changes in external and internal pH. We used two model preparations (the crayfish muscle receptor organ and a chordotonal organ in the limb of a crab) to characterize the responses of these proprioceptors to external and internal pH changes as well as raised CO2. The results demonstrate the proprioceptive organs are not highly sensitive to changes in extracellular pH, when reduced to 5.0 from 7.4. However, if intracellular pH is decreased by exposure to propionic acid or saline containing CO2, there is a rapid decrease in firing rate in response to joint movements. The responses recover quickly upon reintroduction of normal pH (7.4) or saline not tainted with CO2. These basic understandings may help to address the mechanistic properties of mechanosensitive receptors in other organisms, such as muscle spindles in skeletal muscles of mammals and tactile as well as pressure (i.e., blood pressure) sensory receptors

The Physical Nature of Lyman Alpha Emitting Galaxies at z=3.1

We selected 40 candidate Lyman Alpha Emitting galaxies (LAEs) at z ~=3.1 with observed frame equivalent widths >150A and inferred emission line fluxes >2.5x10^-17 ergs/cm^2/s from deep narrow-band and broad-band MUSYC images of the Extended Chandra Deep Field South. Covering 992 sq. arcmin, this is the largest ``blank field'' surveyed for LAEs at z ~3, allowing an improved estimate of the space density of this population of 3+-1x10^-4 h_70^3/Mpc^3. Spectroscopic follow-up of 23 candidates yielded 18 redshifts, all at z ~=3.1. Over 80% of the LAEs are dimmer in continuum magnitude than the typical Lyman break galaxy spectroscopic limit of R= 25.5 (AB), with a median continuum magnitude R ~=27 and very blue continuum colors, (V-z) ~=0. Over 80% of the LAEs have the right UVR colors to be selected as Lyman break galaxies, but only 10% also have R<=25.5. Stacking the UBVRIzJK fluxes reveals that LAEs have stellar masses ~=5x10^8 h_70^-2 M_sun and minimal dust extinction, A_V < ~ 0.1. Inferred star formation rates are ~=6 h_70^-2 M_sun/yr, yielding a cosmic star formation rate density of 2x10^-3 h_70 M_sun/yr/Mpc^3. None of our LAE candidates show evidence for rest-frame emission line equivalent widths EW_rest>240A which might imply a non-standard IMF. One candidate is detected by Chandra, implying an AGN fraction of 2+-2% for LAE candidate samples. In summary, LAEs at z ~ 3 have rapid star formation, low stellar mass, little dust obscuration and no evidence for a substantial AGN component.Comment: ApJ Letters, in press, very minor revisions to match accepted version, 4 pages with 2 color figure

Improving Student Success: Researching How Students Use Electronic Library Resources

This presentation presents a research study conducted at UNT. The UNT Libraries partnered with the Department of Anthropology at UNT to conduct ethnographic research of how UNT students use the electronic library services

EMG-Normalised Kinase Activation during Exercise Is Higher in Human Gastrocnemius Compared to Soleus Muscle

In mice, certain proteins show a highly confined expression in specific muscle groups. Also, resting and exercise/contraction-induced phosphorylation responses are higher in rat skeletal muscle with low mitochondrial content compared to muscles with high mitochondrial content, possibly related to differential reactive oxygen species (ROS)-scavenging ability or resting glycogen content. To evaluate these parameters in humans, biopsies from soleus, gastrocnemius and vastus lateralis muscles were taken before and after a 45 min inclined (15%) walking exercise bout at 69% VO2max aimed at simultaneously activating soleus and gastrocnemius in a comparable dynamic work-pattern. Hexokinase II and GLUT4 were 46–59% and 26–38% higher (p<0.05) in soleus compared to the two other muscles. The type I muscle fiber percentage was highest in soleus and lowest in vastus lateralis. No differences were found in protein expression of signalling proteins (AMPK subunits, eEF2, ERK1/2, TBC1D1 and 4), mitochondrial markers (F1 ATPase and COX1) or ROS-handling enzymes (SOD2 and catalase). Gastrocnemius was less active than soleus measured as EMG signal and glycogen use yet gastrocnemius displayed larger increases than soleus in phosphorylation of AMPK Thr172, eEF2 Thr56 and ERK 1/2 Thr202/Tyr204 when normalised to the mean relative EMG-signal. In conclusion, proteins with muscle-group restricted expression in mice do not show this pattern in human lower extremity muscle groups. Nonetheless the phosphorylation-response is greater for a number of kinase signalling pathways in human gastrocnemius than soleus at a given activation-intensity. This may be due to the combined subtle effects of a higher type I muscle fiber content and higher training status in soleus compared to gastrocnemius muscle

The development of a HAMstring InjuRy (HAMIR) index to mitigate injury risk through innovative imaging, biomechanics, and data analytics : Protocol for an observational cohort study

Background The etiology of hamstring strain injury (HSI) in American football is multi-factorial and understanding these risk factors is paramount to developing predictive models and guiding prevention and rehabilitation strategies. Many player-games are lost due to the lack of a clear understanding of risk factors and the absence of effective methods to minimize re-injury. This paper describes the protocol that will be followed to develop the HAMstring InjuRy (HAMIR) index risk prediction models for HSI and re-injury based on morphological, architectural, biomechanical and clinical factors in National Collegiate Athletic Association Division I collegiate football players. Methods A 3-year, prospective study will be conducted involving collegiate football student-athletes at four institutions. Enrolled participants will complete preseason assessments of eccentric hamstring strength, on-field sprinting biomechanics and muscle–tendon volumes using magnetic-resonance imaging (MRI). Athletic trainers will monitor injuries and exposure for the duration of the study. Participants who sustain an HSI will undergo a clinical assessment at the time of injury along with MRI examinations. Following completion of structured rehabilitation and return to unrestricted sport participation, clinical assessments, MRI examinations and sprinting biomechanics will be repeated. Injury recurrence will be monitored through a 6-month follow-up period. HAMIR index prediction models for index HSI injury and re-injury will be constructed. Discussion The most appropriate strategies for reducing risk of HSI are likely multi-factorial and depend on risk factors unique to each athlete. This study will be the largest-of-its-kind (1200 player-years) to gather detailed information on index and recurrent HSI, and will be the first study to simultaneously investigate the effect of morphological, biomechanical and clinical variables on risk of HSI in collegiate football athletes. The quantitative HAMIR index will be formulated to identify an athlete’s propensity for HSI, and more importantly, identify targets for injury mitigation, thereby reducing the global burden of HSI in high-level American football players. Trial Registration The trial is prospectively registered on ClinicalTrials.gov (NCT05343052; April 22, 2022)