Development and Evaluation of Cefadroxil Drug Loaded Biopolymeric Films Based on Chitosan-Furfural Schiff Base

Cefadroxil drug loaded biopolymeric films of chitosan-furfural schiff base were prepared by reacting chitosan with furfural in presence of acetic acid and perchloric acid respectively for the external use. Prepared films were evaluated for their strength, swelling index, thickness, drug content, uniformity, tensile strength, percent elongation, FTIR spectral analysis and SEM. The results of in vitro diffusion studies revealed that the films exhibited enhanced drug diffusion as compared to the films prepared using untreated chitosan. The films also demonstrated good to moderate antibacterial activities against selective gram positive and gram negative bacteria

DNA-Binding Interaction Studies of Microwave Assisted Synthesized Sulfonamide Substituted 8-Hydroxyquinoline Derivatives

Sulfonamide substituted 8-hydroxyquinoline derivatives were prepared using a microwave synthesizer. The interaction of sulfonamide substituted 8-hydroxyquinoline derivatives and their transition metal complexes with Plasmid (pUC 19) DNA and Calf Thymus DNA were investigated by UV spectroscopic studies and gel electrophoresis measurements. The interaction between ligand/metal complexes and DNA was carried out by increasing the concentration of DNA from 0 to 12 μl in UV spectroscopic study, while the concentration of DNA in gel electrophoresis remained constant at 10 μl. These studies supported the fact that, the complex binds to DNA by intercalation via ligand into the base pairs of DNA. The relative binding efficacy of the complexes to DNA was much higher than the binding efficacy of ligands, especially the complex of Cu-AHQMBSH had the highest binding ability to DNA. The mobility of the bands decreased as the concentration of the complex was increased, indicating that there was increase in the interaction between the metal ion and DNA. Complexes of AHQMBSH were excellent for DNA binding as compared to HQMABS

High yield synthesis of electrolyte heating assisted electrochemically exfoliated graphene for electromagnetic interference shielding applications

Herein, we demonstrate a facile one pot synthesis of graphene nanosheets by electrochemical exfoliation of graphite. In the present study, we report a significant increase in the yield of graphene by electrolyte heating assisted electrochemical exfoliation method. The obtained results of heating assisted electrochemically exfoliated graphene (utilizing H2SO4 + KOH + DW) synthesis clearly exhibit that the yield increases similar to 4.5 times i.e. from similar to 17% (room temperature) to similar to 77% (at 80 degrees C). A plausible mechanism for the enhanced yield based on lattice expansion and vibration of intercalated ions has been put forward and discussed in details. The quality of graphene was examined by Raman, XPS, FTIR, AFM, SEM, TEM/HRTEM and TGA techniques. The Raman as well as morphogenesis results confirm the quality of the graphene nanosheets. We have used this graphene as electromagnetic interference shielding material where a comparatively large quantity of graphene is required. This graphene exhibits enhanced shielding effectiveness (46 dB at 1 mm thickness of stacked graphene sheets in frequency region 12.4 to 18 GHz) as compared to conventional electromagnetic interference shielding materials, which is greater than the recommended limit (similar to 30 dB) for techno-commercial applications. Thus the present work is suggestive for future studies on enhancement of yield of high quality graphene by proposed method and the use of synthesized graphene in electromagnetic interference shielding and other possible applications

Ageratum enation virus Infection Induces Programmed Cell Death and Alters Metabolite Biosynthesis in Papaver somniferum

A previously unknown disease which causes severe vein thickening and inward leaf curl was observed in a number of opium poppy (Papaver somniferum L.) plants. The sequence analysis of full-length viral genome and associated betasatellite reveals the occurrence of Ageratum enation virus (AEV) and Ageratum leaf curl betasatellite (ALCB), respectively. Co-infiltration of cloned agroinfectious DNAs of AEV and ALCB induces the leaf curl and vein thickening symptoms as were observed naturally. Infectivity assay confirmed this complex as the cause of disease and also satisfied the Koch’s postulates. Comprehensive microscopic analysis of infiltrated plants reveals severe structural anomalies in leaf and stem tissues represented by unorganized cell architecture and vascular bundles. Moreover, the characteristic blebs and membranous vesicles formed due to the virus-induced disintegration of the plasma membrane and intracellular organelles were also present. An accelerated nuclear DNA fragmentation was observed by Comet assay and confirmed by TUNEL and Hoechst dye staining assays suggesting virus-induced programmed cell death. Virus-infection altered the biosynthesis of several important metabolites. The biosynthesis potential of morphine, thebaine, codeine, and papaverine alkaloids reduced significantly in infected plants except for noscapine whose biosynthesis was comparatively enhanced. The expression analysis of corresponding alkaloid pathway genes by real time-PCR corroborated well with the results of HPLC analysis for alkaloid perturbations. The changes in the metabolite and alkaloid contents affect the commercial value of the poppy plants

Mapping the Research output of Bundelkhand University: A Bibliometric Analysis of Publications from 2000-2019

Measuring the productivity and output is a common practice for the institutions, when we discuss about research and publications. Keeping this in mind, the bibliometric analysis of Bundelkhand University, Jhansi from the year 2000-2019 is performed in the given paper. This study shows the growth of Bundelkhand University in terms of Primary research output and publicaions in all disciplines. The total number of publications (579) indexed in SCOPUS were retrieved and analyzed to find out the year wise growth, authorship pattern, collaborative coefficient, prolific authors and popular journals for communicating the research work. MS-Excel is used for analyzing and sorting the retrieved data. Collaborative coefficient is calculated by using the formula defined by Price & Beaver. The results of the study show that the year 2019 was the year of maximum publications (57). The highest citations were received in the year 2005 (1091) and ACCP for total number of publications was 10.71. The maximum publications published as journal articles and three-authorship pattern is dominating other patterns. Dr. S.P. Singh from department of Pharmacy was found as the most prolific author

Development and Evaluation of Cefadroxil Drug Loaded Biopolymeric Films Based on Chitosan-Furfural Schiff Base

Cefadroxil drug loaded biopolymeric films of chitosan-furfural schiff base were prepared by reacting chitosan with furfural in presence of acetic acid and perchloric acid respectively for the external use. Prepared films were evaluated for their strength, swelling index, thickness, drug content, uniformity, tensile strength, percent elongation, FTIR spectral analysis and SEM. The results of in vitro diffusion studies revealed that the films exhibited enhanced drug diffusion as compared to the films prepared using untreated chitosan. The films also demonstrated good to moderate antibacterial activities against selective gram positive and gram negative bacteria

Formulation and In Vitro Evaluation of Ofloxacin Tablets using Natural Gums as Binders

Natural gums are economical, easily available, and useful as tablet binders. In the present investigation, an attempt was made to formulate Ofloxacin tablets using three natural binders, namely Acacia arabica, Hibiscus esculentus, and xanthan gum. Such six batches of Ofloxacin tablets were prepared by using different types and amounts of the natural binders by the wet granulation method. The tablets were analyzed for their hardness, friability, and weight variation, and in vitro release was performed in a phosphate buffer at pH 6.8. The prepared tablets were also evaluated for their various release kinetics and similarity factors f2. The physical properties of the tablets containing the natural binders showed sufficient hardness, desirable disintegration time, and low friability. Their better percentage of drug release was observed as compared to the marketed formulation showing more than 85% drug release within 45 minutes. The in vitro release data was well-fitted into zero-order and the values of release exponent ‘n’ were between 0.303 and 0.514. The high similarity factor f2 of 64.50 was achieved with the best batch in comparison to the marketed tablets. The results obtained indicated that the gum Acacia arabica performed as well as gelatin compared to the other binders for the Ofloxacin tablet formulation

Effect of Shashtika Shali Pinda Sweda & other Ayurvedic Intervention in Cerebral palsy: A Case Report

Cerebral palsy is a disorder that affects muscle tone, movement and motor skills (the ability to move in a coordinated and purposeful way). Cerebral Palsy cannot be correlated with any single disease or condition mentioned in Ayurveda, as it is a multi-factorial disease. The classical signs and symptoms of CP will fit into criteria of Vata Vyadhi spectrum (Vata predominant disease) like Ekangvata, Sarvangvata, Pakshaghata, Pangu, Kampavata etc and Phakkaroga. Although cerebral palsy cannot be completely cured but treatment will generally enhance a child’s abilities. Agnimandya, Amavastha and Kaphavastha should be considered while planning the line of treatment in CP cases. The selected Ayurvedic treatment modality is highly effective in relieving the signs and symptoms and thus reducing the disability in children with CP. Swedana (sudation) is the therapy that relieves the Stambha (stiffness) of the body, mitigate feeling of Guruta (heaviness) and Sheeta (feeling of cold). Shastika shali panda sweda comes under the category of Saagniseda with Snigda dravya as milk and Shalidhanya. It has Snigdha, Guru, Sthira, Sheeta, Tridoshaghna and Brimhanaguna. In this article, an attempt to treat a child with spastic diplegia using multiple Ayurveda treatment modalities. At the end of one year of treatment, Panchkarma procedures along with internal medication resulted in 15-20% improvement in the overall effect of therapy

Synthesis and dyeing properties of new disazo disperse dyes for polyester and nylon fabrics

Diazotized aryl amines were coupled with two stenhouse salt namely, N-(5-phenylamino-penta-4-ol-2,4-diene-1-ylidene)anilines hydrochloride [S1 (RH) or S2 (ROH)] to furnish two series of disazo disperse dyes (S1D1–10 and S2D1–10). The structure of all the dyes was established by estimating number of azo groups, elemental analysis and spectral studies (IR, 1H-NMR, UV/Visible). The structure–property relationship was discussed by using electronic absorption spectra of the dyes. These dyes were applied to polyester and nylon fabrics as disperse dyes by using temperature exhaust dyeing method. The relevant dyeing characteristics, such as dyeability on fabrics, wash-fastness and light-fastness were evaluated. Fabrics dyed with these dyes furnished generally deep and bright intense hues ranging from light yellow to orange to reddish brown. The color fastness of the dyed fabric was assessed by determining wash-fastness and light-fastness properties