Rock mass characterization coupled with seismic noise measurements to analyze the unstable cliff slope of the Selmun Promontory (Malta)

In the Mediterranean area, cliff slopes represent widespread high-risk landforms as they are highly frequented touristic places often interested by landslide processes. Malta represents a significant case study as several cliffs located all around the island are involved in instability processes, as evidenced by wide block-size talus distributed all along the coast line. These diffused instabilities are related to the predisponding geological setting of Malta Island, i.e. the over-position of grained limestone on plastic clay deposits, that induces lateral spreading phenomena associated to falls and topples of different-size rock blocks and is responsible for a typical landscape with stable plateau of stiff rocks bordered by unstable cliff slopes. The ruins of Gƫajn ƪadid Tower, the first of the thirteen watchtowers built in 1658 by the Gran Master Martin de Redin, stand out in the Selmun area. Currently the safety of this important heritage site, already damaged by an earthquake on October 12th 1856, is threaten by a progressive moving of the landslide process towards the stable plateau area. During autumn 2015, a field campaign was realized to characterize the jointed rock mass. A detailed engineering-geological survey was carried out to reconstruct the geological setting and to define the mechanical properties of the rock mass. Based on the surveyed joint spatial distribution, 58 single-station noise measurements were deployed to cover both the unstable zone and the stable area. The obtained 1-hour records were analyzed in the frequency domain for associating vibrational evidences to different instability levels, i.e. deriving the presence of already isolated blocks by the local seismic response. The here presented results can be a useful contribute to begin to asses defense strategies for the Selmun Promontory, in the frame of managing the landslide risk in the study area and preserving the local historical heritage

Photoluminescence dispersion as a probe of structural inhomogeneity in silica

We report time-resolved photoluminescence spectra of point defects in amorphous silicon dioxide (silica), in particular the decay kinetics of the emission signals of extrinsic Oxygen Deficient Centres of the second type from singlet and directly-excited triplet states are measured and used as a probe of structural inhomogeneity. Luminescence activity in sapphire ($\alpha$-Al$_2$O$_3$) is studied as well and used as a model system to compare the optical properties of defects in silica with those of defects embedded in a crystalline matrix. Only for defects in silica, we observe a variation of the decay lifetimes with emission energy and a time dependence of the first moment of the emission bands. These features are analyzed within a theoretical model with explicit hypothesis about the effect introduced by the disorder of vitreous systems. Separate estimations of the homogenous and inhomogeneous contributions to the measured emission linewidth are obtained: it is found that inhomogeneous effects strongly condition both the triplet and singlet luminescence activities of oxygen deficient centres in silica, although the degree of inhomogeneity of the triplet emission turns out to be lower than that of the singlet emission. Inhomogeneous effects appear to be negligible in sapphire

Cancer stem-neuroendocrine cells in an atypical carcinoid case report.

Lung neuroendocrine cells tumor (NET) classification and diagnosis, particularly for typical and atypical carcinoids, are complicated by a variable natural history and nonspecific symptoms. Mechanisms for the development and progression of well-differentiated lung NETs are still unclear. An accurate and timely diagnosis can ensure the implementation of appropriate treatment and impact on prognosis. One of the main unclear point is the definition of these cells’ composition. In fact, it is known that carcinoids are mainly constituted by neuroendocrine cells. Aim of our report is to show for the first time the presence of a high percentage of cancer stem cells (CSCs) in an atypical carcinoid. The ALDEFLUOR assay was used to identify and sort ALDHhigh and ALDHlow human lung cancer cells following tissue digestion. SOX2 was additionally determined by immunohistochemistry. All specimens contained the 53.10% of ALDHhigh cells among all viable lung cancer cells, which indicates that more than half of the entire tumor cell population was composed by CSCs. As expected also in immunohistochemistry, about a half of the nuclei of the cells were positive for SOX2. We strongly support the hypothesis of the presence of cancer stem-neuroendocrine cells (CSCs-NETs) as subpopulation in these types of tumors

Cancer stem cells and their microenvironment.

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Evaluating characteristics of an active coastal spreading area combining geophysical data with satellite, aerial, and unmanned aerial vehicles images

The northern region of the Maltese archipelago is experiencing lateral spreading landslide processes. This region is characterized by cliffs with a hard coralline limestone outcropping layer sitting on a thick layer of clay. Such a geological configuration causes coastal instability that results in lateral spreading which predispose to rockfalls and topplings all over the cliff slopes. The aim of this research was to develop a methodology for evaluating cliff erosion/retreat using the integration of geomatics and geophysical techniques. Starting from a 3D digital model of the Selmun promontory, generated by unmanned aerial vehicle (UAV) photogrammetry, it was possible to map the fractures and conduct geophysical measurements such as electrical resistivity tomography and ground penetrating radar for the identification and mapping of vertical fractures affecting the hard coralline limestone plateau, and to create a 3D geological model of the study area. In addition to this, high-accuracy orthophotos from UAV that were captured between 1957 and 2021 were georeferenced into a GIS and compared to aerial and satellite images. The movement and evolution of boulders and cracks in rocks were then vectorized to highlight, track and quantify the phenomenon through time. The results were used to derive a qualitative assessment of the coastal variations in the geometric properties of the exposed discontinuity surfaces to evaluate the volumes and the stop points of the observed rockfalls. The outcomes of this research were finally imported in a GIS which offers an easy approach for the collection and processing of coastal monitoring data. In principle, such a system could help local authorities to address social, economic and environmental issues of pressing importance as well as facilitate effective planning in view of a risk mitigation strategy

Cancer stem cells and macrophages: molecular connections and future perspectives against cancer.

Cancer stem cells (CSCs) have been considered the key drivers of cancer initiation and progression due to their unlimited self-renewal capacity and their ability to induce tumor formation. Macrophages, particularly tumor-associated macrophages (TAMs), establish a tumor microenvironment to protect and induce CSCs development and dissemination. Many studies in the past decade have been performed to understand the molecular mediators of CSCs and TAMs, and several studies have elucidated the complex crosstalk that occurs between these two cell types. The aim of this review is to define the complex crosstalk between these two cell types and to highlight potential future anti-cancer strategies

Early inspiratory effort assessment by esophageal manometry early predicts noninvasive ventilation outcome in de novo respiratory failure: a pilot study.

Rationale: The role of inspiratory effort has still to be determined as a potential predictors of non-invasive mechanical ventilation (NIV) failure in acute hypoxic de novo respiratory failure (AHRF). Objectives: We explore the hypothesis that inspiratory effort might be a major determinant of NIV failure in these patients. Methods: Thirty consecutive patients with AHRF admitted to a single center and candidates for a 24-hour NIV trial were enrolled. Clinical features, tidal changes in esophageal (ΔPes) and dynamic transpulmonary pressure (ΔPL), expiratory tidal volume, and respiratory rate were recorded on admission and 2-4-12-24 hours after NIV start, and were tested for correlation with outcomes. Measurements and Main Results: ΔPes and ΔPes/ΔPL were significantly lower 2 hours after NIV start in patients who successfully completed the NIV trial (n=18) compared to those who needed endotracheal intubation (n=12) [median=11 (IQR=8–15) cmH2O vs 31.5 (30–36) cmH2O, p<0.0001] while other variables differed later. ΔPes was not related to other predictors of NIV failure at baseline. NIV-induced reduction in ΔPes of 10 cmH2O or more after 2 hours of treatment was strongly associated to avoidance of intubation, and represented the most accurate predictor of treatment success (OR=15, 95%CI 2.8-110, p=0.001, AUC=0.97, 95%CI 0.91–1, p<0.0001). Conclusions: The magnitude of inspiratory effort relief as assessed by ΔPes variation within the first 2 hours of NIV was an early and accurate predictor of NIV outcome at 24 hours

Expression of ALDH and SOX-2 in Pulmonary Sclerosing Pnemocytoma (PSP) of the Lung: Is There a Meaning Behind?

Background: Pulmonary sclerosing pneumocytoma (PSP) is a rare benign pulmonary tumor that derives from primitive respiratory epithelium of the pulmonary alveolus. The etiology and pathogenesis are still unclear. Histopathological diagnosis focuses on cells that are positive for TTF1, EMA, cytokeratin-7, and CAM 5.2. The aim of our study is to highlight the elevated expression of ALDH and the presence of SOX-2 in pulmonary sclerosing pneumocytoma. Methods: We report five cases of pulmonary sclerosing pneumocytoma undergone surgery at our Division of Thoracic Surgery, during a period between 1994 and 2011. ALDH and SOX-2 markers were also tested for positivity in all the patients. Results: Patients showed elevated expression of ALDH during immunohistochemistry and mild expression of SOX-2, although in two cases in which SOX-2 was highly expressed. Among these two patients, one presented with lymph node recurrence while the other had no recurrence with a PET-positive nodule. In particular, the patient who had developed recurrence had an ALDH score of 4 and a SOX-2 score of 3, whereas the patient with the PET-positive nodule showed an ALDH score of 4 with a mild SOX-2expression of score 1. Conclusions: This is the first attempt demonstrating the elevated expression of ALDH in this disease. SOX-2 expression was noted in both the patient who developed recurrence and the patient with a PET-positive nodule. We believe that further investigation may be highly useful to better characterize these two markers as well as understandtheir function

GREASE II. A phase II randomized, 12-month, parallel-group, superiority study to evaluate the efficacy of a Modified Atkins Diet in Autosomal Dominant Polycystic Kidney Disease patients

Introduction Autosomal Dominant Polycystic Kidney Disease (ADPKD) is a condition that causes progressive renal function decline. Preclinical data suggest the presence of a profound metabolic derangement in ADPKD. Cystic cells shift their energy metabolism from oxidative phosphorylation to aerobic glycolysis, show inhibition of fatty acid oxidation and become glutamine and arginine dependent. Recent preclinical experiences have suggested beneficial effect in terms of reduction of cystic size, interstitial fibrosis and disease progression, targeting these deregulated metabolic pathways by ketosis induction. The dietetic approach to ADPKD, because of low cost and absence of toxicity, represents an interesting therapeutic strategy. Methods and analysis The protocol describes a phase II clinical trial that will evaluate the effect on Total Kidney Volume, safety and tolerability of a ketogenic diet in a selected ADPKD population. The trial will have, as secondary objective, the evaluation of the ability of the ketogenic diet to slow down the renal function decline. This will be a 12-month randomized, parallel group, two arm, superiority trial with 1:1 allocation to evaluate the efficacy of a Modified Atkins Diet protocol compared to a balanced normocaloric diet on 90 ADPKD patients. Dissemination The study results will be released to the patients and the medical community