PERUBAHAN MORFOLOGIK DAN FUNGSIONAL JARlNGAN MUKOSA RONGGA MULUT SEBAGAI LANDASAN GIGI TIRUAN LENGKAP (KAJlAN PUSTAKA)

Peningkatan keratinisasi karena pemakaian GTL pada awalnya merupakan proses adaptasi rongga mulut namun, jika tidak dikontrol dengan seksama dapat berdampak negatip yang dapat berkembang ke arah keganasan dan kelainan oklusi yang para

EFEK PEMBERIAN MINYAK ATSIRI KULIT BUAH LEMON (Citrus limon L.) TERHADAP SITOMORFOMETRIK Candida albicans

Background :The most commonly fungal infections found in the oral cavity are oral candidiasis which largely caused by Candida species, particularly Candida albicans. Candida infection increase because its difficult to treat and associated with antifungal drugs resistance. These prompt the researchers to continue develop new drugs and compounds as well as alternative therapies including from natural sources which have antifungal activity to treat fungal infections. Allegedly, components limonene, β-pinene and ɣ -terpinene contained in Citrus limon essential oil has quite good antifungal activity against Candida albicans. Objectives : The aim of this research was to examine and analyze the effect of Citrus limon essential oils on Candida albicans cytomorphometrics. Method : The research used post test only controls group design. Antifungal activity result of the pre-elementary study subdivided into 1.56%, 1.37%, 1.17%, 0.98% and 0.78% of concentration Citrus limon essential oil by Candida albicans inoculum and incubated for 24 hours and 48 hours. After incubation Candida albicans were fixed and dried then observed used scanning electron microscope. Result :Effective concentration of Citrus limon essential oils which affect cytomorphometric of Candida albicans cells at 1.37% and 1.56% concentrations with 48 hours incubation period. Conclusion : Candida albicans undergoing necrosis process through cytomorphometric changes after administration Citrus limon essential oil at a concentration of 1.56% and 1.37% with 48 hours incubation period. Key words : Citrus limon, Candida albicans, necrosis, cytomorphometri

Effects of citrus limon essential oil (Citrus limon L.) on cytomorphometric changes of Candida albicans

Background: The most common fungal infection found in oral cavity is oral candidiasis, largely caused by Candida species, particularly Candida albicans (C. albicans). Candida infection can get worse since it is difficult to be treated and resistant with antifungal drugs. Therefore, new drugs and compounds as well as alternative therapies involving natural sources that have antifungal activities have continually been developed. Limonene, β-pinene, and ɣ-terpinene contained in Citrus limon essential oil have been known to have quite good antifungal activities against C. albicans. Purpose: This research aimed to examine and analyze the effects of Citrus limon essential oil on cytomorphometric changes of C. albicans. Method: The research used post test only control group design. Based on the results of the pre-elementary research on antifungal activities of Citrus limon essential oil against C. albicans, Citrus limon essential oil used in this research was on concentrations of 1.56%, 1.37%, 1.17%, 0.98%, and 0.78%. Citrus limon essential oil by C. albicans inoculum and incubated for 24 hours and 48 hours. After the incubation, those C. albicans cells were fixed, dried, and then observed using a scanning electron microscopy. Result: The most effective concentrations of Citrus limon essential oil triggering cytomorphometric changes of Candida albicans were at 1.37% and 1.56% with the incubation period of 48 hours. Conclusion: C. albicans can undergo necrosis process through cytomorphometric changes after the administration of Citrus limon essential oil at concentrations of 1.56% and 1.37% with the incubation period of 48 hours