A cleavage map of bacteriophage Phi-X174 genome

Restriction endonucleases isolated from Hemophilus influenzae, Hemophilus parainfluenzae, and Hemophilus aegyptius were used to cleave phi-X174 replicative form DNA into three sets of specific DNA fragments. The order of these fragments in the phi-X replicative form molecule was determined by (1) analysis of partial digest products, (2) analysis of overlapping sets of fragments produced by two different restrictive enzymes. On the basis of these results, a detailed physical map of the phi-X174 genome has been constructed with respect to the cleavage sites of all three enzymes

Collider Signatures from the Brane World

We discuss some collider signatures of the brane world. In addition to the usual bulk (closed string) fields and brane (open string) fields in the Type I string picture, there are closed string fields, namely, twisted modes, which are not confined on the branes but nonetheless are localized in the extra compactified dimensions. While the coupling of the Standard Model (brane) fields with a bulk mode (such as the graviton) is suppressed by powers of the Planck mass, their coupling to a twisted mode is suppressed only by powers of the string scale M_s, which can be as low as a few TeV. This means these localized twisted fields can have important observable effects in the TeV range, including resonances in dijet invariant mass distributions in \bar p p, pp \to jets + X. Given the current lower bound on the fundamental higher-dimensional Planck scale, the experimental effects of these twisted fields may turn out to be larger than the effects of virtual and real KK gravity modes. The collider signatures of anomalous U(1) gauge symmetries as well as other phenomenological implications of the brane world are also discussed.Comment: Published version (paper shortened to satisfy the requirements of Phys. Lett. B; see version 1 for original-length paper

Production of Specific Fragments of {varphi}X174 Replicative Form DNA by a Restriction Enzyme from Haemophilus parainfluenzae, Endonuclease HP

A restriction endonuclease from Haemophilus parainfluenzae degrades {varphi}X174 replicative form DNA into eight specific fragments, ranging from 1,700 to 150 base pairs and terminated specifically by deoxycytidylic acid

Meiotic Silencing by Unpaired DNA

AbstractThe silencing of gene expression by segments of DNA present in excess of the normal number is called cosuppression in plants and quelling in fungi. We describe a related process, meiotic silencing by unpaired DNA (MSUD). DNA unpaired in meiosis causes silencing of all DNA homologous to it, including genes that are themselves paired. A semidominant Neurospora mutant, Sad-1, fails to perform MSUD. Sad-1 suppresses the sexual phenotypes of many ascus-dominant mutants. MSUD may provide insights into the function of genes necessary for meiosis, including genes for which ablation in vegetative life would be lethal. It may also contribute to reproductive isolation of species within the genus Neurospora. The wild-type allele, sad-1+, encodes a putative RNA-directed RNA polymerase

On High-Energy Behavior of Cross Sections in Theories with Large Extra Dimensions

We discuss the high-energy behavior of cross sections in theories with large extra dimensions and low-scale quantum gravity, addressing two particular issues: (i) the tension of the D-branes, and (ii) bounds on the cross section and their relation to approximations in the mode sum over Kaluza-Klein-graviton exchanges.Comment: 6 pages, late

Changing the fate of Fuel Cell Vehicles : can lessons be learnt from Tesla Motors?

AbstractFuel Cell Vehicles (FCVs) are a disruptive innovation and are currently looking towards niche market entry. However, commercialisation has been unsuccessful thus far and there is a limited amount of literature that can guide their market entry. In this paper a historical case study is undertaken which looks at Tesla Motors high-end encroachment market entry strategy. FCVs have been compared to Tesla vehicles due to their similarities; both are disruptive innovations, both are high cost and both are zero emission vehicles. Therefore this paper looks at what can be learned form Tesla Motors successful market entry strategy and proposes a market entry strategy for FCVs. It was found that FCVs need to enact a paradigm shift from their current market entry strategy to one of high-end encroachment. When this has been achieved FCVs will have greater potential for market penetration

Comparing high-end and low-end early adopters of battery electric vehicles

AbstractBattery electric vehicle adoption research has been on going for two decades. The majority of data gathered thus far is taken from studies that sample members of the general population and not actual adopters of the vehicles. This paper presents findings from a study involving 340 adopters of battery electric vehicles. The data is used to corroborate some existing assumptions made about early adopters. The contribution of this paper, however, is the distinction between two groups of adopters. These are high-end adopters and low-end adopters. It is found that each group has a different socio-economic profile and there are also some psychographic differences. Further they have different opinions of their vehicles with high-end adopters viewing their vehicles more preferentially. The future purchase intentions of each group are explored and it is found that high-end adopters are more likely to continue with ownership of battery electric vehicles in subsequent purchases. Finally reasons for this are explored by comparing each adopter group’s opinions of their vehicles to their future purchase intentions. From this is it suggested that time to refuel and range for low-end battery electric vehicles should be improved in order to increase chances of drivers continuing with BEV ownership

Inducing Cross-Clade Neutralizing Antibodies against HIV-1 by Immunofocusing

Background: Although vaccines are important in preventing viral infections by inducing neutralizing antibodies (nAbs), HIV-1 has proven to be a difficult target and escapes humoral immunity through various mechanisms. We sought to test whether HIV-1 Env mimics may serve as immunogens. Methodology/Principal Findings: Using random peptide phage display libraries, we identified the epitopes recognized by polyclonal antibodies of a rhesus monkey that had developed high-titer, broadly reactive nAbs after infection with a simian-human immunodeficiency virus (SHIV) encoding env of a recently transmitted HIV-1 clade C (HIV-C). Phage peptide inserts were analyzed for conformational and linear homology using computational analysis; some peptides mimicked various domains of the original HIV-C Env, such as conformational V3 loop epitopes and the conserved linear region of the gp120 C-terminus. Next, we devised a novel prime/boost strategy to test the immunogenicity of such phage-displayed peptides and primed mice only once with HIV-C gp160 DNA followed by boosting with mixtures of recombinant phages. Conclusions/Significance: This strategy, which was designed to focus the immune system on a few Env epitopes (immunofocusing), not only induced HIV-C gp160 binding antibodies and cross-clade nAbs, but also linked a conserved HIV Env region for the first time to the induction of nAbs: the C-terminus of gp120. The identification of conserved antigen mimics may lead to novel immunogens capable of inducing broadly reactive nAbs

Effects of Gauge Interactions on Fermion Masses in Models with Fermion Wavefunctions Separated in Higher Dimensions

We consider models that generate hierarchies via the separation of fermion wavefunctions in higher-dimensional spaces. We calculate the effects of gauge interactions between fermions and show that these are important and could help to explain (i) why the heaviest known fermion is a charge 2/3 quark, rather than a charge -1/3 quark or a lepton, (ii) why this fermion has a mass $m_t$ comparable to the electroweak symmetry breaking scale $M_{ew}$, (iii) the patterns $m_t >> m_b > m_\tau$ and $m_c >> m_s > m_\mu$, and (iv) the smallness of neutrino masses.Comment: 9 pages, late