A Bayesian molecular clock analysis based on the alignment of the complete coding region of 31 WNV_KUN isolates, and others from GenBank, over a 50 year period.

<p>Colours indicate Australian state of origin: northern Queensland, light green; southern Queensland, dark green; New South Wales, blue; South Australia, red; Western Australia, orange; black, unknown origin. Root-to-tip distances plotted against time (inset) showed a strict linear correlation. Additional genome WNV_KUN genome sequences included in this analysis were obtained from GenBank: KUNCG (D00246), Boort (KT934796), Hu6774 (KT934797), GU0631 (KT934798), GU1009 (KT934799), SA2011 (KT934803), P9974 (KT934800), K68967 (KT934802), K74015 (KT934801), and NSW2012 (KT934804). Posterior probabilities greater than 0.9 are shown.</p

CAI values for WNV_KUN isolates 1960–2012.

<p>CAI values for WNV_KUN isolates 1960–2012.</p

Selection pressure on WNV_KUN strains.

<p>(A) SLAC and (B) IFEL analysis of WNV_KUN polyprotein alignment to detected negative and positive selection pressures measured as normalized <i>dN</i>-<i>dS</i> across the genome. Statistically significant residues (<i>P</i> < 0.05) are shown. Codons 1–3342 (<i>x</i>-axis) represent amino acid residues 92–3433 of the polyprotein sequence.</p

Structure of WNV_KUN methyltransferase (NS5).

<p>(A) Ribbon dimer model (upper) showing fixed substitution residues (space-filled residues: blue, amino acid 49; red, amino acid 101; and green, amino acid 183). The structure rotated 90° toward the viewer is also shown (lower). (B) Space filled model with residues coloured according to flavivirus sequence conservation level with corresponding residues indicated.</p

Structure of WNV_KUN NS3.

<p>(A) Ribbon dimer model (upper) showing fixed substitution residues (space-filled residues: red, amino acid 382; and green, amino acid 465). The structure rotated 90° toward the viewer is also shown (lower). (B) Space filled model with residues coloured according to flavivirus sequence conservation level with corresponding residues indicated.</p

Coghlan et al 2015 fasta files

Fasta files for mitochondrial and plant chloroplast DNA sequenced in this stud