409 research outputs found
Finite element study of the accommodation behaviour of the crystalline lens after fs-laser treatment
With aging the ability of the crystalline lens to adapt to different viewing distances decreases. Until now there is no satisfying treatment available. It is possible to influence the deformability of the crystalline lens by inducing cuts using femtosecond (fs)-laser. To test the influence of several different cutting geometries is expensive and a huge amount of crystalline lenses is needed. Finit Element Method offers the possibility to test the influence of different cutting geometries on the flexibility. Therefore, it is necessary to develop a method to simulate the cuts in an adequate way. In first simulations cuts were assumed to be isotropic inter layers. Later on, effective material properties for the inter layer were calculated with a homogenization technique. The results confirm the influence of the cuts on the flexibility.DFG/BE3990-
Three-dimensional imaging of intracochlear tissue by scanning laser optical tomography (SLOT)
The presented study focuses on the application of scanning laser optical tomography (SLOT) for non-destructive visualization of anatomical structures inside the human cochlea ex vivo. SLOT is a laser-based highly efficient microscopy technique, which allows for tomographic imaging of the internal structure of transparent large-scale specimens (up to 1 cm3). Thus, in the field of otology this technique is best convenient for an ex vivo study of the inner ear anatomy. For this purpose, the preparation before imaging comprises mechanically assisted decalcification, dehydration as well as optical clearing of the cochlea samples. Here, we demonstrate results of SLOT visualizing hard and soft tissue structures of the human cochlea with an optical resolution in the micrometer range using absorption and autofluorescence as contrast mechanisms. Furthermore, we compare our results with the method of X-ray micro tomography (micro-CT, μCT) as clinical gold standard which is based only on absorption. In general, SLOT can provide the advantage of covering all contrast mechanisms known from other light microscopy techniques, such as fluorescence or scattering. For this reason, a protocol for antibody staining has been developed, which additionally enables selective mapping of cellular structures within the cochlea. Thus, we present results of SLOT imaging rodent cochleae showing specific anatomical structures such as hair cells and neurofilament via fluorescence. In conclusion, the presented study has shown that SLOT is an ideally suited tool in the field of otology for in toto visualization of the inner ear microstructure. © 2016 SPIE
Microbial arms race: Ballistic "nematocysts" in dinoflagellates represent a new extreme in organelle complexity
We examine the origin of harpoon-like secretory organelles (nematocysts) in dinoflagellate protists. These ballistic organelles have been hypothesized to be homologous to similarly complex structures in animals (cnidarians); but we show, using structural, functional, and phylogenomic data, that nematocysts evolved independently in both lineages. We also recorded the first high-resolution videos of nematocyst discharge in dinoflagellates. Unexpectedly, our data suggest that different types of dinoflagellate nematocysts use two fundamentally different types of ballistic mechanisms: one type relies on a single pressurized capsule for propulsion, whereas the other type launches 11 to 15 projectiles froman arrangement similar to a Gatling gun.Despite their radical structural differences, these nematocysts share a single origin within dinoflagellates and both potentially use a contraction-based mechanism to generate ballistic force. The diversity of traits in dinoflagellate nematocysts demonstrates a stepwise route by which simple secretory structures diversified to yield elaborate subcellular weaponry
The Chemical Composition and Gas-to-Dust Mass Ratio of Nearby Interstellar Matter
Recent results on nearby interstellar gas and interstellar byproducts within
the solar system are used to select among the equilibrium radiative transfer
models of the nearest interstellar material of Slavin and Frisch (2002).
Assuming O/H~400 ppm, Models 2 and 8 are found to yield good fits to available
data on local interstellar material, and pickup ions and anomalous cosmic rays
inside of the heliosphere, with the exception of Ne. For these models, the
density of interstellar gas at the entry point to the heliosphere is n(HI)~0.20
/cc and n(e-)~0.1 /cc. These models suggest the chemical composition of the
nearby ISM is ~60--70% subsolar if S is undepleted (where HI and HII must be
included for abundance calculations). Gas-to-dust mass ratios of 178-183 for
solar abundances, or 611-657 for 70% solar abundances are found. The percentage
of the dust mass that is carried by iron is directly correlated with the
gas-to-dust mass ratio implying an Fe-rich grain core remains after grain
destruction.Comment: 36 pages, 6 figures, accepted by Ap
Origin of Shifts in the Surface Plasmon Resonance Frequencies for Au and Ag Nanoparticles
Origin of shifts in the surface plasmon resonance (SPR) frequency for noble
metal (Au, Ag) nanoclusters are discussed in this book chapter. Spill out of
electron from the Fermi surface is considered as the origin of red shift. On
the other hand, both screening of electrons of the noble metal in porous media
and quantum effect of screen surface electron are considered for the observed
blue shift in the SPR peak position.Comment: 37 pages, 14 Figures in the submitted book chapter of The Annual
Reviews in Plasmonics, edited by Professor Chris D. Geddes. Springer Scinec
The unidentified TeV source (TeVJ2032+4130) and surrounding field: Final HEGRA IACT-System results
The unidentified TeV source in Cygnus is now confirmed by follow-up
observations from 2002 with the HEGRA stereoscopic system of Cherenkov
Telescopes. Using all data (1999 to 2002) we confirm this new source as steady
in flux over the four years of data taking, extended with radius 6.2 arcmin
(+-1.2 arcmin (stat) +-0.9 arcmin (sys)) and exhibiting a hard spectrum with
photon index -1.9. It is located in the direction of the dense OB stellar
association, Cygnus OB2. Its integral flux above energies E>1 TeV amounts to
\~5% of the Crab assuming a Gaussian profile for the intrinsic source
morphology. There is no obvious counterpart at radio, optical nor X-ray
energies, leaving TeVJ2032+4130 presently unidentified. Observational
parameters of this source are updated here and some astrophysical discussion is
provided. Also included are upper limits for a number of other interesting
sources in the FoV, including the famous microquasar Cygnus X-3.Comment: 7 pages, 3 figures. Accepted for publication in Astronomy &
Astrophysic
Observations of H1426+428 with HEGRA -- Observations in 2002 and reanalysis of 1999&2000 data
The HEGRA system of imaging air Cherenkov telescopes has been used to observe
the BL Lac object H1426+428 () for 217.5 hours in 2002. In this data
set alone, the source is detected at a confidence level of ,
confirming this object as a TeV source. The overall flux level during the
observations in 2002 is found to be a factor of lower than during
the previous observations by HEGRA in 1999&2000. A new spectral analysis has
been carried out, improving the signal-to-noise ratio at the expense of a
slightly increased systematic uncertainty and reducing the relative energy
resolution to over a wide range of energies. The new
method has also been applied to the previously published data set taken in 1999
and 2000, confirming the earlier claim of a flattening of the energy spectrum
between 1 and 5 TeV. The data set taken in 2002 shows again a signal at
energies above 1 TeV. We combine the energy spectra as determined by the CAT
and VERITAS groups with our reanalyzed result of the 1999&2000 data set and
apply a correction to account for effects of absorption of high energy photons
on extragalactic background light in the optical to mid infrared band. The
shape of the inferred source spectrum is mostly sensitive to the
characteristics of the extragalactic background light between wavelengths of 1
and 15~mComment: 12 pages, 4 Figures, submitted to A&
Is the giant radio galaxy M 87 a TeV gamma-ray emitter?
For the first time an excess of photons above an energy threshold of 730 GeV from the giant radio galaxy M 87 has been measured at a significance level above 4 σ. The data have been taken during the years 1998 and 1999 with the HEGRA stereoscopic system of 5 imaging atmospheric Cherenkov telescopes. The excess of 107.4 ± 26.8 events above 730 GeV corresponds to an integral flux of 3.3% of the Crab flux or Nγ (E > 730 GeV) = (0.96 ± 0.23) × 10-12 phot cm-2 s-1. M 87 is located at the center of the Virgo cluster of galaxies at a relatively small redshift of z = 0.00436 and is a promising candidate among the class of giant radio galaxies for the emission of TeV γ-radiation. The detection of TeV γ-rays from M 87 - if confirmed - would establish a new class of extragalactic source in this energy regime since all other AGN detected to date at TeV energies are BL Lac type objects.F. A. Aharonian ...G. P. Rowell...et al
A search for TeV gamma-ray emission from SNRs, pulsars and unidentified GeV sources in the Galactic plane in the longitude range between -2 deg and 85 deg
Using the HEGRA system of imaging atmospheric Cherenkov telescopes, one
quarter of the Galactic plane (-2 deg < l < 85 deg) was surveyed for TeV
gamma-ray emission from point sources and moderately extended sources (diameter
<= 0.8 deg). The region covered includes 86 known pulsars (PSR), 63 known
supernova remnants (SNR) and nine GeV sources, representing a significant
fraction of the known populations. No evidence for emission of TeV gamma
radiation was detected, and upper limits range from 0.15 Crab units up to
several Crab units, depending on the observation time and zenith angles
covered. The ensemble sums over selected SNR and pulsar subsamples and over the
GeV-sources yield no indication for emission from these potential sources. The
upper limit for the SNR population is at the level of 6.7% of the Crab flux and
for the pulsar ensemble at the level of 3.6% of the Crab flux.Comment: 10 pages, 5 figures, 4 tables, accepted for publication in A&
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