Captain Wayne P. Hughes, Jr., USN (Ret.) interviewed by Colonel Paul T. Ringenbach, USAF (Ret.) June 1, 2001

A transcript of an interview of Captain Wayne P. Hughes USN (Ret.) USNA ‘52 conducted by Colonel Paul T. Ringenbach (Ret.) USAF. A cover letter from Colonel Ringenbach, and a signed release from Captain Hughes are included. This inverview has been shared by Special Collections & Archives, Nimitz Library, U.S. Naval Academy

Architecture and orogenic evolution of the northeastern Outer Carpathians from cross-section balancing and forward modeling

New balanced and restored cross-sections and a 2D kinematic model illustrate the present geometry of the northeastern Outer Carpathians and quantify their orogenic evolution between the late Eocene and the late Miocene (~. 35.3 to ~. 11.0. Ma). The balanced cross-section is built on extensive surface and subsurface data and depicts an imbricate fan internally stacked along high-displacement out-of-sequence thrusts. Section restoration yielded 507. km of minimum orogenic shortening - at least ~. 230. km more than proposed in previous studies. Our shortening estimate relies on accurate thicknesses of lithostratigraphic units, in most cases thinner than applied before. The average convergence rate between ~. 35.3 and ~. 11.0. Ma is estimated at 20.8. km/My. The forward model, constrained by lower and upper ages of syn-orogenic deposits, traces the advance of the Outer Carpathian accretionary wedge and proves kinematic admissibility of the balanced and restored cross-sections. Progradation rates of the foreland basin depositional sequences, evolving from 9-11. km/My between ~. 41.5 and ~. 21.1. Ma to 22-39. km/My between ~. 21.1 and ~. 11.0. Ma, suggest a two-phase dynamics of the orogenic system. The acceleration of foreland migration at ~. 21.1. Ma likely reflects the onset of northward subduction retreat, linked to the rollback of the European lower plate that gave rise to coeval back-arc extension in the Pannonian Basin. © 2012 Elsevier B.V..This study is a contribution of the Group of Dynamics of the Lithosphere (GDL) within the frame of a collaborative research project on fold-and-thrust belts with TOTAL® and the Polish Geological Institute National Research Institute. We thank TOTAL® for permission to publish this work. We also thank partial support from TopoMed CGL2008-03474-E/BTE and Consolider-Ingenio 2010 Topo-Iberia (CSD2006-00041) projects and to N. Oszczypko for introducing us to the geology of the research area in the Polish Carpathians.Peer Reviewe

Forward Model of the Eocene-Miocene Orogenic Evolution of Polish and Slovak Outer Carpathians

72nd EAGE Conference & Exhibition incorporating SPE EUROPEC 2010, Barcelona, Spain, 14 - 17 June 2010The Polish and Slovak Outer Carpathians are the Eocene-Miocene accretionary wedge resting upon flexed margin of the European Plate. Forward modeling has shown that the its kinematics was controlled not only by frontal accretion on new thrust sheets but also by thickening of its internal parts driven by out-ofsequence thrusting. Growth and arrival of the accretionary wedge to its present position resulted from at least 495 km of orogenic convergence. Rates of convergence were evolving from 10.5 mm/y between 35.2 and 25.0 Ma to 24.2 mm/y from 25.0 Ma onwards. This acceleration may be attributed to the onset of rollback of the lower plate.The research has been inspired and supported by TOTAL®. We are grateful for donation of data and permission to publish the results

Whole-exome sequencing improves the diagnosis yield in sporadic infantile spasm syndrome

International audienceInfantile spasms syndrome (ISs) is characterized by clinical spasms with ictal electrodecrement, usually occurring before the age of 1 year and frequently associated with cognitive impairment. Etiology is widely heterogeneous, the cause remaining elusive in 40% of patients. We searched for de novo mutations in 10 probands with ISs and their parents using whole-exome sequencing (WES). Patients had neither consanguinity nor family history of epilepsy. Common causes of ISs were excluded by brain magnetic resonance imaging (MRI), metabolic screening, array-comparative genomic hybridization (CGH) and testing for mutations in CDKL5, STXBP1, and for ARX duplications. We found a probably pathogenic mutation in four patients. Missense mutations in SCN2A (p.Leu1342Pro) and KCNQ2 (p.Ala306Thr) were found in two patients with no history of epilepsy before the onset of ISs. The p.Asn107Ser missense mutation of ALG13 had been previously reported in four females with ISs. The fourth mutation was an in-frame deletion (p.Phe110del) in NR2F1, a gene whose mutations cause intellectual disability, epilepsy, and optic atrophy. In addition, we found a possibly pathogenic variant in KIF3C that encodes a kinesin expressed during neural development. Our results confirm that WES improves significantly the diagnosis yield in patients with sporadic ISs

Influence of murine hepatitis induced by D-(+)-galactosamine hydrochloride and lipopolysaccharide on gene expression of polyethylenimine/plasmid DNA polyplex

We investigated the influence of murine hepatitis induced by D-(+)-galactosamine and lipopolysaccharide (DGalN/LPS) on polyethylenimine (PEI)-mediated plasmid DNA (pDNA) delivery. pDNA encoding firefly luciferase was used as the model reporter gene. PEI was used as the non-viral vector because of its high gene expression and low toxicity. The activities of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in mice indicated the highest peaks at 12 h after D-GalN/LPS injection, then the activities of serum ALT and AST rapidly decreased. We determined luciferase activity in various organs of D-GalN/LPS-treated mice and control mice after an intravenous administration of PEI/pDNA complexes. High transgene expression was observed in the liver, spleen, and lung of both mice. Compared to the control mice, a significant increase of transgene expression was observed in the liver of D-GalN/LPS-treated mice after D-GalN/LPS injection. The transgene expression in the spleen and lung decreased at 6 and 12 h after D-GalN/LPS injection. In conclusion, we found that murine hepatitis induced by D-GalN/LPS injection can influence PEI-mediated pDNA delivery and its influence was different from that induced by CCl4 injection which was reported previously. These results demonstrated the necessity of considering the timing and dose of gene therapy according to the disease and its stage