Neutrino emission from Blazars.

During the last decade, multi-messenger astronomy has become increasingly relevant for the astrophysical community. In this context the discovery of PeV neutrinos by IceCube in 2012, in clear excess to the expected atmospheric flux at very-high energy ( 73 100 TeV ), marked the beginning of the high-energy neutrino astrophysics era. Differently from photons, neutrinos can carry information about the core of the astrophysical objects that produce them, giving us a better understanding of the internal composition of their sources. The origin of these neutrinos is still an open issue. Among the possible extragalactic neutrino sources, Blazars start to stand out. Blazars are associated to active galactic nuclei hosting a relativistic jet oriented close to our line of sight. The presence of jets and the strong non-thermal emission up to the TeV band makes them natural accelerators of particles. In September 2017 the spatial coincidence between a neutrino event detected by IceCube, with a good angular resolution, and a blazar, TXS 0506+056, was observed for the rst time. This event is even more intriguing because of the high-state emission in \u3b3-ray band of this blazar. During the three years of my PhD, I focused my attention on the study of blazar objects as neutrino emitters. In this thesis all my work on this topic is presented

Neutrino emission from Blazars.

During the last decade, multi-messenger astronomy has become increasingly relevant for the astrophysical community. In this context the discovery of PeV neutrinos by IceCube in 2012, in clear excess to the expected atmospheric flux at very-high energy (≳ 100 TeV ), marked the beginning of the high-energy neutrino astrophysics era. Differently from photons, neutrinos can carry information about the core of the astrophysical objects that produce them, giving us a better understanding of the internal composition of their sources. The origin of these neutrinos is still an open issue. Among the possible extragalactic neutrino sources, Blazars start to stand out. Blazars are associated to active galactic nuclei hosting a relativistic jet oriented close to our line of sight. The presence of jets and the strong non-thermal emission up to the TeV band makes them natural accelerators of particles. In September 2017 the spatial coincidence between a neutrino event detected by IceCube, with a good angular resolution, and a blazar, TXS 0506+056, was observed for the rst time. This event is even more intriguing because of the high-state emission in γ-ray band of this blazar. During the three years of my PhD, I focused my attention on the study of blazar objects as neutrino emitters. In this thesis all my work on this topic is presented

Greenhouse gas emissions and energy balance of biodiesel production from microalgae cultivated in photobioreactors in Denmark: a life-cycle modeling

The current use of fossil fuels is problematic for both environmental and economic reasons and biofuels are regarded as a potential solution to current energy issues. This study analyzes the energy balances and greenhouse gas emissions of 24 different technology scenarios for the production of algal biodiesel from Nannochloropsis cultivated at industrial scale in photobioreactors in Denmark. Both consolidated and pioneering technologies are analyzed focusing on strengths and weaknesses which influence the performance. Based on literature data, energy balance and greenhouse gas emissions are determined in a comparative 'well-to-tank' Life Cycle Assessment against fossil diesel. Use of by-products from biodiesel production such as glycerol obtained from transesterification and anaerobic digestion of residual biomass are included. Different technologies and methods are considered in cultivation stage (freshwater vs. wastewater; synthetic CO2 vs. waste CO2), harvesting stage (flocculation vs. centrifugation) and oil extraction stage (hexane extraction vs. supercritical CO2 extraction). The choices affecting environmental performance of the scenarios are evaluated. Results show that algal biodiesel produced through current conventional technologies has higher energy demand and greenhouse gas emissions than fossil diesel. However, greenhouse gas emissions of algal biodiesel can be significantly reduced through the use of 'waste' flows (nutrients and CO2) but there are still technical difficulties with both microalgae cultivation in wastewater as well as transportation and injection of waste CO2. In any way, a positive energy balance is still far from being achieved. Considerable improvements must be made to develop an environmentally beneficial microalgae biodiesel production on an industrial scale. In particular, different aspects of cultivation need to be enhanced, such as the use of wastewater and CO2-rich flue gas from industrial power plants

Role of Cinchona Alkaloids in the Enantio- and Diastereoselective Synthesis of Axially Chiral Compounds

Asymmetric synthesis using organic catalysts has evolved since it was first realized and defined. Nowadays, it can be considered a valid alternative to transition metal catalysis for synthesizing chiral molecules. According to the literature, the number of asymmetric organocatalytic processes associated with atropisomer synthesis has rapidly increased over the past 10 years because organocatalysis addresses the challenges posed by the most widespread strategies used for preparing axially chiral molecules with satisfactory results. These strategies, useful to prepare a wide range of C–C, C–heteroatom, and N–N atropisomers, vary from kinetic resolution to direct arylation, desymmetrization, and central-to-axial chirality conversion. In this field, our contribution focuses on determining novel methods for synthesizing atropisomers, during which, in most cases, the construction of one or more stereogenic centers other than the stereogenic axis occurred. To efficiently address this challenge, we exploited the ability of catalysts based on a cinchona alkaloid scaffold to realize enantioselective organic transformations. Desymmetrization of N-(2-tert-butylphenyl) maleimides was one of the first strategies that we pursued for preparing C–N atropisomers. The main principle is based on the presence of a rotationally hindered C–N single bond owing to the presence of a large tert-butyl group. Following the peculiar reactivity of this type of substrate as a powerful electrophile and dienophile, we realized several transformations. First, we investigated the vinylogous Michael addition of 3-substituted cyclohexenones, where a stereogenic axis and two contiguous stereocenters were concomitantly and remotely formed and stereocontrolled using a primary amine catalyst. Subsequently, we realized desymmetrization via an organocatalytic Diels–Alder reaction of activated unsaturated ketones that enabled highly atropselective transformation with efficient diastereoselectivity, thereby simultaneously controlling four stereogenic elements. Employing chiral organic bases allowed us to realize efficient desymmetrizations using carbon nucleophiles, such as 1,3-dicarbonyl compounds, cyanoacetates, and oxindoles. These reactions, performed with different types of catalysts, highlighted the versatility of organocatalysis as a powerful strategy for atropselective desymmetrization of pro-axially chiral maleimides. Hereafter, we studied the Friedel–Crafts alkylation of naphthols with indenones, a powerful method for enantioselective synthesis of conformationally restricted diastereoisomeric indanones. We realized the first axially chiral selective Knoevenagel condensation using cinchona alkaloid primary amine as the catalyst. This reaction provided a powerful method to access enantioenriched olefins containing the oxindole core. Subsequently, we initiated an intense program for the computational investigation of the reaction mechanism of our atropselective processes. An understanding of the catalytic activity for vinylogous atropselective desymmetrization as well as of the role played by the acidic cocatalyst used for the experimental work was achieved. Recently, we have garnered interest in the novel frontiers of atropselective synthesis. As observed in recent publications, there is considerable interest in the development of methods for preparing N–N atropisomers, an emerging topic in the field of atropselective synthesis. We focused on the synthesis of hydrazide atropisomers by developing a one-pot sequential catalysis protocol based on two sequential organocatalytic reactions that provided high stereocontrol of two contiguous stereogenic elements

Astro-photography as an effective tool for Outreach and Education: IACT in exposition

In our epoch, images are a powerful way to convey a message to a large audience. Through the use of amazing astronomical photographs, science can be communicated effectively at different levels, to a very diverse audience of all ages. In fact, astrophotography combines aesthetic appeal with the illustration of the science behind astronomical phenomena. This is the aim of the exhibit "A che Punto \`e la NOTTE - A scientific exhibition of astrophotography" organized by us in Italy, in October 2020, with the partnership of the cultural association PhysicalPub. Many different authors, both single individuals and professional or amateur observatories, were asked to send their best pictures. The 54 astronomical images chosen by a scientific committee, categorised in three different topics (night landscape, deep sky, instrumentation), were seen by more than 2000 visitors and 11 school groups (despite the difficult period due to the COVID pandemic). A free audio-guide, available on-line through a web-application developed on purpose, delivered scientific explanations of images for self-guided tours. Conferences and guided tours were also organized. The highlight of the exhibit were four mirrors from the MAGIC telescope and an ASTRI scale-model that allowed an in-depth description of how an Imaging Atmospheric Cherenkov Telescope (IACT) works, introducing the science of VHE cosmic radiation. We will summarize the main difficulties in organizing this event and the feedback we had from the visitors. The exhibit is still available online, visiting the website mostrascientifica.it or via the web audio-guide (english and italian) at guida.mostrascientifica.it.Comment: 8 pages, 5 figures, Proceedings of the 37th International Cosmic Ray Conference (ICRC 2021), Berlin, German

On the detectability of BL Lac objects by IceCube

Since 2010 IceCube observed around 50 high-energy neutrino events of cosmic origin above 60 TeV, but the astrophysical sources of these events are still unknown. We recently proposed high-energy emitting BL Lac (HBL) objects as candidate emitters of high-energy neutrinos. Assuming a direct proportionality between high-energy gamma-ray and very-high energy neutrino fluxes, we calculated the expected neutrino event number in a year for IceCube and the presently under construction Km3NeT. To give a value of the significance of a detection we considered also the background for the single sources. To this aim we derived the through-going muon rate, generated by muon neutrino including the effect of Earth absorption, the density of the Earth and the cross section $\nu N$. Applying this calculation both to HBL sources and the atmospherical neutrino background, we can calculate the expected significance of the detection by IceCube, showing that our scenario is compatible with a no detection of HBL

Assessing the hydrolytic fate of the masked mycotoxin zearalenone-14-glucoside–A warning light for the need to look at the “maskedome”

Masked mycotoxins are plant metabolites of mycotoxins that contaminate food and feed. They pose health concern as the shortage of toxicological data forces the lack of regulation worldwide. The present work investigated the toxicological relevance of the masked mycotoxin zearalenone-14-glucoside. In vitro, it shows a lower toxicity in respect to the parent compound. However, the major risks related to the consumption of masked mycotoxins depend on the possibility to undergo hydrolysis. Therefore, the hydrolysis and further transformation of zearalenone-14-glucoside in bovine blood and blood components (i.e. plasma, serum and serum albumin) were monitored using LC/MS-MS analysis to gain insights on the possible systemic fate. Hydrolysis was observed in all matrices, and both cell-dependent and eindependent contributions were pointed out. Moreover, further metabolism was observed in the whole blood as zearalenol isomers were found. Serum albumin was identified among the active components, and the protein-ligand interaction was investigated via computational analysis. The blood has been pointed out as possible district of reversion and further activation of zearalenone-14-glucoside, and a similar fate cannot be excluded for other masked mycotoxins. Therefore, the systemic hydrolysis should be evaluated beside the absorption, bioavailability and bioaccessibility to deeply understand the toxicity of masked mycotoxins

Precocious Puberty and Covid-19 Into Perspective: Potential Increased Frequency, Possible Causes, and a Potential Emergency to Be Addressed

A significant increase in precocious puberty, rapidly progressive puberty and precocious menarche has been reported in Italy since the initial lockdown because of the pandemic, and this could represent a new emergency to be addressed during this pandemic. There is a need, therefore, for further understanding and research. Many causes could account for this. Initially, it was thought that the changes in life-style, in screen time, and sleeping habits could be the cause but if considered individually these are insufficient to explain this phenomenon. Likely, changes in central nervous mediators, and an increase in catecholamines could contribute as a trigger, however, these aspects are poorly studied and understood as well as the real perceptions of these children. Finally, staying more indoors has certainly exposed these children to specific contaminants working as endocrine disruptors which could also have had an effect. It would be of utmost importance to compare this phenomenon worldwide with appropriate studies in order to verify what is happening, and gain a new insight into the consequences of the covid-19 pandemic and into precocious puberty and for future prevention

Life cycle assessment and energy balance of a novel polyhydroxyalkanoates production process with mixed microbial cultures fed on pyrolytic products of wastewater treatment sludge

A "cradle-to-grave" life cycle assessment is performed to identify the environmental issues of polyhydroxyalkanoates (PHAs) produced through a hybrid thermochemical-biological process using anaerobically digested sewage sludge (ADSS) as feedstock. The assessment includes a measure of the energy performance of the process. The system boundary includes: (i) Sludge pyrolysis followed by volatile fatty acids (VFAs) production; (ii) PHAs-enriched biomass production using a mixed microbial culture (MMC); (iii) PHAs extraction with dimethyl carbonate; and iv) PHAs end-of-life. Three scenarios differing in the use of the syngas produced by both pyrolysis and biochar gasification, and two more scenarios differing only in the external energy sources were evaluated. Results show a trade-off between environmental impacts at global scale, such as climate change and resources depletion, and those having an effect at the local/regional scale, such as acidification, eutrophication, and toxicity. Process configurations based only on the sludge-to-PHAs route require an external energy supply, which determines the highest impacts with respect to climate change, resources depletion, and water depletion. On the contrary, process configurations also integrating the sludge-to-energy route for self-sustainment imply more onsite sludge processing and combustion; this results in the highest values of eutrophication, ecotoxicity, and human toxicity. There is not a categorical winner among the investigated configurations; however, the use of a selected mix of external renewable sources while using sludge to produce PHAs only seems the best compromise. The results are comparable to those of both other PHAs production processes found in the literature and various fossil-based and bio-based polymers, in terms of both non-biogenic GHG emissions and energy demand. Further process advancements and technology improvement in high impact stages are required to make this PHAs production process a competitive candidate for the production of biopolymers on a wide scale

The Fermi blazar sequence

We revisit the blazar sequence exploiting the complete, flux-limited sample of blazars with known redshift detected by the Fermi satellite after 4 yr of operations (the 3LAC sample). We divide the sources into γ-ray luminosity bins, collect all the archival data for all blazars, and construct their spectral energy distribution (SED). We describe the average SED of blazars in the same luminosity bin through a simple phenomenological function consisting of two broken power laws connecting with a power law describing the radio emission. We do that separately for BL Lacs and for flat spectrum radio quasars (FSRQs) and also for all blazars together. The main results are: (I) FSRQs display approximately the same SED as the luminosity increases, but the relative importance of the high-energy peak increases; (II) as a consequence, the X-ray spectra of FSRQs become harder for larger luminosities; (III) BL Lacs indeed form a sequence: they become redder (I.e. smaller peak frequencies) with increasing luminosities, with a softer γ-ray slope and a larger dominance of the high-energy peak; (IV) for all blazars (BL Lacs+FSRQs), these properties become more prominent, as the highest luminosity bin is populated mostly by FSRQs and the lowest luminosity bin mostly by BL Lacs. This agrees with the original blazar sequence, although BL Lacs never have an average γ-ray slope as hard as found in the original sequence. (v) At high luminosities, a large fraction of FSRQs show signs of thermal emission from the accretion disc, contributing to the optical-UV (ultraviolet)