Dietary intake in kickboxing fighters/ Consumo alimentar de lutadores de kickboxing

Kickboxing is a combat sport modality which prioritizes classifying competitors by weight categories for their participation. The aim of this study was to analyze Kickboxing athletes’food intake. Fourteen Kickboxing fighters aged seventeen to twenty-eight participated in the study: twelve men and two women. The 24-hour food recall was used as an instrument. Athletes' food intake data was analyzed in the Virtual Nutri Plus® program. Average daily caloric intake was below the limit recommended in both sexes (2142.85 kcal/day –2125.74 kcal/day median). As for macronutrients; carbohydrates (484g ± 302g) 70% of the sample, lipids (71.3g ± 28.4) 60% and protein (1.76 ± 1.03 g/kg weight) 22% were all below the recommended amount. Fiber consumption  was 60% lower  than recommended, while 50% of volunteers consumed sodium above the amount recommended. This study found inadequacies in the consumption of macronutrients and micronutrients, underlining the importance of multidisciplinary  work  between nutritionists and other professionals  responsible for an athlete’s performance

Has decentralisation affected child immunisation status in Indonesia?

Background: The past two decades have seen many countries, including a number in Southeast Asia, decentralising their health system with the expectation that this reform will improve their citizens’ health. However, the consequences of this reform remain largely unknown. Objective: This study analyses the effects of fiscal decentralisation on child immunisation status in Indonesia. Design: We used multilevel logistic regression analysis to estimate these effects, and multilevel multiple imputation to manage missing data. The 2011 publication of Indonesia's national socio-economic survey (Susenas) is the source of household data, while the Podes village census survey from the same year provides village-level data. We supplement these with local government fiscal data from the Ministry of Finance. Results: The findings show that decentralising the fiscal allocation of responsibilities to local governments has a lack of association with child immunisation status and the results are robust. The results also suggest that increasing the number of village health centres (posyandu) per 1,000 population improves probability of children to receive full immunisation significantly, while increasing that of hospitals and health centres (puskesmas) has no significant effect. Conclusion: These findings suggest that merely decentralising the health system does not guarantee improvement in a country's immunisation coverage. Any successful decentralisation demands good capacity and capability of local governments

Molecular cloning and recombinant expression of the VP28 carboxyl-terminal hydrophilic region from a brazilian white spot syndrome virus isolate

In the present study, a fragment of the VP28 coding sequence from a Brazilian WSSV isolate (BrVP28) was cloned, sequenced and expressed in E. coli BL21(DE3) pLysS strain in order to produce the VP28 carboxyl-terminal hydrophilic region. The expression resulted in a protein of about 21 kDa, which was purified under denaturing conditions, resulting in a final highly purified BrVP28 preparation. The recombinant protein obtained can be used in several biotechnology applications, such as the production of monoclonal antibodies which could be used in the development of diagnostic tools as well as in the studies on the characterization of white spot syndrome virus (WSSV) isolated in Brazil