DIGOXIN AND RISK OF DEATH IN ADULTS WITH ATRIAL FIBRILLATION: THE ATRIA-CVRN STUDY

PCR primer sequences for each gene marker amplified and their corresponding annealing temperatures. (DOCX 15 kb

Additional file 1: Figure S1. of Matrix-assisted laser desorption/ionization time of flight mass spectrometry for comprehensive indexing of East African ixodid tick species

Comparison of MALDI-TOF MS spectral profiles, indicating distinct mass peak patterns among the different tick genera Amblyomma, Hyalomma, Rhipicephalus and Rhipicephalus (Boophilus). The spectra illustrated in the figure cover a mass range between 4000 to 18,000 Da. The relative peak intensities are indicated on the y-axis. (PDF 143 kb

Additional file 1: of Evidence for the presence of African swine fever virus in an endemic region of Western Kenya in the absence of any reported outbreak

Results of conventional and real-time PCR. Contains sample ID and sampling location with the results from the conventional PCR assay and the real-time PCR assays using the TaqManÂŽ EZ-RT-PCR Kit and the Life Technologies Path-ID Multiplex One Step Kit. (XLSX 43 kb

Impact On Vocabulary Acquisition On University A1 Efl Students’ Performances Through Games

Las personas han estado usando juegos mayormente con propósitos de ntretenimiento, sin embargo los juegos siempre han tenido una naturaleza educativa intrínseca. debido a esta naturaleza, las personas han creado juegos para propósitos educativos, con un adicional de motivación, atractivo y diversión. consecuentemente, los expertos han observado que los juegos son herramientas excelentes para el aprendizaje. (hamdaqui, khalidi, & bennani, 2014). Este estudio de investigación acción explora los resultados de usar juegos para enseñar vocabulario a estudiantes jóvenes adultos de inglés como idioma extranjero, con bajo nivel del idioma y problemas de motivación para el aprendizaje. Esta investigación comparó los resultados de enseñar vocabulario con juegos educacionales en contraste con el método tradicional.GuayaquilMaestría en Enseñanza del Inglés como Lengua Extranjer

EXA-2017-1S-FÍSICA III-4-Mejora.pdf

Tick counts in Nelore and Holstein bovines for confirmation of resistance and susceptibility to infestations with R. microplus. (DOCX 25 kb

Additional file 2: Figure S2. of Immune and biochemical responses in skin differ between bovine hosts genetically susceptible and resistant to the cattle tick Rhipicephalus microplus

Diagram of the zones where cell counts were made in tick infested skin from bovines: Zone 1: presence of an epidermal rupture, the feeding cavity matching the central bite site and the cement cone that are a big mass at the epidermis surface; zone 2: absence of cement cone and presence of inflammatory cells in the dermis surrounding the bite site; zone 3: absence of cement cone, borderline infiltration of inflammatory cells. (TIF 1740 kb

Genes encoding two <i>Theileria parva</i> antigens recognized by CD8⁺ T-cells exhibit sequence diversity in South Sudanese cattle populations but the majority of alleles are similar to the Muguga component of the live vaccine cocktail

<div><p>East Coast fever (ECF), caused by <i>Theileria parva</i> infection, is a frequently fatal disease of cattle in eastern, central and southern Africa, and an emerging disease in South Sudan. Immunization using the infection and treatment method (ITM) is increasingly being used for control in countries affected by ECF, but not yet in South Sudan. It has been reported that CD8⁺ T-cell lymphocytes specific for parasitized cells play a central role in the immunity induced by ITM and a number of <i>T</i>. <i>parva</i> antigens recognized by parasite-specific CD8⁺ T-cells have been identified. In this study we determined the sequence diversity among two of these antigens, Tp1 and Tp2, which are under evaluation as candidates for inclusion in a sub-unit vaccine. <i>T</i>. <i>parva</i> samples (<i>n</i> = 81) obtained from cattle in four geographical regions of South Sudan were studied for sequence polymorphism in partial sequences of the Tp1 and Tp2 genes. Eight positions (1.97%) in Tp1 and 78 positions (15.48%) in Tp2 were shown to be polymorphic, giving rise to four and 14 antigen variants in Tp1 and Tp2, respectively. The overall nucleotide diversity in the Tp1 and Tp2 genes was π = 1.65% and π = 4.76%, respectively. The parasites were sampled from regions approximately 300 km apart, but there was limited evidence for genetic differentiation between populations. Analyses of the sequences revealed limited numbers of amino acid polymorphisms both overall and in residues within the mapped CD8⁺ T-cell epitopes. Although novel epitopes were identified in the samples from South Sudan, a large number of the samples harboured several epitopes in both antigens that were similar to those in the <i>T</i>. <i>parva</i> Muguga reference stock, which is a key component in the widely used live vaccine cocktail.</p></div

Additional file 2: Table S2. of Complete genome sequence of Staphylococcus aureus, strain ILRI_Eymole1/1, isolated from a Kenyan dromedary camel

The 16S rRNA sequences of the type strains of genus Staphylococcus used in phylogenetic tree (Fig. 1). (DOC 47 kb

Multiple amino acid sequence alignment of Tp1 and Tp2 antigen variants present in cattle from South Sudan.

<p>(A) Multiple sequence alignment of the four Tp1 antigen variants. Antigen variants Var-1, -3 and -9 were first described by Pelle et al. (2011) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171426#pone.0171426.ref017" target="_blank">17</a>]. (B) Multiple sequence alignment of 14 Tp2 antigen variants. The naming of the antigen variants follows the nomenclature by Pelle et al. (2011) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171426#pone.0171426.ref017" target="_blank">17</a>]. Antigen variants Var-1, -2 and -5 were first described by Pelle et al. (2011) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171426#pone.0171426.ref017" target="_blank">17</a>]. The CD8⁺ T-cell target epitopes are boxed and the polymorphic residues in the epitopes are shown in red. The frequency of each variant amongst the samples tested is indicated in square brackets. Residues conserved in all sequences are identified below the alignment (*). The shaded flanking regions are equivalent to the positions of the secondary (nested) PCR primers, and are not included in estimations of the percentage of the residues that are conserved.</p

Map of South Sudan showing the sampling sites.

<p>The four areas where cattle samples were collected are colour coded as follows: Bor = Blue; Juba = Orange; Yei = Green; Kajo keji = Red. Numbers in brackets indicate the number of cattle sampled at each site.</p