The Southern Colonial Backcountry: Interdisciplinary Perspectives on Frontier Communities

https://trace.tennessee.edu/utk_early-american/1014/thumbnail.jp

Doing more with less: productivity or starvation? The intellectual asset health check

The recent wave of savings in public service expenditure comes at the risk of creating starved workplaces, depleted of intellectual assets. This paper examines the perils of starved workplaces and how to avoid them. Organizations that nurture their intellectual assets were found to outperform their peers with 13.3% higher productivity. These organizations created a ‘win–win situation’, achieving both productivity targets while sustaining high stocks of emotional and human capital

A method for the isolation of human gastric mucous epithelial cells for primary cell culture: A comparison of biopsy vs surgical tissue

We have developed a method for the isolation and growth of normal human gastric mucous epithelial cells using biopsies or surgically resected tissues as the source of the cells. The attachment and growth of cells were dependent upon: (1) cell planting density, ∼50,000 cells/cm 2 ; (2) extracellular matrix (fibronectin); and (3) and the use of a porous filter. In all experiments we found better cells attachment and growth of human gastric mucous cells isolated from surgical specimens compared with those gastric mucous cells isolated from gastric biopsies. The initial cell viability (as measured by Trypan-blue) was the same in both populations of gastric mucous epithelial cells isolated from either gastric biopsies or surgical specimens. After 4–5 days in culture one could detect various amounts of mucin in all the cells using either periodic acid Schiff (PAS) staining or a specific anti-mucin antibody. A similar pattern of much straining was also found in primary cultures of guinea pig gastric mucous epithelial cells. Immunohistochemical staining for chief cells (anti-pepsinogen) or parietal cells (anti-H + /K + ATPasc) in the gastric mucous cuboidal-like epithelial cells with tight junctions, desmosomes,short microvilli, a filamentous terminal web, mucous granules, and basal lamina-like structure. We could not detect the presence of fibroblasts during the 7–9 days that the primary cells were in culture. This cell culture method will prove useful in the isolation of normal human gastric mucous epithelial cells for in vitro studies of gastric mucosal injury and repair.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/43235/1/11022_2004_Article_BF00127904.pd

The Southern Colonial Backcountry: Interdisciplinary Perspectives on Frontier Communities

https://trace.tennessee.edu/utk_early-american/1014/thumbnail.jp

Nyeri abdomen, diare dan muntah = Abdominal pain and diarrhea &vomiting

+hlm.;c

Nyeri abdomen dan diare dan munteh = Abdominal pain and diarrhea and vomiting

+hlm.;c

Nyeri abdomen, diare dan muntah = Abdominal pain and diarrhea &vomiting

+hlm.;c