Effects of fluoxetine on fish - Dataset - Margiotta-Casaluci et al. 2014

The file provides the results of a study during which male fathead minnows were exposed for 28 days to the anti-depressant fluoxetine

Effect of fluoxetine on fish exploratory behaviour quantified during a Novel Tank Diving Test performed after 14 days of exposure.

<p>A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area. C1 and C2 indicate control group 1 and control group 2, respectively. Boxes represent medians (full line), with 5th and 95th percentiles (<i>n</i> = 20). *<i>p</i><0.05.</p

Methodological procedure for the quantification of fish behaviour.

<p>A) Experimental steps performed to quantify fish behavioural response to a new environment following 14-day and 28-day exposure to fluoxetine. Anxiety-related behavioural endpoints were quantified using a Novel Tank Diving Test. B) Example of different exploratory behaviours in a Novel Tank Diving Test. Inactive fish (left) <i>versus</i> active fish (right). The different tracking colours indicate different speeds (black, slow; green, medium; red, fast).</p

Relationship between plasma concentrations of norfluoxetine and its effects on fish exploratory behaviour after 28 days of exposure.

<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine plotted in the graphs is 72–258 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as mean ± SD (<i>n</i> = 20). *: <i>p</i><0.05.</p

Inter-individual and intra-treatment variability of fluoxetine plasma concentrations.

<p>The unit of the values indicated in the columns Mean, Median, Min, Max, 25%, and 75% is ng/mL.</p><p>Inter-individual and intra-treatment variability of fluoxetine plasma concentrations.</p

Relationship between plasma concentrations of fluoxetine plus norfluoxetine and their effects on fish exploratory behaviour after 28 days of exposure.

<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine + norfluoxetine plotted in the graphs is 163–560 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as mean ± SD (<i>n</i> = 20). *: <i>p</i><0.05.</p

Relationship between plasma concentrations of fluoxetine and behavioural endpoints expressed as median values.

<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test after 28 days of exposure. The dose-response of behavioural endpoints appears to be visually more obvious when median values are used (in this figure) instead of mean values (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0110467#pone-0110467-g004" target="_blank">Figure 4</a>). This highlights the important role of inter-individual variability in the interpretation of behavioural effects. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine plotted in the graphs is 91–302 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as medians (<i>n</i> = 20). *: <i>p</i><0.05.</p

Relationship between plasma concentrations of fluoxetine and its effects on fish exploratory behaviour after 28 days of exposure.

<p>Exploratory behaviour was quantified in individual fish using the Novel Tank Diving Test. A) Number of transitions into the Top Area; B) number of transitions into the Middle Area; C) time spent in the Top Area; D) time spent in the Middle Area; E) distance travelled in the Top Area; F) distance travelled in the Middle Area; G) speed. The Human Therapeutic Plasma Concentration range of fluoxetine plotted in the graphs is 91–302 ng/mL. C1 and C2 indicate control group 1 and control group 2, respectively. The X-axis has a Log2 scale, while the Y-axis has a linear scale. Values are plotted as mean ± SD (<i>n</i> = 20). *: <i>p</i><0.05.</p