Cdc42 Effector Protein 2 (XCEP2) is required for normal gastrulation and contributes to cellular adhesion in Xenopus laevis

BACKGROUND: Rho GTPases and their downstream effector proteins regulate a diverse array of cellular processes during embryonic development, including reorganization of cytoskeletal architecture, cell adhesion, and transcription. Changes in the activation state of Rho GTPases are converted into changes in cellular behavior by a diversity of effector proteins, which are activated in response to changes in the GTP binding state of Rho GTPases. In this study we characterize the expression and function of one such effector, XCEP2, that is present during gastrulation stages in Xenopus laevis. RESULTS: In a search for genes whose expression is regulated during early stages of embryonic development in Xenopus laevis, a gene encoding a Rho GTPase effector protein (Xenopus Cdc42 effector protein 2, or XCEP2) was isolated, and found to be highly homologous, but not identical, to a Xenopus sequence previously submitted to the Genbank database. These two gene sequences are likely pseudoalleles. XCEP2 mRNA is expressed at constant levels until mid- to late- gastrula stages, and then strongly down-regulated at late gastrula/early neurula stages. Injection of antisense morpholino oligonucleotides directed at one or both pseudoalleles resulted in a significant delay in blastopore closure and interfered with normal embryonic elongation, suggesting a role for XCEP2 in regulating gastrulation movements. The morpholino antisense effect could be rescued by co-injection with a morpholino-insensitive version of the XCEP2 mRNA. Antisense morpholino oligonucleotides were found to have no effect on mesodermal induction, suggesting that the observed effects were due to changes in the behavior of involuting cells, rather than alterations in their identity. XCEP2 antisense morpholino oligonucleotides were also observed to cause complete disaggregation of cells composing animal cap explants, suggesting a specific role of XCEP2 in maintenance or regulation of cell-cell adhesion in early embryos. This loss of cell adhesion could be rescued by co-injection with a morpholino-insensitive version of the XCEP2 mRNA. CONCLUSIONS: XCEP2 appears to be an essential component in the early developmental program in Xenopus laevis. XCEP2 is involved in maintenance of cell-cell adhesion, and as such may constitute a regulatory component that could help to balance the need for tissue integrity and plasticity during the dynamic cellular rearrangements of gastrulation

Appropriating the Returns from Industrial Research and Development

macroeconomics, industrial research and development, patent law

Risk reduction methodologies and technologies for the Earth Observing System (EOS) Operations Center (EOC)

This paper will discuss proposed Flight Operations methodologies and technologies for the Earth Observing System (EOS) Operations Center (EOC), to reduce risks associated with the operation of complex multi-instrument spacecraft in a multi-spacecraft environment. The EOC goals are to obtain 100 percent science data capture and maintain 100 percent spacecraft health, for each EOS spacecraft. Operations risks to the spacecraft and data loss due to operator command error, mission degradation due to mis-identification of an anomalous trend in component performance or mis-management of resources, and total mission loss due to improper subsystem configuration or mis-identification of an anomalous condition. This paper discusses automation of routine Flight Operations Team (FOT) responsibilities, Expert systems for real-time non-nominal condition decision support, and Telemetry analysis systems for in-depth playback data analysis and trending

On the Sources and Significance of Interindustry Differences in Technological Opportunities

The set of technological opportunities in a given industry is one of the fundamental determinants of technical advance in that line of business. We examine the concept of technological opportunity and discuss three categories of sources of those opportunities: advances in scientific understanding and technique, technological advances originating in other industries and in other private and governmental institutions, and feedbacks from an industry’s own technological advances. Data from the Yale Survey on Industrial Research and Development are used to measure the strength of various sources of technological opportunity and to discern interindustry differences in the importance of these sources. We find that interindustry differences in the strength and sources of technological opportunities contribute importantly to explanations of cross-industry variation in R&D intensity and technological advance

Appropriating the Returns from Industrial R&D

In this paper, we describe the results of an inquiry into the nature of appropriability conditions in over one hundred manufacturing industries, and we discuss how this information has been and might be used to cast light on important issues in the economics of innovation and public policy. Our data, derived from a survey of high-level R&D executives, are informed opinions about the nature of an industry’s technological and economic environment rather than quantitative measures of inputs and outputs

The full genome sequence of three strains of Jamestown Canyon virus and their pathogenesis in mice or monkeys

<p>Abstract</p> <p>Background</p> <p>Jamestown Canyon virus (JCV), family <it>Bunyaviridae</it>, is a mosquito-borne pathogen endemic in the United States and Canada that can cause encephalitis in humans and is considered an emerging threat to public health. The virus is genetically similar to Inkoo virus circulating in Europe, suggesting that much of the northern hemisphere contains JCV or similar variants.</p> <p>Results</p> <p>We have completed the sequence of three isolates of JCV collected in geographically diverse locations over a 57 year time span. The nucleotide identity for the three strains is 90, 83, and 85% for the S, M, and L segments respectively whereas the percent identify for the predicted amino acid sequences of the N, NS_S, M poly, G_N, NS_M, G_C, and L proteins was 97, 91, 94, 98, 91, 94, and 97%, respectively. In Swiss Webster mice, each JCV isolate exhibits low neuroinvasiveness but high infectivity. Two of the three JCV isolates were highly neurovirulent after IC inoculation whereas one isolate, JCV/03/CT, exhibited low neurovirulence. In rhesus monkeys, JCV infection is accompanied by a low-titered viremia, lack of clinical disease, but a robust neutralizing antibody response.</p> <p>Conclusions</p> <p>The first complete sequence of JCV is reported for three separate isolates, and a relatively high level of amino acid sequence conservation was observed even for viruses isolated 57 years apart indicating that the virus is in relative evolutionary stasis. JCV is highly infectious for mice and monkeys, and these animals, especially mice, represent useful experimental hosts for further study.</p

Compassion, Dominance/Submission, and Curled Lips: A Thematic Analysis of Dacryphilic Experience

Paraphilias are often discussed in the psychological literature as pathological problems, yet relatively little research exists that looks into non-pathological paraphilias (i.e., non-normative sexual interests). Empirical evidence suggests that many individuals incorporate a range of non-normative sexual interests into their sexual lifestyles. Dacryphilia is a non-normative sexual interest that involves enjoyment or arousal from tears and crying, and to date has never been researched empirically. The present study set out to discover the different interests within dacryphilia and explore the range of dacryphilic experience. A set of online interviews was carried out with individuals with dacryphilic preferences and interests (six females and two males) from four countries. The data were analysed for semantic and latent themes using thematic analysis. The respondents' statements focused attention on three distinct areas that may be relevant to the experience of dacryphilia: (i) compassion; (ii) dominance/submission; and (iii) curled-lips. The data provided detailed descriptions of features within all three interests, which are discussed in relation to previous quantitative and qualitative research within emotional crying and tears, and the general area of non-normative sexual interests. The study suggests new directions for potential research both within dacryphilia and with regard to other non-normative sexual interests

The Formation of Fragments at Corotation in Isothermal Protoplanetary Disks

Numerical hydrodynamics simulations have established that disks which are evolved under the condition of local isothermality will fragment into small dense clumps due to gravitational instabilities when the Toomre stability parameter $Q$ is sufficiently low. Because fragmentation through disk instability has been suggested as a gas giant planet formation mechanism, it is important to understand the physics underlying this process as thoroughly as possible. In this paper, we offer analytic arguments for why, at low $Q$, fragments are most likely to form first at the corotation radii of growing spiral modes, and we support these arguments with results from 3D hydrodynamics simulations.Comment: 21 pages, 1 figur