乳幼児をもつ勤労女性の夜間の育児による睡眠中断の状況

本研究は,乳幼児を持つ勤労女性の夜間の育児による睡眠中断の状況を,携帯用活動量測定器アクチウォッチを用いて計測した活動量および睡眠日誌から検討した。その結果,以下のことが明らかになった。1.睡眠とみなされる時間は著しく少なくはなかったが,睡眠中断が頻回にみられ睡眠効率が悪い。2.育児による睡眠中断の際,彼女らはほとんど無意識でこれらを行っている。3.就労日・休日を問わず,睡眠中断があることによって,十分な休息をとることが困難である。The purpose of this study was to investigate the quality of sleep who have to take care of infants. The subjects were two women who are working in daytime and taking care of infants during nights. Sleep logs and actiwatch activities were evaluated during 3 consecutive nights. The results were as follows 1. Altough time in bed was enough, there are many waking times with low sleep efficiency. 2. They took care of children several times during nights, but they could not remenber all of them in the next morning. 3. Even if in the weekend, they could not take enough sleep time, because of their interrupted sleep

遠赤外線素材のソックスにおける保温効果の検討 : ポリエステル素材ソックスとの比較において

本研究は,手軽で低温やけどの危険性がない遠赤外線素材のソックスの保温効果を明らかにし,臨床での活用の有無の検討の基礎資料とすることを目的とした。対象は,健康な成人女性32名で,ポリエステル素材のソックスおよびポリエステル素材にセラミックスを練り込ませた遠赤外線素材のソックスの着用の前・後の足底部の皮膚温度をサーモグラフィーで測定した。分析は,サーモグラフィーで記録された右足足底部の4点とし,それぞれの平均値の差をみた。以下の結果が得られた。1.ソックス着用により,足底部の皮膚温度は上昇する。2.ポリエステル素材ソックスと遠赤外線素材ソックス30分間着用の比較では,指先において温度上昇の有意な差をみた。The purpose of this study is to explain the effect of far infrared radiation materials easily and without low temperature burn risk. We investigated the possibility of using this socks for clinical use. Tested group were consisted of normal controled 32 adult women. We measured the temperature of the planter by the thermography. The temperature between before and after the using testing socks were compared. The temperature at the four points on the surface of the planter were serected for the comparison. Average value of the deference between before and after using these socks are tested by t-test. The results are 1. The temperrature of planter increased by the using both socks, 2. The increase of the temperature at the toe by the using after thirty minutes, far infrared radiation socks was higher than by the using polyesters socks

Optimization of pH Elution Conditions in Immune Complexome Analysis for Comprehensive Identification of Immune Complex Antigens

The identification of antigens incorporated into immune complexes (IC-antigens) is important for studying the pathophysiology of immunological diseases. Immune complexome analysis identifies IC-antigens by analyzing ICs collected from biological fluids by IC-capturing beads. In this study, we optimized the method to improve its comprehensiveness while maintaining selectivity for IC-antigens by comparing the number of identified peptides (model IC experiment) or proteins (human pooled serum) eluted from Protein G beads using different pH solutions (pH 2.0 - 11.0)

Expression, purification and characterization of the recombinant cysteine-rich antimicrobial peptide snakin-1 in Pichia pastoris

Snakin-1 (SN-1) is a small cysteine-rich plant antimicrobial peptide with broad spectrum antimicrobial activity which was isolated from potato (Solanum tuberosum). Here, we carried out the expression of a recombinant SN-1 in the methylotrophic yeast Pichia pastoris, along with its purification and characterization. A DNA fragment encoding the mature SN-1 was cloned into pPIC9 vector and introduced into P. pastoris. A large amount of pure recombinant SN-1 (approximately 40 mg/1L culture) was obtained from a fed-batch fermentation culture after purification with a cation exchange column followed by RP-HPLC. The identity of the recombinant SN-1 was verified by MALDI-TOF MS, CD and H-1 NMR experiments. All these data strongly indicated that the recombinant SN-1 peptide had a folding with six disulfide bonds that was identical to the native SN-1. Our findings showed that SN-1 exhibited strong antimicrobial activity against test microorganisms and produced very weak hemolysis of mammalian erythrocytes. The mechanism of its antimicrobial action against Escherichia coli was investigated by both outer membrane permeability assay and cytoplasmic membrane depolarization assay. These assays demonstrated that SN-1 is a membrane-active antimicrobial peptide which can disrupt both outer and cytoplasmic membrane integrity. This is the first report on the recombinant expression and purification of a fully active SN-1 in P. pastoris. (C) 2016 Elsevier Inc. All rights reserved

Exploring the In situ pairing of human galectins toward synthetic O-mannosylated core M1 glycopeptides of α-dystroglycan

Dystroglycan (DG), which constitutes a part of the dystrophin-glycoprotein complex, connects the extracellular matrix to the cytoskeleton. The matriglycans presented by the extracellular alpha-DG serve as a contact point with extracellular matrix proteins (ECM) containing laminin G-like domains, providing cellular stability. However, it remains unknown whether core M1 (GlcNAc beta 1-2Man) structures can serve as ligands among the various O-Mannosylated glycans. Therefore, based on the presence of N-acetylLactosamine (LacNAc) in this glycan following the core extension, the binding interactions with adhesion/growth-regulatory galectins were explored. To elucidate this process, the interaction between galectin (Gal)-1, -3, -4 and -9 with alpha-DG fragment (372)TRGAIIQTPTLGPIQPTRV(390) core M1-based glycopeptide library were profiled, using glycan microarray and nuclear magnetic resonance studies. The binding of galectins was revealed irrespective of its modular architecture, adding galectins to the list of possible binding partners of alpha-DG core M1 glycoconjugates by cis-binding (via peptide- and carbohydrate-protein interactions), which can be abrogated by alpha 2,3-sialylation of the LacNAc units. The LacNAc-terminated alpha-DG glycopeptide interact simultaneously with both the S- and F-faces of Gal-1, thereby inducing oligomerization. Furthermore, Gal-1 can trans-bridge alpha-DG core M1 structures and laminins, which proposed a possible mechanism by which Gal-1 ameliorates muscular dystrophies; however, this proposal warrants further investigation