Mass Transfer and Accretion in the Eccentric Neutron-Star Binary Circinus X-1

I have carried out a project to study the eccentric neutron-star binary Circinus X-1 through an extensive series of observational studies with the Rossi X-ray Timing Explorer satellite and through theoretical computer models I developed to explore mass transfer and evolution in an eccentric binary. I modeled the evolution of the energy spectrum during intensity dips with a variably absorbed bright component plus a fainter unabsorbed component. I show that variability not attributable to absorption dips is related to the spectral/intensity states of the ``Z source'' class of low-mass X-ray binaries (LMXBs), namely motion along (or shifts of) the horizontal, normal, and flaring branches of the ``Z'' track in color-color and hardness-intensity diagrams. I found rapid X-ray variability properties associated with each spectral/intensity state: On the horizontal branch, quasi-periodic oscillations (QPOs) in the X-ray intensity shift in frequency from 1.3 to 35 Hz. On the normal branch, a different QPO occurs at about 4 Hz. On the flaring branch only strong aperiodic variability occurs. I also modeled the evolution of the energy spectra associated with each of these branches. To study mass transfer in an eccentric binary, I developed computer codes for transfer via Roche-lobe overflow and from a stellar wind. I derive theoretical mass accretion profiles and compare them to the observed profile of the X-ray intensity. In order to explore the possible evolutionary history of Circinus X-1, I developed a binary-evolution computer code for a neutron-star and low-mass companion in an eccentric orbit. I use this code in a population-synthesis study to show that the number of systems in the Galaxy expected to resemble Cir X-1 is of order unity, consistent with its unique status as an LMXB with high eccentricity.Comment: Ph.D. Thesis (M.I.T.): 206 pages, 103 postscript figures. Also available at http://space.mit.edu/~shirey/thesis.htm

First XMM-Newton observations of strongly magnetic cataclysmic variables - II. Timing studies of DP Leo and WW Hor

XMM-Newton was used to observe two eclipsing, magnetic cataclysmic variables, DP Leo and WW Hor, continuously for three orbital cycles each. Both systems were in an intermediate state of accretion. For WW Hor we also obtained optical light curves with the XMM-Newton Optical Monitor and from ground-based observations. Our analysis of the X-ray and optical light curves allows us to constrain physical and geometrical parameters of the accretion regions and derive orbital parameters and eclipse ephemerides of the systems. For WW Hor we directly measure horizontal and vertical temperature variations in the accretion column. From comparisons with previous observations we find that changes in the accretion spot longitude are correlated with the accretion rate. For DP Leo the shape of the hard X-ray light curve is not as expected for optically thin emission, showing the importance of optical depth effects in the post-shock region. We find that the spin period of the white dwarf is slightly shorter than the orbital period and that the orbital period is decreasing faster than expected for energy loss by gravitational radiation alone.Comment: Accepted for publication in MNRAS, 12 pages, 6 figure

Scattering and Iron Fluorescence Revealed During Absorption Dips in Circinus X-1

We show that dramatic spectral evolution associated with dips occurring near phase zero in RXTE observations of Cir X-1 is well-fit by variable and at times heavy absorption (N_H > 10^24 cm^-2) of a bright component, plus an underlying faint component which is not attenuated by the variable column and whose flux is ~10% of that of the unabsorbed bright component. A prominent Fe emission line at ~6.5 keV is evident during the dips. The absolute line flux outside the dips is similar to that during the dips, indicating that the line is associated with the faint component. These results are consistent with a model in which the bright component is radiation received directly from a compact source while the faint component may be attributed to scattered radiation. Our results are also generally consistent with those of Brandt et al., who found that a partial- covering model could explain ASCA spectra of a low-to-high transition in Cir X-1. The relative brightness of the two components in our model requires a column density of ~2*10^23 cm^-2 if the faint component is due to Thomson scattering in material that mostly surrounds the source. We find that illumination of such a scattering cloud by the observed direct component would produce an Fe K-alpha fluorescence flux that is in rough agreement with the flux of the observed emission line. We also conclude that if the scattering medium is not highly ionized, our line of sight to the compact source does not pass through it. Finally, we discuss simple pictures of the absorbers responsible for the dips themselves.Comment: Accepted for publication in The Astrophysical Journal (23 pages, including 11 figures

Pancreatic ductal adenocarcinoma cell secreted extracellular vesicles containing ceramide-1-phosphate promote pancreatic cancer stem cell motility

The high mortality rate associated with pancreatic ductal adenocarcinoma (PDAC) is in part due to lack of effective therapy for this highly chemoresistant tumor. Cancer stem cells, a subset of cancer cells responsible for tumor initiation and metastasis, are not targeted by conventional cytotoxic agents, which renders the identification of factors that facilitate cancer stem cell activation useful in defining targetable mechanisms. We determined that bioactive sphingolipid induced migration of pancreatic cancer stem cells (PCSC) and signaling was specific to ceramide-1-phosphate (C1P). Furthermore, PDAC cells were identified as a rich source of C1P. Importantly, PDAC cells express the C1P converting enzyme ceramide kinase (CerK), secrete C1P-containing extracellular vesicles that mediate PCSC migration, and when co-injected with PCSC reduce animal survival in a PDAC peritoneal dissemination model. Our findings suggest that PDAC secrete C1P-containing extracellular vesicles as a means of recruiting PCSC to sustain tumor growth therefore making C1P release a mechanism that could facilitate tumor progression

Autocrine Regulation of Pulmonary Inflammation by Effector T-Cell Derived IL-10 during Infection with Respiratory Syncytial Virus

Respiratory syncytial virus (RSV) infection is the leading viral cause of severe lower respiratory tract illness in young infants. Clinical studies have documented that certain polymorphisms in the gene encoding the regulatory cytokine IL-10 are associated with the development of severe bronchiolitis in RSV infected infants. Here, we examined the role of IL-10 in a murine model of primary RSV infection and found that high levels of IL-10 are produced in the respiratory tract by anti-viral effector T cells at the onset of the adaptive immune response. We demonstrated that the function of the effector T cell -derived IL-10 in vivo is to limit the excess pulmonary inflammation and thereby to maintain critical lung function. We further identify a novel mechanism by which effector T cell-derived IL-10 controls excess inflammation by feedback inhibition through engagement of the IL-10 receptor on the antiviral effector T cells. Our findings suggest a potentially critical role of effector T cell-derived IL-10 in controlling disease severity in clinical RSV infection

The human body at cellular resolution: the NIH Human Biomolecular Atlas Program

Abstract: Transformative technologies are enabling the construction of three-dimensional maps of tissues with unprecedented spatial and molecular resolution. Over the next seven years, the NIH Common Fund Human Biomolecular Atlas Program (HuBMAP) intends to develop a widely accessible framework for comprehensively mapping the human body at single-cell resolution by supporting technology development, data acquisition, and detailed spatial mapping. HuBMAP will integrate its efforts with other funding agencies, programs, consortia, and the biomedical research community at large towards the shared vision of a comprehensive, accessible three-dimensional molecular and cellular atlas of the human body, in health and under various disease conditions

A global phylogeny of butterflies reveals their evolutionary history, ancestral hosts and biogeographic origins

Butterflies are a diverse and charismatic insect group that are thought to have evolved with plants and dispersed throughout the world in response to key geological events. However, these hypotheses have not been extensively tested because a comprehensive phylogenetic framework and datasets for butterfly larval hosts and global distributions are lacking. We sequenced 391 genes from nearly 2,300 butterfly species, sampled from 90 countries and 28 specimen collections, to reconstruct a new phylogenomic tree of butterflies representing 92% of all genera. Our phylogeny has strong support for nearly all nodes and demonstrates that at least 36 butterfly tribes require reclassification. Divergence time analyses imply an origin similar to 100 million years ago for butterflies and indicate that all but one family were present before the K/Pg extinction event. We aggregated larval host datasets and global distribution records and found that butterflies are likely to have first fed on Fabaceae and originated in what is now the Americas. Soon after the Cretaceous Thermal Maximum, butterflies crossed Beringia and diversified in the Palaeotropics. Our results also reveal that most butterfly species are specialists that feed on only one larval host plant family. However, generalist butterflies that consume two or more plant families usually feed on closely related plants