An innovative device for determining the soil water retention curve under high suction at different temperatures

To characterise the water retention behaviour of fine soils, high suction values are applied. In this range of values, the vapour equilibrium technique is usually used. This paper presents an innovative device, a sorption bench that permits the determination of the water retention curve of soil. With this new testing method, the time required for testing is significantly reduced. In addition, this apparatus enables the thermal conditions of a test to be controlled; thus, the applied suction can be better controlled, and the water retention curve for different temperatures can be determined. Another valuable aspect of the device is the adopted technical solution that permits weighing of the samples inside the desiccators at any time. Consequently, the water content kinetics can be defined without disturbing the drying or wetting processe

An Innovative Device for Determining the Soil Water Retention Curve Under High Suction at Different Temperatures

In order to characterize the water retention behavior of fine soils, very high suction values have to be applied. In this range of values, the vapor equilibrium technique is usually used. This paper presents an innovative device, a sorption bench, which permits the determination of the water retention curve of soil. By this new testing method, the testing time required is strongly reduced. In addition, this apparatus allows the controlling of the thermal conditions of the test and thus leads to better control of the applied suction as well as to determine the water retention curve for different temperatures. Another important added value of the device is the technical solution adopted, which permits the measurement at any time of the weight of the samples inside the desiccators. Consequently, one can define the water content kinetics without disturbing the drying or wetting processes

Solid-State Hydrogen Storage Systems and the Relevance of a Gender Perspective

This paper aims at addressing the exploitation of solid-state carriers for hydrogen storage, with attention paid both to the technical aspects, through a wide review of the available integrated systems, and to the social aspects, through a preliminary overview of the connected impacts from a gender perspective. As for the technical perspective, carriers to be used for solid-state hydrogen storage for various applications can be classified into two classes: metal and complex hydrides. Related crystal structures and corresponding hydrogen sorption properties are reviewed and discussed. Fundamentals of thermodynamics of hydrogen sorption evidence the key role of the enthalpy of reaction, which determines the operating conditions (i.e., temperatures and pressures). In addition, it rules the heat to be removed from the tank during hydrogen absorption and to be delivered to the tank during hydrogen desorption. Suitable values for the enthalpy of hydrogen sorption reaction for operating conditions close to ambient (i.e., room temperature and 1–10 bar of hydrogen) are close to 30 kJ·molH2−1. The kinetics of the hydrogen sorption reaction is strongly related to the microstructure and to the morphology (i.e., loose powder or pellets) of the carriers. Usually, the kinetics of the hydrogen sorption reaction is rather fast, and the thermal management of the tank is the rate-determining step of the processes. As for the social perspective, the paper arguments that, as it occurs with the exploitation of other renewable innovative technologies, a wide consideration of the social factors connected to these processes is needed to reach a twofold objective: To assess the extent to which a specific innovation might produce positive or negative impacts in the recipient socioeconomic system and, from a sociotechnical perspective, to explore the potential role of the social components and dynamics in fostering the diffusion of the innovation itself. Within the social domain, attention has been paid to address the underexplored relationship between the gender perspective and the enhancement of hydrogen-related energy storage systems. This relationship is taken into account both in terms of the role of women in triggering the exploitation of hydrogen-based storage playing as experimenter and promoter, and in terms of the intertwined impact of this innovation in their current conditions, at work, and in daily life

Down-regulation of the Lamin A/C in neuroblastoma triggers the expansion of tumor initiating cells

Tumor-initiating cells constitute a population within a tumor mass that shares properties with normal stem cells and is considered responsible for therapy failure in many cancers. We have previously demonstrated that knockdown of the nuclear envelope component Lamin A/C in human neuroblastoma cells inhibits retinoic acid-mediated differentiation and results in a more aggressive phenotype. In addition, Lamin A/C is often lost in advanced tumors and changes in the nuclear envelope composition occur during tumor progression. Based on our previous data and considering that Lamin A/C is expressed in differentiated tissues, we hypothesize that the lack of Lamin A/C could predispose cells toward a stem-like phenotype, thus influencing the development of tumor-initiating cells in neuroblastoma. This paper demonstrates that knockdown of Lamin A/C triggers the development of a tumor-initiating cell population with self-renewing features in human neuroblastoma cells. We also demonstrates that the development of TICs is due to an increased expression of MYCN gene and that in neuroblastoma exists an inverse relationship between LMNA and MYCN expression

Unexpected Absence of Skeletal Responses to Dietary Magnesium Depletion: Basis for Future Perspectives?

It's known that a magnesium (Mg)-deficient diet is associated with an increased risk of osteoporosis. The aim of this work is to investigate, by a histological approach, the effects of a Mg-deprived diet on the bone of 8-weeks-old C57BL/6J male mice. Treated and control mice were supplied with a Mg-deprived or normal diet for 8 weeks, respectively. Body weight, serum Mg concentration, expression of kidney magnesiotropic genes, and histomorphometry on L5 vertebrae, femurs, and tibiae were evaluated. Body weight gain and serum Mg concentration were significantly reduced, while a trend toward increase was found in gene expression in mice receiving the Mg-deficient diet, suggesting the onset of an adaptive response to Mg depletion. Histomorphometric parameters on the amount of trabecular and cortical bone, number of osteoclasts, and thickness of the growth plate in femoral distal and tibial proximal metaphyses did not differ between groups; these findings partially differ from most data present in the literature showing that animals fed a Mg-deprived diet develop bone loss and may be only in part explained by differences among the experimental protocols. However, the unexpected findings we recorded on bones could be attributed to genetic differences that may have developed after multiple generations of inbreeding

Il CNR e i risultati della ricerca scientifica Progetti PRIN e FIRB 2007-2013

Il presente volume ha lo scopo di illustrare l'attività della rete scientifica del Consiglio Nazionale delle Ricerche (CNR) nell'ambito delle progettualità PRIN (Progetti di Ricerca di Interesse Nazionale) e FIRB (Fondo per gli Investimenti della Ricerca di Base) promosse dal Ministero dell'Istruzione, dell'Università e della Ricerca (MIUR)

Cognitive deficits and brain myo-Inositol are early biomarkers of epileptogenesis in a rat model of epilepsy

One major unmet clinical need in epilepsy is the identification of therapies to prevent or arrest epilepsy development in patients exposed to a potential epileptogenic insult. The development of such treatments has been hampered by the lack of non-invasive biomarkers that could be used to identify the patients at-risk, thereby allowing to design affordable clinical studies. Our goal was to test the predictive value of cognitive deficits and brain astrocyte activation for the development of epilepsy following a potential epileptogenic injury. We used a model of epilepsy induced by pilocarpine-evoked status epilepticus (SE) in 21-day old rats where 60–70% of animals develop spontaneous seizures after around 70 days, although SE is similar in all rats. Learning was evaluated in the Morris water-maze at days 15 and 65 post-SE, each time followed by proton magnetic resonance spectroscopy for measuring hippocampal myo-Inositol levels, a marker of astrocyte activation. Rats were video-EEG monitored for two weeks at seven months post-SE to detect spontaneous seizures, then brain histology was done. Behavioral and imaging data were retrospectively analysed in epileptic rats and compared with non-epileptic and control animals. Rats displayed spatial learning deficits within three weeks from SE. However, only epilepsy-prone rats showed accelerated forgetting and reduced learning rate compared to both rats not developing epilepsy and controls. These deficits were associated with reduced hippocampal neurogenesis. myo-Inositol levels increased transiently in the hippocampus of SE-rats not developing epilepsy while this increase persisted until spontaneous seizures onset in epilepsy-prone rats, being associated with a local increase in S100β-positive astrocytes. Neuronal cell loss was similar in all SE-rats. Our data show that behavioral deficits, together with a non-invasive marker of astrocyte activation, predict which rats develop epilepsy after an acute injury. These measures have potential clinical relevance for identifying individuals at-risk for developing epilepsy following exposure to epileptogenic insults, and consequently, for designing adequately powered antiepileptogenesis trials

TRAIL-R1 and TRAIL-R2 mediate TRAIL-dependent apoptosis in activated primary human B lymphocytes

The maintenance of B cell homeostasis requires a tight control of B cell generation, survival, activation, and maturation. In lymphocytes upon activation, increased sensitivity to apoptotic signals helps controlling differentiation and proliferation. The death receptor Fas is important in this context because genetic Fas mutations in humans lead to an autoimmune lymphoproliferative syndrome that is similar to lymphoproliferation observed in Fas-deficient mice. In contrast, the physiological role of TNF-related apoptosis-inducing ligand receptors (TRAIL-Rs) in humans has been poorly studied so far. Indeed, most studies have focused on tumor cell lines and on mouse models whose results are difficult to transpose to primary human B cells. In the present work, the expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells. In addition, T-dependent and T-independent signals elicited induction of TRAIL-Rs with distinct kinetics, which differed among B cell subpopulations: switched memory cells rapidly upregulated TRAIL-R1 and -2 upon activation while naïve B cells only reached similar expression levels at later time points in culture. Increased expression of TRAIL-R1 and -2 coincided with a caspase-3-dependent sensitivity to TRAIL-induced apoptosis in activated B cells but not in freshly isolated resting B cells. Finally, both TRAIL-R1 and TRAIL-R2 could signal actively and both contributed to TRAIL-induced apoptosis. In conclusion, this study provides a systematic analysis of the expression of TRAIL-Rs in human primary B cells and of their capacity to signal and induce apoptosis. This dataset forms a basis to further study and understand the dysregulation of TRAIL-Rs and TRAIL expression observed in autoimmune diseases. Additionally, it will be important to foresee potential bystander immunomodulation when TRAIL-R agonists are used in cancer treatment.Fil: Staniek, Julian. Albert Ludwigs University of Freiburg; AlemaniaFil: Lorenzetti, Raquel. Albert Ludwigs University of Freiburg; AlemaniaFil: Heller, Bianca. Albert Ludwigs University of Freiburg; AlemaniaFil: Janowska, Iga. Albert Ludwigs University of Freiburg; AlemaniaFil: Schneider, Pascal. Universite de Lausanne; SuizaFil: Unger, Susanne. Albert Ludwigs University of Freiburg; AlemaniaFil: Warnatz, Klaus. Albert Ludwigs University of Freiburg; AlemaniaFil: Seidl, Maximilian. Albert Ludwigs University of Freiburg; AlemaniaFil: Venhoff, Nils. Albert Ludwigs University of Freiburg; AlemaniaFil: Thiel, Jens. Albert Ludwigs University of Freiburg; AlemaniaFil: Smulski, Cristian Roberto. Comisión Nacional de Energía Atómica. Centro Atómico Bariloche; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Patagonia Norte; ArgentinaFil: Rizzi, Marta. Albert Ludwigs University of Freiburg; Alemani

Il CNR e i risultati della ricerca scientifica_ Progetti PON 2007-2013

Il libro in oggetto raccoglie, sintetizza e presenta i risultati delle attività conseguiti dalla rete scientifica del CNR nell’ambito del Programma Operativo Nazionale “Ricerca e Competitività (PON R&C). Tale programma rappresenta lo strumento attraverso il quale il nostro Paese concorre allo sviluppo della Politica di Coesione della Unione europea a favore delle proprie aree territoriali più svantaggiate. Nel presente volume, si descrivono le caratteristiche generali del PON R&C 2007-2013 e le specifiche tecniche dei cinque bandi emanati dal MIUR: PON Ricerca Industriale (D.D. 1/Ric. del 18/01/2010); PON Distretti ad alta tecnologia e Laboratori pubblico-privati (D.D. 713/Ric. del 29/10/2010); PON Potenziamento Strutturale (D.D. 254/Ric. del 18/05/2011); PON Smart Cities and Communities and Social Innovation (D.D. 84/Ric. del 02/03/2012); PON Cluster Tecnologici Nazionali (D.D. 257/Ric. del 30/05/2012)

Frailty and mortality : Utility of Frail-VIG index in ED short-stay units for older adults

Frailty assessment allows the identification of patients at risk of death. The aim here was to study the ability of Frail-VIG Index (FI-VIG) in order to discriminate frailty groups of older adults and garner its correlation with mortality in an Emergency-Department Short-Stay Unit (ED-SSU). Our observational, single-center, prospective study consecutively included patients over 65-years-old admitted between March 1, 2021, and April 30, 2021. 302 patients were included (56 % women), mean age 83 ± 8 years, and 39.1 % of them had a functional disability whilst 16.5 % of them had dementia. A total of 174 patients (58 %) met the frailty criteria (FI-VIG ≥ 0.2): 111 (63.8 %) had mild frailty (FI-VIG 0.2-0.36), 52 (29.9 %) had moderate frailty (FI-VIG 0.36-0.55), and 11 (6.3 %) had advanced frailty (FI-VIG > 0.55). Mortality at 30 days, 6 months, and 1 year was analyzed: no frailty was 6.3 %, 10.8 %, and 12.5 %, respectively; mild frailty was 10.8 %, 22.5 %, and 22.5 %, respectively; moderate frailty was 25 %, 34.6 %, and 42.3 %, respectively; advanced frailty was 36.4 %, 54.5 %, and 3.6 %, respectively. This shows the significant differences between the groups (1-year mortality p < 0.001). Mild frailty vs. non-frail HR was 2.47 (95 %CI 1.12-5.46), moderate frailty vs. non-frail HR was 6.93 (95 %CI 3.16-15.23), and advanced frailty vs. non-frail HR was 11.29 (95 %CI 3.54-36.03). The mean test time was 7 min. There was a strong correlation between frailty degree and mortality at 1, 6, and 12 months. FI-VIG is fast and easy-to-use in this setting. It is routine implementation in ED-SSUs could enable early risk stratification