Acoustic Response of a Vibrating Elongated Cylinder in a Hydrodynamic Turbulent Flow

The present paper contains the results of the numerical analysis of the interaction between a Newtonian incompressible turbulent flow and a linear elastic slender body, together with the influence of the fluid-structure interaction (FSI) on the noise generation and propagation. The purpose is to evaluate the differences in term of acoustic pressure between the case where the solid body is rigid (infinite stiffness) and the case where it is elastic (finite stiffness). A partitioned and implicit algorithm with the arbitrary Lagrangian-Eulerian method (ALE) is used for the interaction between the fluid and solid. For the evaluation of the turbulent fluid motion, we use a large eddy simulation (LES) with the Smagorinsky subgrid scale model. The equation for the solid is solved through the Lagrangian description of the momentum equation and the second Piola-Kirchoff stress tensor. In addition, the acoustic analogy of Lighthill is used to characterize the acoustic source (the slender body) by directly using the fluid dynamic fields. In particular, we use the Ffowcs Williams and Hawkings (FW-H) equation for the evaluation of the acoustic pressure in the fluid medium. As a first numerical experiment, we analyze a square cylinder immersed in a turbulent flow characterized by two different values of stiffness: one infinite (rigid case) and one finite (elastic case). In the latter case, the body stiffness and mean flow velocity are such that they induce the lock-in phenomenon. Finally, we evaluate the differences in terms of acoustic pressure between the two different cases

Establishing Human Lacrimal Gland Cultures with Secretory Function

PURPOSE: Dry eye syndrome is a multifactorial chronic disabling disease mainly caused by the functional disruptions in the lacrimal gland. The treatment involves palliation like ocular surface lubrication and rehydration. Cell therapy involving replacement of the gland is a promising alternative for providing long-term relief to patients. This study aimed to establish functionally competent lacrimal gland cultures in-vitro and explore the presence of stem cells in the native gland and the established in-vitro cultures. METHODS: Fresh human lacrimal gland from patients undergoing exenteration was harvested for cultures after IRB approval. The freshly isolated cells were evaluated by flow cytometry for expression of stem cell markers ABCG2, high ALDH1 levels and c-kit. Cultures were established on Matrigel, collagen and HAM and the cultured cells evaluated for the presence of stem cell markers and differentiating markers of epithelial (E-cadherin, EpCAM), mesenchymal (Vimentin, CD90) and myofibroblastic (α-SMA, S-100) origin by flow cytometry and immunocytochemistry. The conditioned media was tested for secretory proteins (scIgA, lactoferrin, lysozyme) post carbachol (100 µM) stimulation by ELISA. RESULTS: Native human lacrimal gland expressed ABCG2 (mean±SEM: 3.1±0.61%), high ALDH1 (3.8±1.26%) and c-kit (6.7±2.0%). Lacrimal gland cultures formed a monolayer, in order of preference on Matrigel, collagen and HAM within 15-20 days, containing a heterogeneous population of stem-like and differentiated cells. The epithelial cells formed 'spherules' with duct like connections, suggestive of ductal origin. The levels of scIgA (47.43 to 61.56 ng/ml), lysozyme (24.36 to 144.74 ng/ml) and lactoferrin (32.45 to 40.31 ng/ml) in the conditioned media were significantly higher than the negative controls (p<0.05 for all comparisons). CONCLUSION: The study reports the novel finding of establishing functionally competent human lacrimal gland cultures in-vitro. It also provides preliminary data on the presence of stem cells and duct-like cells in the fresh and in-vitro cultured human lacrimal gland. These significant findings could pave way for cell therapy in future

Current status and trends of biological invasions in the Lagoon of Venice, a hotspot of marine NIS introductions in the Mediterranean Sea

This paper provides an updated account of the occurrence and abundance of non-indigenous species (NIS) in an area of high risk of introduction: the Lagoon of Venice (Italy). This site is a known hotspot of NIS introductions within the Mediterranean Sea, hosting all the most important vectors of introduction of marine NIS—shipping, recreational boating, shellfish culture and live seafood trade. The recent literature demonstrates that the number of NIS in Venice is continuously changing, because new species are being introduced or identified, and new evidence shows either an exotic origin of species previously believed to be native, or a native origin of formerly believed ‘‘aliens’’, or demonstrates the cryptogenic nature of others. The number of NIS introduced in the Venetian lagoon currently totals 71, out of which 55 are established. This number exceeds those displayed by some nations like Finland, Portugal or Libya. Macroalgae are the taxonomic group with the highest number of introduced species (41 % of NIS): the most likely vector for their introduction is shellfish culture. The source region of NIS introduced to Venice is mainly represented by other Mediterranean or European sites (76 %). The Lagoon of Venice represents a sink but also a source of NIS in the Mediterranean Sea, as it is the site of first record of several NIS, which have since further spread elsewhere.This paper provides an updated account of the occurrence and abundance of non-indigenous species (NIS) in an area of high risk of introduction: the Lagoon of Venice (Italy). This site is a known hotspot of NIS introductions within the Mediterranean Sea, hosting all the most important vectors of introduction of marine NIS-shipping, recreational boating, shellfish culture and live seafood trade. The recent literature demonstrates that the number of NIS in Venice is continuously changing, because new species are being introduced or identified, and new evidence shows either an exotic origin of species previously believed to be native, or a native origin of formerly believed "aliens", or demonstrates the cryptogenic nature of others. The number of NIS introduced in the Venetian lagoon currently totals 71, out of which 55 are established. This number exceeds those displayed by some nations like Finland, Portugal or Libya. Macroalgae are the taxonomic group with the highest number of introduced species (41 % of NIS): the most likely vector for their introduction is shellfish culture. The source region of NIS introduced to Venice is mainly represented by other Mediterranean or European sites (76 %). The Lagoon of Venice represents a sink but also a source of NIS in the Mediterranean Sea, as it is the site of first record of several NIS, which have since further spread elsewhere

Vegetation and fauna: essential bio-indicators for the asessment of agro-ecosystems

One of the most important scientific contributions to the application of the Landascape Ecology’s disciplines is represented by the development of indicators to assess environmental quality. In particular, the use of bio-indicators based on flora, vegetation and fauna allowed a better classification of threatened species and habitats, together with a development of specific environmental policies. Despite this, many of the analysis and methods used in several conservation actions showed some important application limits, especially inside modified ecosystems such as farmlands and urban areas. The importance of agro-ecosystems for the conservation of the whole territory has been universally recognized by the most part of scientific and productive sectors. Nonetheless, there is still a lack of knowledge, methods and experimental trials able to provide a sensitive assessment of actual environmental conditions, and of all corrective actions to be taken. This work presents a method to assess environmental quality inside agro-ecosystems; it is based on a system of bio-indicators used as an analysis tool able to take advantage of all potential information brought by different bio-coenosis. Starting from the innovations of the phytosociological dynamic approach, the main objective is to take into account the results provided by vegetational analysis, concerning the description of land units and phyto-coenotic mosaics, and to improve their information capacity through an integration with quantitative data. These data can be obtained by the application of specific vegetation and faunistic bio-indicators created to be used inside agro-ecosystems. Essential faunistic bio-indicators were chosen for their sensitiveness and suitability inside different application contexts; they can also allow to focus on specific environmental factors undetectable with the vegetation analysis, such as the responses of different keystone species to environmental fragmentation and to insecticides. This kind of interpretation of landscape dynamics, especially inside agro-ecosystems, is coherent with the application of European Agro-environmental Policies (CAP, RDP, Habitat Dir.), and with the main objective of biodiversity conservation (Countdown 2010). The importance of a knowledge base for the assessment of policies effectiveness is also necessary to evaluate sustainable systems for environmental management inside both natural (protected areas) and agricultural systems

A Rare Presentation of Multiple Sclerosis without Optic Neuritis

Multiple Sclerosis is a well known demyelinating disease commonly associated with periventricular plaques on MRI and painful optic neuritis. Many patients also present with visual field defects, most commonly diffuse loss or central defects in the affected eye