Ecology of Testate Amoebae from Mires in the Central Rhodope Mountains, Greece and Development of a Transfer Function for Palaeohydrological Reconstruction

Testate amoebae are useful environmental indicators in ecological and palaeoecological studies from peatlands. Previous quantitative studies have focused on the peatlands of Northern and Central Europe, North America, and New Zealand and have considered a relatively restricted variety of peatland types, mostly ombrotrophic or Sphagnum-dominated while more minerotrophic fens have been less studied. Here we present the first quantitative ecological study of testate amoebae from four small mesotrophic fens (pH 5.5-8.1) in the Elatia Forest, northern Macedonia province, Greece. Relationships with the environmental data were investigated using redundancy analysis and mantel tests. Transfer function models were derived using a variety of techniques. Results demonstrate that as for Sphagnum-dominated mires hydrology is the most important control on amoebae community composition. Transfer function models should enable water tables to be predicted within 2.5 cm, when data selection is used this is reduced to less than 2 cm. pH is also an important environmental control on testate amoebae communities, a transfer function model enables pH prediction within 0.4 pH units. The hydrological transfer function is the best performing such model yet produced in terms of prediction error. This study provides new data on the ecology of testate amoebae in fens, and the transfer function models should allow quantitative palaeohydrological reconstruction

Extending the late holocene tephrochronology of the central Kenai Peninsula, Alaska

Tephrochronology, the reconstruction of past volcanic ash deposition, provides a valuable method for dating sediments and determining long-term volcanic history. Tephra layers are highly numerous in Alaska, but knowledge of their occurrence and distribution is incomplete. This study expands the regional tephrochronology for the Kenai Peninsula of southcentral Alaska by investigating the tephrostratigraphy of two peatland sites. We located seven visible tephras and seven microtephras and investigated the particle size and geochemistry of the visible tephras. Radiocarbon dates were used to estimate the timescale of each core. Geochemical comparison showed that the visible tephras originated from late Holocene eruptions of Augustine, Crater Peak-Mt. Spurr, and Hayes volcanoes. Some of the tephras had been documented previously, and these new findings expand their known range. Others represent eruptions not previously reported, including a Crater Peak-Mt. Spurr eruption around 430 cal. BP. The results provide new tephra data for the region, illustrate the spatial heterogeneity of tephra deposition, and show the potential of microtephras for expanding the regional tephra record

A power-saving modulation technique for time-of-flight range imaging sensors

Time-of-flight range imaging cameras measure distance and intensity simultaneously for every pixel in an image. With the continued advancement of the technology, a wide variety of new depth sensing applications are emerging; however a number of these potential applications have stringent electrical power constraints that are difficult to meet with the current state-of-the-art systems. Sensor gain modulation contributes a significant proportion of the total image sensor power consumption, and as higher spatial resolution range image sensors operating at higher modulation frequencies (to achieve better measurement precision) are developed, this proportion is likely to increase. The authors have developed a new sensor modulation technique using resonant circuit concepts that is more power efficient than the standard mode of operation. With a proof of principle system, a 93–96% reduction in modulation drive power was demonstrated across a range of modulation frequencies from 1–11 MHz. Finally, an evaluation of the range imaging performance revealed an improvement in measurement linearity in the resonant configuration due primarily to the more sinusoidal shape of the resonant electrical waveforms, while the average precision values were comparable between the standard and resonant operating modes

Heterodyne range imaging as an alternative to photogrammetry

Solid-state full-field range imaging technology, capable of determining the distance to objects in a scene simultaneously for every pixel in an image, has recently achieved sub-millimeter distance measurement precision. With this level of precision, it is becoming practical to use this technology for high precision three-dimensional metrology applications. Compared to photogrammetry, range imaging has the advantages of requiring only one viewing angle, a relatively short measurement time, and simplistic fast data processing. In this paper we fist review the range imaging technology, then describe an experiment comparing both photogrammetric and range imaging measurements of a calibration block with attached retro-reflective targets. The results show that the range imaging approach exhibits errors of approximately 0.5 mm in-plane and almost 5 mm out-of-plane; however, these errors appear to be mostly systematic. We then proceed to examine the physical nature and characteristics of the image ranging technology and discuss the possible causes of these systematic errors. Also discussed is the potential for further system characterization and calibration to compensate for the range determination and other errors, which could possibly lead to three-dimensional measurement precision approaching that of photogrammetry

The Waikato range imager

We are developing a high precision simultaneous full-field acquisition range imager. This device measures range with sub millimetre precision in range simultaneously over a full-field view of the scene. Laser diodes are used to illuminate the scene with amplitude modulation with a frequency of 10MHz up to 100 MHz. The received light is interrupted by a high speed shutter operating in a heterodyne configuration thus producing a low-frequency signal which is sampled with a digital camera. By detecting the phase of the signal at each pixel the range to the scene is determined. We show 3D reconstructions of some viewed objects to demonstrate the capabilities of the ranger

Can testate amoeba-based palaeohydrology be extended to fens?

Numerous palaeoecological studies have used testate amoeba analysis to reconstruct Holocene hydrological change in peatlands, and thereby past climatic change. Current studies have been almost exclusively restricted to ombrotrophic bogs and the period since the fen–bog transition. Although the critical link between peatland surface wetness and climate is less direct in minerotrophic peatlands, such records may still be of value where there are few others, particularly if multiple records can be derived and inter-compared. Expanding the temporal and spatial scope of testate amoeba-based palaeohydrology to minerotrophic peatlands requires studies to establish the primacy of hydrology and the efficacy of transfer functions across a range of sites. This study analyses testate amoeba data from wetlands spanning the trophic gradient in the eastern Mediterranean region. Results demonstrate that different types of wetlands have distinctly different amoeba communities, but hydrology remains the most important environmental control (despite water table depth being measured at different times for different sites). Interestingly, Zn and Fe emerge as significant environmental variables in a subset of sites with geochemical data. Testate amoeba–hydrology transfer functions perform well in cross-validation but frequently perform poorly when applied to other sites, particularly with sites of a different nutrient status. It may be valid to use testate amoebae to reconstruct hydrological change from minerotrophic peatlands with an applicable transfer function; however, it may not be appropriate to use testate amoebae to reconstruct hydrological change through periods of ecosystem evolution, particularly the fen–bog transition. In practice, the preservation of amoeba shells is likely to be a key problem for palaeoecological reconstruction from fens. Copyright © 2010 John Wiley & Sons, Ltd

Seven Reasons Why Protists Make Useful Bioindicators

To introduce the special issue this paper reviews the use of protists as bioindicators. Seven key advantages of protist bioindicators are highlighted, namely: environmental sensitivity, functional importance, distribution, size and numbers, response times, ease of analysis and preservation potential. Protist bioindicators have been used in a wide range of contexts from ecosystem restoration to fire history, and particularly environmental pollution. Most major protist groups have been used as bioindicators with diatoms, foraminifera and testate amoebae particularly widely studied. To increase uptake of protist bioindication techniques in routine environmental monitoring technique development should consider the needs of stakeholders from an early stage. Papers in this special issue reflect the diversity of both protist life and possible bioindicator applications

Testate amoebae as palaeohydrological proxies in Sürmene Aǧaçbaşi Yaylasi Peatland (Northeast Turkey)

Testate amoebae as palaeohydrological proxies in sürmene ağaçbaşi yaylasi peatland (northeast Turkey) Testate amoebae are unicellular micro-organisms whose hydrological sensitivity and good preservation in peats make them valuable proxies for past peatland surface wetness, and therefore climate. Previous testate amoebae transfer functions have been spatially restricted with no studies from Asia. To derive a transfer function, a sequence of samples was extracted from an ombrotrophic peatland in Turkey and amoebae counted. The internal structure of the data was explored using principal components analysis and relationships with the environmental data tested by redundancy analyses. Transfer function models were developed using a variety of techniques. As in other regions, depth to water table is the most important control on amoebae community composition. Transfer function performance was initially poor, primarily due to the inclusion of samples from areas of the site that had been heavily affected by peat cutting and had distinctly different amoebae communities. Model performance is improved by selective sample exclusion, reducing jack-knifed root mean square error of prediction to 7.1 cm. The model was tested using an initial palaeoecological data-set. Overlap with the training set was limited, although a hydrological reconstruction using this model produces similar results to a transfer function derived from northern European peatlands. This study provides the first testate amoebae transfer function from Asia and demonstrates that hydrological preferences of many of the key taxa are consistent across a large area of the Northern Hemisphere. The transfer function will allow detailed palaeoclimate reconstruction from this peatland, adding to our knowledge of Holocene climatic change in southwest Asia