Screening Pinus sylvestris Grown for the Production of Christmas Trees for Resistance to Western Gall Rust Peridermium harknessii Using Different Sources of Aeciospores

Results showed a moderate to high susceptibility of Pinus sylvestris to western gall rust Peridermium harknessii from Pinus sylvestris in Michigan and Pinus banksiana in Minnesota. In general, Pinus sylvestris seed sources were more susceptible to aeciospores collected from Pinus sylvestris than aeciospores collected from Pinus banksiana

Environmental pollutants from the Scott and Shackleton expeditions during the ‘Heroic Age’ of Antarctic exploration

Early explorers to Antarctica built wooden huts and brought huge quantities of supplies and equipment to support their geographical and scientific studies for several years. When the expeditions ended and relief ships arrived, a rapid exodus frequently allowed only essential items to be taken north. The huts and thousands of items were left behind. Fuel depots with unused containers of petroleum products, asbestos materials, and diverse chemicals were also left at the huts. This investigation found high concentrations of polyaromatic hydrocarbons in soils under and around the historic fuel depots, including anthracene, benzo[b]fluoranthene, benzo[k]fluoranthene, chrysene, fluorene, and pyrene, as well as benzo[a]anthracene, benzo[a]pyrene, and fluoranthene, which are recognized carcinogens. Asbestos materials within the huts have been identified and extensive amounts of fragmented asbestos were found littering the ground around the Cape Evans hut. These materials are continually abraded and fragmented as tourists walk over them and the coarse scoria breaks and grinds down the materials. A chemical spill, within the Cape Evans hut, apparently from caustic substances from one of the scientific experiments, has caused an unusual deterioration and defibration on affected woods. Although these areas are important historic sites protected by international treaties, the hazardous waste materials left by the early explorers should be removed and remedial action taken to restore the site to as pristine a condition as possible. Recommendations are discussed for international efforts to study and clean up these areas, where the earliest environmental pollution in Antarctica was produced

Effect of Wood Particle Size on Fungal Growth in a Model Biomechanical Pulping Process

The pretreatment of aspen wood chips with white-rot fungus has been evaluated as a way of making biomechanical pulp. Our study addressed (1) whether wood particle size (chip size) affects the growth pattern of the attacking organism, and (2) whether the difference in particle size between chips and coarse pulp is related to the availability of wood polymers to the fungus. We qualitatively evaluated the growth of Phanerochaete chrysosporium BKM-F-1767 on aspen wood using standard industrial 6- and 19-mm chips and coarse refiner mechanical pulp. Scanning electron microscopy revealed a slight increase in the number of hyphae in the 19-mm chips compared to that in the 6-mm chips, but no major morphological differences in cellulose or lignin loss. Dense aerial hyphal growth occurred around the chips, but not around the coarse pulp. The fungus appeared to attack the coarse pulp from both outside and within the fiber wall. Hyphae within both the middle lamella and the cell lumina attacked the cell walls. The fungus eroded the chip cell walls and their constituents primarily from the wood cell lumen outward. After only 3 weeks of fungal treatment, both chips and coarse pulp showed marked localized cell-wall thinning and fragmentation as well as generalized swelling and relaxing of the normally rigid cell-wall structure. We conclude that particle size has only a minor effect on fungal growth on wood under conditions such as those likely to be used in a commercial biopulping process

Screening fungi isolated from historic Discovery Hut on Ross Island, Antarctica for cellulose degradation

To survive in Antarctica, early explorers of Antarctica's Heroic Age erected wooden buildings and brought in large quantities of supplies. The introduction of wood and other organic materials may have provided new nutrient sources for fungi that were indigenous to Antarctica or were brought in with the materials. From 30 samples taken from Discovery Hut, 156 filamentous fungi were isolated on selective media. Of these, 108 were screened for hydrolytic activity on carboxymethyl cellulose, of which 29 demonstrated activities. Endo-1, 4-β-glucanase activity was confirmed in the extracellular supernatant from seven isolates when grown at 4°C, and also when they were grown at 15°C. Cladosporium oxysporum and Geomyces sp. were shown to grow on a variety of synthetic cellulose substrates and to use cellulose as a nutrient source at temperate and cold temperatures. The research findings from the present study demonstrate that Antarctic filamentous fungi isolated from a variety of substrates (wood, straw, and food stuffs) are capable of cellulose degradation and can grow well at low temperatures

Evolution of substrate-specific gene expression and RNA editing in brown rot wood-decaying fungi.

Fungi that decay wood have characteristic associations with certain tree species, but the mechanistic bases for these associations are poorly understood. We studied substrate-specific gene expression and RNA editing in six species of wood-decaying fungi from the 'Antrodia clade' (Polyporales, Agaricomycetes) on three different wood substrates (pine, spruce, and aspen) in submerged cultures. We identified dozens to hundreds of substrate-biased genes (i.e., genes that are significantly upregulated in one substrate relative to the other two substrates) in each species, and these biased genes are correlated with their host ranges. Evolution of substrate-biased genes is associated with gene family expansion, gain and loss of genes, and variation in cis- and trans- regulatory elements, rather than changes in protein coding sequences. We also demonstrated widespread RNA editing events in the Antrodia clade, which differ from those observed in the Ascomycota in their distribution, substitution types, and the genomic environment. Moreover, we found that substrates could affect editing positions and frequency, including editing events occurring in mRNA transcribed from wood-decay-related genes. This work shows the extent to which gene expression and RNA editing differ among species and substrates, and provides clues into mechanisms by which wood-decaying fungi may adapt to different hosts

Transcriptomics of Temporal- versus Substrate-Specific Wood Decay in the Brown-Rot Fungus Fibroporia radiculosa

Brown-rot fungi lack many enzymes associated with complete wood degradation, such as lignin-attacking peroxidases, and have developed alternative mechanisms for rapid wood breakdown. To identify the effects of culture conditions and wood substrates on gene expression, we grew Fibroporia radiculosa in submerged cultures containing Wiley milled wood (5 days) and solid wood wafers (30 days), using aspen, pine, and spruce as a substrate

Species of Mycosphaerellaceae and Teratosphaeriaceae on native Myrtaceae in Uruguay : evidence of fungal host jumps

Mycosphaerella species are well-known causal agents of leaf diseases on many economically and ecologically important plant species. In Uruguay, a relatively large number of Mycosphaerellaceae and Teratosphaeriaceae are found on Eucalyptus, but nothing is known of these fungi on native Myrtaceae. The aim of this study was to identify Mycosphaerellaceae and Teratosphaeriaceae species associated with leaf diseases on native Myrtaceae in Uruguay and to consider whether host jumps by the pathogen from introduced Eucalyptus to native Myrtaceae have occurred. Several native forests throughout the country were surveyed with special attention given to those located close to Eucalyptus plantations. Five species belonging to the Mycosphaerellaceae and Teratosphaeriaceae clades were found on native Myrtaceous trees and three of these had previously been reported on Eucalyptus in Uruguay. Those occurring both on Eucalyptus and native Myrtaceae included Pallidocercospora heimii, Pseudocercospora norchiensis, and Teratosphaeria aurantia. In addition, Mycosphaerella yunnanensis, a species known to occur on Eucalyptus but not previously recorded in Uruguay, was found on leaves of two native Myrtaceous hosts. Because most of these species occur on Eucalyptus in countries other than Uruguay, it appears that they were introduced in this country and have adapted to be able to infect native Myrtaceae. These apparent host jumps have the potential to result in serious disease problems and they should be carefully monitored.Forestal Oriental, Rivermol, Stora Enso and Weyerhaeuser.http://www.elsevier.com/locate/funbiohb201

Formalizing Bachmair and Ganzinger’s Ordered Resolution Prover

We present a formalization of the first half of Bachmair and Ganzinger’s chapter on resolution theorem proving in Isabelle/HOL, culminating with a refutationally complete first-order prover based on ordered resolution with literal selection. We develop general infrastructure and methodology that can form the basis of completeness proofs for related calculi, including superposition. Our work clarifies several of the fine points in the chapter’s text, emphasizing the value of formal proofs in the field of automated reasoning

Three new genera of fungi from extremely acidic soils

Extremely acidic soils (pH<3) harbour poorly diversified mycobiota that are very different from less acidic habitats. During investigations of the mycobiota from several highly acidic soils in the Czech Republic and a coastal site in the Antarctic Peninsula, a group of hyaline fungal isolates was obtained. Based on phenotype and nuclear ribosomal DNA sequences (ITS region, SSU, LSU), the isolates belonged to three phylogenetic lineages within two different classes, Sordariomycetes and Leotiomycetes (Pezizomycotina, Ascomycota). The first lineage is described here as a new genus and species Acidothrix acidophila gen. nov. et sp. nov. (Amplistromataceae, Sordariomycetes, Ascomycota). The most closely related species to this new clade are woodinhabiting fungi. The isolates belonging to the second and the third lineages are also described as two new genera and species Acidea extrema gen. nov. et sp. nov. and Soosiella minima gen. nov. et sp. nov. (Helotiales, Leotiomycetes, Ascomycota). Their position and the relationships within Helotiales are discussed. Soosiella minima was acidotolerant, Acidothrix acidophila and Acidea extrema exhibited both acidotolerant and acidophilic characteristics. All the species were slightly halophilic. The adaptation of hyaline fungi from mesophilic lineages to highly acidic environments has been revealed. The association between highly acidic and Antarctic habitats is discussed.http://link.springer.com/journal/11557hb201

Identifying the “Mushroom of Immortality”: Assessing the Ganoderma Species Composition in Commercial Reishi Products

Species of Ganoderma, commonly called reishi (in Japan) or lingzhi (in China), have been used in traditional medicine for thousands of years, and their use has gained interest from pharmaceutical industries in recent years. Globally, the taxonomy of Ganoderma species is chaotic, and the taxon name Ganoderma lucidum has been used for most laccate (shiny) Ganoderma species. However, it is now known that G. lucidum sensu stricto has a limited native distribution in Europe and some parts of China. It is likely that differences in the quality and quantity of medicinally relevant chemicals occur among Ganoderma species. To determine what species are being sold in commercially available products, twenty manufactured products (e.g., pills, tablets, teas, etc.) and seventeen grow your own (GYO) kits labeled as containing G. lucidum were analyzed. DNA was extracted, and the internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (tef1α) were sequenced with specific fungal primers. The majority (93%) of the manufactured reishi products and almost half of the GYO kits were identified as Ganoderma lingzhi. G. lingzhi is native to Asia and is the most widely cultivated and studied taxon for medicinal use. Illumina MiSeq sequencing of the ITS1 region was performed to determine if multiple Ganoderma species were present. None of the manufactured products tested contained G. lucidum sensu stricto, and it was detected in only one GYO kit. G. lingzhi was detected in most products, but other Ganoderma species were also present, including G. applanatum, G. australe, G. gibbosum, G. sessile, and G. sinense. Our results indicate that the content of these products vary and that better labeling is needed to inform consumers before these products are ingested or marketed as medicine. Of the 17 GYO kits tested, 11 kits contained Ganoderma taxa that are not native to the United States. If fruiting bodies of exotic Ganoderma taxa are cultivated, these GYO kits will likely end up in the environment. The effects of these exotic species to natural ecosystems needs investigation