Antigen-presenting cells and antigen presentation in tertiary lymphoid organs

Tertiary lymphoid organs (TLOs) form in territorialized niches of peripheral tissues characterized by the presence of antigens; however, little is known about mechanism(s) of antigen handling by ectopic lymphoid structures. In this mini review, we will discuss the role of antigen-presenting cells and mechanisms of antigen presentation in TLOs, summarizing what is currently known about this facet of the formation and function of these tissues as well as identifying questions yet to be addressed

THE AGRICULTURAL RISK MANAGEMENT SIMULATOR MICROCOMPUTER PROGRAM

The Agricultural Risk Management Simulator (ARMS) is a microcomputer program designed to help users evaluate strategies for managing yield and price risk in crop farming operations. Risk management strategies are defined by choices regarding crop mix, the purchase of multiple peril crop insurance, and the use of forward contracting. Probabilistic budgeting is used to determine the net cash flow probability distribution for each strategy considered. Flexibility with regard to both sources of probabilistic information and the form of yield and price probability distributions is a noteworthy feature of the program.Risk and Uncertainty,

Wrack Lines Volume 21, Number 1, Born Out of Crises: responses, research and reflections on a better future

Articles in this issue explore various actions taken in response to different crises: lessons about the environment from the COVID-19 pandemic; how seafood sellers in the CT and Southeast Asia responded to the challenges of the pandemic; how the challenges of rising seas and developed coasts are being dealt with through managed retreat, buyouts and other actions in NC and CT; and research on the long-term effects of the Deepwater Horizon oil spill on dolphins led by CTSG Director Sylvain De Guise

Mapping lung cancer epithelial-mesenchymal transition states and trajectories with single-cell resolution.

Elucidating the spectrum of epithelial-mesenchymal transition (EMT) and mesenchymal-epithelial transition (MET) states in clinical samples promises insights on cancer progression and drug resistance. Using mass cytometry time-course analysis, we resolve lung cancer EMT states through TGFβ-treatment and identify, through TGFβ-withdrawal, a distinct MET state. We demonstrate significant differences between EMT and MET trajectories using a computational tool (TRACER) for reconstructing trajectories between cell states. In addition, we construct a lung cancer reference map of EMT and MET states referred to as the EMT-MET PHENOtypic STAte MaP (PHENOSTAMP). Using a neural net algorithm, we project clinical samples onto the EMT-MET PHENOSTAMP to characterize their phenotypic profile with single-cell resolution in terms of our in vitro EMT-MET analysis. In summary, we provide a framework to phenotypically characterize clinical samples in the context of in vitro EMT-MET findings which could help assess clinical relevance of EMT in cancer in future studies

The risk of pathogenic intestinal parasite infections in Kisii Municipality, Kenya

<p>Abstract</p> <p>Background</p> <p>Intestinal parasitic infections are among the most common infections worldwide. Various epidemiological studies indicate that the prevalence of intestinal parasites is high especially in developing countries, although in many of these, the environmental risk factors have not been clearly elucidated. The objective of this study was to determine the risk of pathogenic intestinal parasites infections in Kisii Municipality.</p> <p>Methods</p> <p>Random sampling was used in the selection of the study samples. Stool parasitological profiles of food handlers were done by direct smear and formalin-ethyl acetate sedimentation method. Both vegetable and meat samples were examined for the presence of intestinal parasites. The storage and meat handling practices of the various butcheries were observed.</p> <p>Results</p> <p>Types of samples examined for occurrence of intestinal parasites includes, a total of 84 vegetable, 440 meat and 168 stool samples. Fifty five (65.5%) vegetable, 334 (75.9%) meat and 69 (41.1%) of the stool samples were found positive for intestinal parasites indicating a high overall risk (66.18%) for intestinal parasite infections. Of the parasites detected, the most common parasites infesting the foodstuffs and infecting the food handlers were <it>Ascaris lumbricoides </it>and <it>Entamoeba histolytica</it>. Parasites were significantly less likely to be present on meat that was refrigerated during display than meat that was displayed at ambient temperature.</p> <p>Conclusion</p> <p>There is a high risk of infection with intestinal parasites in the sampled Municipal markets. About half of the food handlers surveyed (41.1 %) at the Municipal Hospital had one or more parasitic infections. Furthermore, meat (65.5%) and vegetables (75.9%) sold at the Municipal market were found to be contaminated with parasites hence the inhabitants requires a need for education on food safety, good distribution practices and improvement on sanitary conditions.</p

Role of σ\u3csup\u3eB\u3c/sup\u3e in Adaptation of \u3ci\u3eListeria monocytogenes\u3c/i\u3e to Growth at Low Temperature

The activity of σB in Listeria monocytogenes is stimulated by high osmolarity and is necessary for efficient uptake of osmoprotectants. Here we demonstrate that, during cold shock, σB contributes to adaptation in a growth phase-dependent manner and is necessary for efficient accumulation of betaine and carnitine as cryoprotectants

Supracervical Robotic-Assisted Laparoscopic Sacrocolpopexy for Pelvic Organ Prolapse

Supracervical robotic-assisted laparoscopic sacrocolpopexy was found to be an effective repair of apical vaginal defects in patients with pelvic organ prolapse who had not undergone previous hysterectomy

Altitude Variation of the Plasmapause Signature in the Main Ionospheric Trough

The projection of the plasmapause magnetic-field lines to low altitudes, where the light-ion chemistry is dominated by O(+), tends to occur near the minimum electron density in the main (midlatitude) electron density trough at night. With increasing attitude in the trough, where H(+) emerges as the dominant iota on the low-latitude boundary, we have found cases where the plasmapause field lines are located on the sharp low-Latitude side of the trough as expected if this topside ionosphere H(+) distribution varies in step with the plasmapause gradient in the distant plasmasphere. These conclusions are based on near-equatorial crossings of the plasmapause (corresponding to the steep gradient in the dominant species H(+) by the Explorer-45 satellite as determined from electric-field measurements by Maynard and Cauffman in the early 1970s and ISIS-2 ionospheric topside-sounder measurements. The former data have now been converted to digital form and made available at http://nssdcftp.gsfc.nasa.gov. The latter provide samples of nearly coincident observations of ionospheric main trough crossings near the same magnetic-field lines of the Explorer 45-determined equatorial plasmapause. The ISIS-2 vertical electron density profiles are used to infer where the F-region transitions from an O(+) to a H(+) dominated plasma through the main trough boundaries

Role of arginase 2 in systemic metabolic activity and adipose tissue fatty acid metabolism in diet-induced obese mice

Visceral adipose tissue (VAT) inflammation and metabolic dysregulation are key components of obesity-induced metabolic disease. Upregulated arginase, a ureahydrolase enzyme with two isoforms (A1-cytosolic and A2-mitochondrial), is implicated in pathologies associated with obesity and diabetes. This study examined A2 involvement in obesity-associated metabolic and vascular disorders. WT and globally deleted A2(&#8722;/&#8722;) or A1(+/&#8722;) mice were fed either a high fat/high sucrose (HFHS) diet or normal diet (ND) for 16 weeks. Increases in body and VAT weight of HFHS-fed WT mice were abrogated in A2&#8722;/&#8722;, but not A1+/&#8722;, mice. Additionally, A2&#8722;/&#8722; HFHS-fed mice exhibited higher energy expenditure, lower blood glucose, and insulin levels compared to WT HFHS mice. VAT and adipocytes from WT HFHS fed mice showed greater A2 expression and adipocyte size and reduced expression of PGC-1&#945;, PPAR-&#947;, and adiponectin. A2 deletion blunted these effects, increased levels of active AMPK-&#945;, and upregulated genes involved in fatty acid metabolism. A2 deletion prevented HFHS-induced VAT collagen deposition and inflammation, which are involved in adipocyte metabolic dysfunction. Endothelium-dependent vasorelaxation, impaired by HFHS diet, was significantly preserved in A2&#8722;/&#8722; mice, but more prominently maintained in A1+/&#8722; mice. In summary, A2 is critically involved in HFHS-induced VAT inflammation and metabolic dysfunction