An annotated checklist of the Coleoptera (Insecta) of the Cayman Islands, West Indies

A faunal list of 605 species of Coleoptera in 396 genera in 63 families is presented for the Cayman Islands. For most species, island and locality within island collecting information is provided

Barite Deposits of Kentucky

Barite deposits are known to be present in 23 counties in Kentucky, principally in the Central Kentucky Mineral District and the Western Kentucky Fluorspar District. Field investigations and a literature search indicate the presence of barite at more than 170 outcrops, prospects, and abandoned mines. Geologically, most of the Kentucky barite deposits are classified as vein or residual deposits. The vein deposits are cavity and breccia fills along faults and joints, commonly in limestone. Residual deposits occur in an unconsolidated clayey residuum formed by weathering of preexisting vein or breccia deposits. Most deposits are mixed ores commonly containing calcite, fluorite, galena, and sphalerite. Host rocks are almost exclusively formations of Ordovician and Mississippian ages. Barite production in Kentucky has been small, and most has been from residual or gravel\u27\u27 deposits in weathered material above fault zones. Since barite is commonly associated with fluorspar and the two minerals have been difficult to separate in milling operations, mixed ores have been bypassed in a number of instances and little quantitative information is available on them. Further evaluation will require additional exploration, and, particularly, more core drilling. There were no active barite mines in Kentucky at the time of this investigation

Targeted Assembly of Short Sequence Reads

As next-generation sequence (NGS) production continues to increase, analysis is becoming a significant bottleneck. However, in situations where information is required only for specific sequence variants, it is not necessary to assemble or align whole genome data sets in their entirety. Rather, NGS data sets can be mined for the presence of sequence variants of interest by localized assembly, which is a faster, easier, and more accurate approach. We present TASR, a streamlined assembler that interrogates very large NGS data sets for the presence of specific variants, by only considering reads within the sequence space of input target sequences provided by the user. The NGS data set is searched for reads with an exact match to all possible short words within the target sequence, and these reads are then assembled strin-gently to generate a consensus of the target and flanking sequence. Typically, variants of a particular locus are provided as different target sequences, and the presence of the variant in the data set being interrogated is revealed by a successful assembly outcome. However, TASR can also be used to find unknown sequences that flank a given target. We demonstrate that TASR has utility in finding or confirming ge-nomic mutations, polymorphism, fusion and integration events. Targeted assembly is a powerful method for interrogating large data sets for the presence of sequence variants of interest. TASR is a fast, flexible and easy to use tool for targeted assembly

Engineering truly automated data integration and translation systems

This thesis presents an automated, data-driven integration process for relational databases. Whereas previous integration methods assumed a large amount of user involvement as well as the availability of database meta-data, we make no use of meta-data and little end user input. This is done using a novel join and translation finding algorithm that searches for the proper key / foreign key relationships while inferring the instance transformations from one database to another. Because we rely only on the relations that bind the attributes together, we make no use of the database schema information. A novel searching method allows us to search the database for relevant objects without requiring server side indexes or cooperative databases

Interactions between subunits a and b in the rotary ATP synthase as determined by cross-linking

The interaction of the membrane traversing stator subunits a and b of the rotary ATP synthase was probed by substitution of a single Cys into each subunit with subsequent Cu2+ catalyzed cross-linking. Extensive interaction between the transmembrane (TM) region of one b subunit and TM2 of subunit a was indicated by cross-linking with 6 Cys pairs introduced into these regions. Additional disulfide cross-linking was observed between the N-terminus of subunit b and the periplasmic loop connecting TM4 and TM5 of subunit a. Finally, benzophenone-4-maleimide derivatized Cys in the 2–3 periplasmic loop of subunit a were shown to cross-link with the periplasmic N-terminal region of subunit b. These experiments help to define the juxtaposition of subunits b and a in the ATP synthase

Chemical spray pyrolysis of Tl-Ba-Ca-Cu-O high-T(sub c) superconductors for high-field bitter magnets

The deposition of Tl-Ba-Ca-Cu-O thick films by spray pyrolyzing a Ba-Ca-Cu-O precursor film and diffusing thallium into the film to form the superconducting phase is examined. This approach was taken to reduce exposure to thallium and its health and safety hazards. The Tl-Ba-Ca-Cu-O system was selected because it has very attractive features which make it appealing to device and manufacturing engineering. Tl-Ba-Ca-Cu-O will accommodate a number of superconducting phases. This attribute makes it very forgiving to stoichiometric fluctuations in the bulk and film. It has excellent thermal and chemical stability, and appears to be relatively insensitive to chemical impurities. Oxygen is tightly bound into the systems, consequently there is no orthorhombic (conductor) to tetragonal (insulator) transition which would affect a component's lifetime. More significantly, the thallium based superconductors appear to have harder magnetic properties than the other high-Tc oxide ceramics. Estimates using magnetoresistance measurements indicate that at 77 K Tl2Ba2CaCu2O10 will have an upper critical field, H(sub c2) fo 26 Tesla for applied fields parallel to the c-axis and approximately 1000 Tesla for fields oriented in the a-b plane. Results to date have shown that superconducting films can be reproducibly deposited on 100 oriented MgO substrates. One film had a zero resistance temperature of 111.5 K. Furthermore, x ray diffraction analysis of the films showed preferential c-axis orientation parallel to the plane of the substrate. These results have now made it possible to consider the manufacture of a superconducting tape wire which can be configured into a topology useful for high-field magnet designs. The research which leads to the preparation of these films and plans for further development are reviewed

"Making Safety Happen" Through Probabilistic Risk Assessment at NASA

NASA is using Probabilistic Risk Assessment (PRA) as one of the tools in its Safety & Mission Assurance (S&MA) tool belt to identify and quantify risks associated with human spaceflight. This paper discusses some of the challenges and benefits associated with developing and using PRA for NASA human space programs. Some programs have entered operation prior to developing a PRA, while some have implemented PRA from the start of the program. It has been observed that the earlier a design change is made in the concept or design phase, the less impact it has on cost and schedule. Not finding risks until the operation phase yields much costlier design changes and major delays, which can result in discussions of just accepting the risk. Risk contributors identified by PRA are not just associated with hardware failures. They include but are not limited to crew fatality due to medical causes, the environment the vehicle and crew are exposed to, the software being used, and the reliability of the crew performing required actions. Some programs have entered operation prior to developing a PRA, and while PRA can still provide a benefit for operations and future design trades, the benefit of implementing PRA from the start of the program provides the added benefit of informing design and reducing risk early in program development. Currently, NASAs International Space Station (ISS) program is in its 20th year of on-orbit operations around the Earth and has several new programs in the design phase preparing to enter the operation phase all of which have active (or living) PRAs. These programs incorporate PRA as part of their Risk-Informed, Decision-Making (RIDM) process. For new NASA human spaceflight programs discussion begins with mission concept, establishing requirements, forming the PRA team, and continues through the design cycles into the operational phase. Several examples of PRA related applications and observed lessons are included

Preliminary analysis of LDEF instrument A0187-1: Chemistry of Micrometeoroids Experiment

The Chemistry of Micrometeoroids Experiment (CME) exposed approximately 0.8 sq. m of gold on the Long Duration Exposure Facility's (LDEF's) trailing edge (location A03) and approximately 1.1 sq. m of aluminum in the forward-facing A11 location. The most significant results to date relate to the discovery of unmelted pyroxene and olivine fragments associated with natural cosmic dust impacts. The latter are sufficiently large for detailed phase studies, and they serve to demonstrate that recovery of unmelted dust fragments is a realistic prospect for further dust experiments that will employ more advanced collector media. We also discovered that man-made debris impacts occur on the LDEF's trailing edge with substantially higher frequency than expected, suggesting that orbital debris in highly elliptical orbits may have been somewhat underestimated