A Comparative Study of Aldehyde Oxidase from Tumorous-Head and Oregon-R-C Strains of Drosophila Melanogaster

Aldehyde oxidase has been partially purified from Oregon-R-C and tuh(ASU) strains of Drosophila melanogaster using an affinity technique. The two enzymes were subjected to a partial kinetic analysis and found to be very similar to one another. This indicated the problem of elevated aldehyde oxidase activity in tuh(ASU) at key developmental stages (Kuhn and Cunningham, 1976) is due to an abnormal regulation. A comparative isozyme study through the developmental stages showed no major differences between the enzymes indirectly supporting the idea of an abnormal regulation. A comparison of tuh(ASU) with four wild-type strains indicated it may be a fourth allozyme of aldehyde oxidase

Intensive Survey of San Gabriel River Segment 1248: August 20-24, 1985

Survey report documenting the water of the San Gabriel River between August 20 to August 24 198

Intensive Survey of Blanco River Segment 1809: June 5-7, 1985

Survey report documenting the findings in the waters of Blanco River from June 3 to June 7, 1985

HIV-1 viral load assays for resource-limited settings: Authors' reply [5]

The authors discuss studies on the low-cost viral load assays that are currently available and their potential for use in resource-limited settings

HIV-1 Viral loas assays for resource-limited settings

Tremendous strides have been made in treating HIV-1 infection in industrialized countries. Combination therapy with antiretroviral (ARV) drugs suppresses virus replication, delays disease progression, and reduces mortality. In industrialized settings, plasma viral load assays are used in combination with CD4 cell counts to determine when to initiate therapy and when a regimen is failing. In addition, unlike serologic assays, these assays may be used to diagnose perinatal or acute HIV-1 infection. Unfortunately, the full benefits of antiretroviral drugs and monitoring tests have not yet reached the majority of HIV-1-infected patients who live in countries with limited resources. In this article we discuss existing data on the performance of alternative viral load assays that might be useful in resource-limited settings

Intensive Survey of Sabine River Segment 0505: September 24-28, 1984

Survey report documenting the findings in the waters of Sabine River from September 24 to September 28, 1984

Seasonal variations in selected physical and chemical conditions of a small impoundment in Brazos County, Texas

Due to the character of the original source materials and the nature of batch digitization, quality control issues may be present in this document. Please report any quality issues you encounter to [email protected], referencing the URI of the item.Not availabl

Intensive Survey of Salado Creek Segment 1243: October 7-10, 1985; July 15-18, 1986

Survey report documenting the water of Salado Creek in October 7 to October 10 1985 and July 15 to July 18 198

Ultrasensitive p24 Antigen Assay for Diagnosis of Perinatal Human Immunodeficiency Virus Type 1 Infection▿

We evaluated an ultrasensitive p24 antigen enzyme immunosorbent assay on 802 plasma specimens from 582 infants and children of 0 to 180 days of age. Overall sensitivity and specificity were 91.7% and 98.5%, respectively. After exclusion of infants of less than 7 days of age, the sensitivity and specificity were 93.7% and 98.3%, respectively

Comparative Analysis of Two Commercial Phenotypic Assays for Drug Susceptibility Testing of Human Immunodeficiency Virus Type 1

Human immunodeficiency virus type 1 (HIV-1) isolates from 50 plasma specimens were analyzed for phenotypic susceptibility to licensed reverse transcriptase inhibitors and protease inhibitors by the Antivirogram and PhenoSense HIV assays. Twenty of these specimens were from recently seroconverted drug-naïve persons, and 30 were from patients who were the sources of occupational exposures to HIV-1; 16 of the specimens in the latter group were from drug-experienced patients. The phenotypic results of the Antivirogram and PhenoSense HIV assays were categorized as sensitive or reduced susceptibility on the basis of the cutoff values established by the manufacturers of each assay. Data for 12 to 15 drugs were available by both assays for 38 specimens and represented a total of 529 pairs of results. The two data sets had a 91.5% concordance by phenotypic category. The discordant results (n = 45) were distributed randomly among 26 specimens and included 28 results (62.2%) which were within a twofold difference of the assay cutoff values. None of the discordant results were associated with primary resistance mutations that predicted high-level (>20-fold) resistance. Discordant results were distributed equally among specimens from drug-experienced and drug-naïve individuals and were slightly higher for protease inhibitors than for nonnucleoside reverse transcriptase inhibitors or nucleoside reverse transcriptase inhibitors. The findings of the present study demonstrate that the results of the Antivirogram and PhenoSense HIV assays correlate well, despite the use of different testing strategies