A fluid flow perspective on the diagenesis of Te Aute limestones

Pliocene cool-water, bioclastic Te Aute limestones in East Coast Basin, New Zealand, accumulated either in shelfal shoal areas or about structurally shallow growth fold structures in the tectonically active accretionary forearc prism. Up to five stages of carbonate cementation are recognised, based on cement sequence-stratigraphic concepts, that formed on the seafloor during exposure of the limestones before burial, during burial, uplift, and deformation. Two principal fluid types are identified--topography-driven meteoric fluids and compaction-driven fluids. We have developed conceptual and quantitative models that attempt to relate the physical characteristics of fluid flow to the cement paragenesis. In particular, we have simulated the effects of uplift of the axial ranges bordering East Coast Basin in terms of the degree of penetration of a meteoric wedge into the basin. The dynamics of meteoric flow changed dramatically during uplift over the last 2 m.y. such that the modelled extent of the meteoric wedge is at least 40 km across the basin, and the penetration depth 1500 m or more corresponding with measured freshwater intersections in some oil wells. Cement-fluid relationships include: (1) true marine cements that precipitated in areas remote from shallow freshwater lenses; (2) pre-compaction cements that formed in shallow freshwater lenses beneath limestone "islands"; (3) post-compaction cements derived from compaction-driven flow during burial; (4) early uplift-related fracture-fill cements formed during deformation of the accretionary prism and uplift of the axial ranges; and (5) late uplift-related cements associated with uplift into a shallow meteoric regime

Canadian Arctic–Beaufort Sea Rifted Margin Tectono-Sedimentary Element, SE Canada Basin

Peer reviewedPostprin

Note on paramoudra-like carbonate concretions in the Urenui Formation, North Taranaki: possible plumbing system for a Late Miocene methane seep field

A reconnaissance study of calcitic and dolomitic tubular concretions in upper slope mudstone of the Late Miocene Urenui Formation exposed along the north Taranaki coastline indicates that they have a complex diagenetic history involving different phases of carbonate cementation and likely hydrofracturing associated with build up of fluid/gas pressures. The concretions resemble classical paramoudra in the European chalk, but are not siliceous and do not have a trace fossil origin. Stable oxygen and carbon isotope data suggest that the micritic carbonate cements in the Urenui paramoudra were probably sourced primarily from ascending methane fluid/gases, and that they precipitated entirely within the host mudstone below the seafloor. We suggest the paramoudra may mark the subsurface plumbing networks of a Late Miocene cold seep system, in which case they have relevance to the evolution and migration of hydrocarbons in Taranaki Basin, at this site perhaps focussed along the Taranaki Fault. The presence of dislodged and mass-emplaced paramoudra in the axial conglomerate of channels within the Urenui mudstone suggests there could be a connection between the loci of seep field development and slope failure and canyon cutting on the Late Miocene Taranaki margin

An Assessment of the Needs of Georgian Secondary Agricultural Educators

This purpose of this study was to determine the perception of secondary educators involved in Future Farmers of Georgia (FFG) schools throughout the country of Georgia concerning their needs for furthering the existing framework of agricultural education. The researchers used a modified focus group method to meet the aformentioned purpose of this study. The population for this study consisted of the high school agricultural education teachers and their administrators who were participating in the FFG Program (n=14). Qualitative data collected from the probe (first round) was used to generate a series of 46 statements. In the second round participants were asked to rank the 46 statements using a five point Likert-type scale. Due to events in the Former Republic of Georgia, the third round could not be conducted. Therefore, the researchers concluded that the round two data would be used to meet the purpose and objectives of this study. Participants strongly agreed that the FFG program strengthens leadership skills among their students and students have become more involved in practical/labor activities. They also agreed that gaining knowledge and skills from the FFG program will improve the future development of agricultural education in the country. Participants also strongly agreed that improvement of schools’ materials, technology, and development are necessary to improve the FFG program. Participants were either neutral or agreed with the following statements: due to the FFG program, academic performance has been increased among students; and students have greater academic success in history and geography

Can Text Messages Reach the Parts Other Process Measures Cannot Reach: An Evaluation of a Behavior Change Intervention Delivered by Mobile Phone?

BackgroundProcess evaluation is essential in developing, piloting and evaluating complex interventions. This often involves observation of intervention delivery and interviews with study participants. Mobile telephone interventions involve no face to face contact, making conventional process evaluation difficult. This study assesses the utility of novel techniques for process evaluation involving no face to face contact.MethodsText messages were delivered to 34 disadvantaged men as part of a feasibility study of a brief alcohol intervention. Process evaluation focused on delivery of the text messages and responses received from study participants. The computerized delivery system captured data on receipt of the messages. The text messages, delivered over 28 days, included nine which asked questions. Responses to these questions served as one technique for process evaluation by ascertaining the nature of engagement with the study and with steps on the causal chain to behavior change.ResultsA total of 646 SMS text messages were sent to participants. Of these, 613 messages (95%) were recorded as delivered to participants’ telephones. 88% of participants responded to messages that asked questions. There was little attenuation in responses to the questions across the intervention period. Content analysis of the responses revealed that participants engaged with text messages, thought deeply about their content and provided carefully considered personal responses to the questions.ConclusionsSocially disadvantaged men, a hard to reach population, engaged in a meaningful way over a sustained period with an interactive intervention delivered by text message. The novel process measures used in the study are unobtrusive, low cost and collect real-time data on all participants. They assessed the fidelity of delivery of the intervention and monitored retention in the study. They measured levels of engagement and identified participants’ reactions to components of the intervention. These methods provide a valuable addition to conventional process evaluation techniques

Identification and characterization of an affimer affinity reagent for the detection of the cAMP sensor, EPAC1

An exchange protein directly activated by cAMP 1 (EPAC1) is an intracellular sensor for cAMP that is involved in a wide variety of cellular and physiological processes in health and disease. However, reagents are lacking to study its association with intracellular cAMP nanodomains. Here, we use non-antibody Affimer protein scaffolds to develop isoform-selective protein binders of EPAC1. Phage-display screens were carried out against purified, biotinylated human recombinant EPAC1ΔDEP protein (amino acids 149–811), which identified five potential EPAC1-selective Affimer binders. Dot blots and indirect ELISA assays were next used to identify Affimer 780A as the top EPAC1 binder. Mutagenesis studies further revealed a potential interaction site for 780A within the EPAC1 cyclic nucleotide binding domain (CNBD). In addition, 780A was shown to co-precipitate EPAC1 from transfected cells and co-localize with both wild-type EPAC1 and a mis-targeting mutant of EPAC1(K212R), predominantly in perinuclear and cytosolic regions of cells, respectively. As a novel EPAC1-selective binder, 780A therefore has the potential to be used in future studies to further understand compartmentalization of the cAMP-EPAC1 signaling system

Modification of EGF-Like Module 1 of Thrombospondin-1, an Animal Extracellular Protein, by O-Linked N-Acetylglucosamine

Thrombospondin-1 (TSP-1) is known to be subject to three unusual carbohydrate modifications: C-mannosylation, O-fucosylation, and O-glucosylation. We now describe a fourth: O-β-N-acetylglucosaminylation. Previously, O-β-N-acetylglucosamine (O-β-GlcNAc) was found on a threonine in the loop between the fifth and sixth cysteines of the 20th epidermal growth factor (EGF)-like module of Drosophila Notch. A BLAST search based on the Drosophila Notch loop sequence identified a number of human EGF-like modules that contain a similar sequence, including EGF-like module 1 of TSP-1 and its homolog, TSP-2. TSP-1, which has a potentially modifiable serine in the loop, reacted in immuno-blots with the CTD110.6 anti-O-GlcNAc antibody. Antibody reactivity was diminished by treatment of TSP-1 with β-N-acetylhexosaminidase. TSP-2, which lacks a potentially modifiable serine/threonine in the loop, did not react with CTD110.6. Analysis of tandem modules of TSP-1 localized reactivity of CTD110.6 to EGF-like module 1. Top-down mass spectrometric analysis of EGF-like module 1 demonstrated the expected modifications with glucose (+162 Da) and xylose (+132 Da) separately from modification with N-acetyl hexosamine (+203 Da). Mass spectrometric sequence analysis localized the +203-Da modification to Ser580 in the sequence 575CPPGYSGNGIQC586. These results demonstrate that O-β-N-acetylglucosaminylation can occur on secreted extracellular matrix proteins as well as on cell surface proteins