Calculation and use of an environment's characteristic software metric set

Since both cost/quality and production environments differ, this study presents an approach for customizing a characteristic set of software metrics to an environment. The approach is applied in the Software Engineering Laboratory (SEL), a NASA Goddard production environment, to 49 candidate process and product metrics of 652 modules from six (51,000 to 112,000 lines) projects. For this particular environment, the method yielded the characteristic metric set (source lines, fault correction effort per executable statement, design effort, code effort, number of I/O parameters, number of versions). The uses examined for a characteristic metric set include forecasting the effort for development, modification, and fault correction of modules based on historical data

The Euphorbiaceae of Sonora, Mexico

This publication is an account of the Euphorbiaceae in the state of Sonora, México. Nineteen genera, 143 species, and three additional varieties are recorded for the state; three species expected within the state are also treated. One species of Acalypha and three species and one subspecies of Euphorbia are described. Dichotomous keys for the identification of genera and species are provided. Bibliographic citations, type information, synonyms, brief habit descriptions, times of reproduction, habitat preferences as well as elevational range within Sonora, geographical distribution, and representative specimens are given for each species. When appropriate, notes on uses, taxonomic or nomenclatural problems, and other points of interest are discussed

Should the Surety Stand on Its Equitable Subrogation Rights or File Its Indemnity Agreement under the Uniform Commercial Code?

I. Introduction II. The Hypothetical Case III. The U.C.C. and Equitable Subrogation ... A. The Bank Has Superior Claims to the Contractor\u27s Assets under the U.C.C. ... B. The Doctrine of Equitable Subrogation ... C. The Tension between the U.C.C. and Equitable Subrogation ... 1. Equitable Subrogation Was Not Replaced by the U .C.C ... 2. Weaknesses of Equitable Subrogation ... 3. Potential Uses of the U.C.C. by Sureties IV. Analysis of the Hypothetical ... A. Who Gets the Contract Balance? ... 1. Rights under the U.C.C. ... 2. Completion of Performance ... 3. Governing Law ... 4. Conclusions as to Contract Balances ... B. Who Gets the Materials? ... 1. The Bank Has a Perfected Security Interest in the Materials ... 2. The Owner May Have Rights Superior to the Bank\u27s Interest ... 3. The Surety Has No Enforceable Claims to Materials under Equitable Subrogation ... 4. The Surety Has Problems Even If It Filed Its Indemnity Agreement under the U.C.C. ... 5. Conclusion—Materials ... C. Who Gets the Equipment? ... 1. The Bank Has Superior Rights to the Equipment … 2. Equitable Subrogation Does Not Give the Surety Any Rights to Contractor\u27s Equipment ... 3. Filing under the U.C.C. Would Have Given the Surety Rights in the Equipment ... 4. Conclusion—Equipment V. What Are the Advantages and Disadvantages of Recording the Indemnity Agreement under the U.C.C.? … A. Advantages of Filing under the U.C.C. ... B. Disadvantages of Filing under the U.C.C. ... C. Filing after Default ... D. Filing When Necessary under the Circumstances May Be the Best Solution VI. Conclusio

Evaluation of Vapor Pressure and Ultra-High Vacuum Tribological Properties of Ionic Liquids (2) Mixtures and Additives

Ionic liquids are salts, many of which are typically viscous fluids at room temperature. The fluids are characterized by negligible vapor pressures under ambient conditions. These properties have led us to study the effectiveness of ionic liquids containing both organic cations and anions for use as space lubricants. In the previous paper we have measured the vapor pressure and some tribological properties of two distinct ionic liquids under simulated space conditions. In this paper we will present vapor pressure measurements for two new ionic liquids and friction coefficient data for boundary lubrication conditions in a spiral orbit tribometer using stainless steel tribocouples. In addition we present the first tribological data on mixed ionic liquids and an ionic liquid additive. Post mortem infrared and Raman analysis of the balls and races indicates the major degradation pathway for these two organic ionic liquids is similar to those of other carbon based lubricants, i.e. deterioration of the organic structure into amorphous graphitic carbon. The coefficients of friction and lifetimes of these lubricants are comparable to or exceed these properties for several commonly used space oils

DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli

Background: Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host is hampered by their instability in intracellular environment. To overcome this problem, an RNA of interest can be fused into a stable bacterial RNA for the resulting chimeric construct to accumulate in the cytoplasm to a sufficiently high level. Being supplemented with cost-effective procedures for isolation of the chimera from cells and recovery of the recombinant RNA from stabilizing scaffold, this strategy might become a viable alternative to the existing methods of chemical or enzymatic RNA synthesis. Results: Sequence encoding a 71-nucleotide recombinant RNA was inserted into a plasmid-borne deletion mutant of the Vibrio proteolyticus 5S rRNA gene in place of helix III - loop C segment of the original 5S rRNA. After transformation into Escherichia coli, the chimeric RNA (3譸en aRNA) was expressed constitutively from E. coli rrnB P1 and P2 promoters. The RNA chimera accumulated to levels that exceeded those of the host's 5S rRNA. A novel method relying on liquid solid partitioning of cellular constituents was developed for isolation of total RNA from bacterial cells. This protocol avoids toxic chemicals, and is therefore more suitable for large scale RNA purification than traditional methods. A pair of biotinylated 8-17 DNAzymes was used to bring about the quantitative excision of the 71-nt recombinant RNA from the chimera. The recombinant RNA was isolated by sequence-specific capture on beads with immobilized complementary deoxyoligonucleotide, while DNAzymes were recovered by biotin affinity chromatography for reuse. Conclusions:The feasibility of a fermentation-based approach for manufacturing large quantities of small RNAs in vivo using a "5S rRNA scaffold" strategy is demonstrated. The approach provides a route towards an economical method for the large-scale production of small RNAs including shRNAs, siRNAs and aptamers for use in clinical and biomedical research

DNAzyme-mediated recovery of small recombinant RNAs from a 5S rRNA-derived chimera expressed in Escherichia coli

<p>Abstract</p> <p>Background</p> <p>Manufacturing large quantities of recombinant RNAs by overexpression in a bacterial host is hampered by their instability in intracellular environment. To overcome this problem, an RNA of interest can be fused into a stable bacterial RNA for the resulting chimeric construct to accumulate in the cytoplasm to a sufficiently high level. Being supplemented with cost-effective procedures for isolation of the chimera from cells and recovery of the recombinant RNA from stabilizing scaffold, this strategy might become a viable alternative to the existing methods of chemical or enzymatic RNA synthesis.</p> <p>Results</p> <p>Sequence encoding a 71-nucleotide recombinant RNA was inserted into a plasmid-borne deletion mutant of the <it>Vibrio proteolyticus </it>5S rRNA gene in place of helix III - loop C segment of the original 5S rRNA. After transformation into <it>Escherichia coli</it>, the chimeric RNA (3×<it>pen </it>aRNA) was expressed constitutively from <it>E. coli rrnB </it>P1 and P2 promoters. The RNA chimera accumulated to levels that exceeded those of the host's 5S rRNA. A novel method relying on liquid-solid partitioning of cellular constituents was developed for isolation of total RNA from bacterial cells. This protocol avoids toxic chemicals, and is therefore more suitable for large scale RNA purification than traditional methods. A pair of biotinylated 8-17 DNAzymes was used to bring about the quantitative excision of the 71-nt recombinant RNA from the chimera. The recombinant RNA was isolated by sequence-specific capture on beads with immobilized complementary deoxyoligonucleotide, while DNAzymes were recovered by biotin affinity chromatography for reuse.</p> <p>Conclusions</p> <p>The feasibility of a fermentation-based approach for manufacturing large quantities of small RNAs <it>in vivo </it>using a "5S rRNA scaffold" strategy is demonstrated. The approach provides a route towards an economical method for the large-scale production of small RNAs including shRNAs, siRNAs and aptamers for use in clinical and biomedical research.</p

A Comparison of Two Methods for MRI Classification of At-Risk Tissue and Core Infarction

Objective: To compare how at-risk tissue and core infarction were defined in two major trials that tested the use of MRI in selecting acute stroke patients for endovascular recanalization therapy.Methods: MRIs from 12 patients evaluated for possible endovascular therapy were processed using the methods published from two major trials, MR RESCUE and DEFUSE 2. Specifically, volumes of at-risk tissue and core infarction were generated from each patient’s MRI. MRIs were then classified as to whether or not they met criteria for salvageable tissue: penumbral pattern for MR RESCUE and/or target profile for DEFUSE 2) as defined by each trial.Results: Volumes of at-risk tissue by the two definitions were correlated (p=0.017) while the volumes of core infarct were not (p=0.059). The volume of at-risk tissue was consistently larger when defined by the penumbral pattern than the target profile while the volume of core infarct was consistently larger when defined by the target profile than the penumbral pattern. When these volumes were used to classify the MRI scans, nine out of 12 patients (75%) were classified as having a penumbral pattern, while only 4 out of 12 patients (33%) were classified as having a target profile. Of the 9 patients classified as penumbral pattern, 5 (55%) were classified differently by the target profile.Interpretation: Our analysis found that the MR RESCUE trial defined salvageable tissue in a way which made it more likely for patients be labeled as favorable for treatment. For the cohort of patients examined in this study, had they been enrolled in both trials, most of the patients identified as having salvageable tissue by the MR RESCUE trial would not have been considered to have salvageable tissue in the DEFUSE 2 trial. Caution should be taken in concluding that MRI selection for endovascular therapy is not effective as imaging selection criteria were substantially different between trials

Structure of the subduction system in southern Peru from seismic array data

The subduction zone in southern Peru is imaged using converted phases from teleseismic P, PP, and PKP waves and Pwave tomography using local and teleseismic events with a linear array of 50 broadband seismic stations spanning 300 km from the coast to near Lake Titicaca. The slab dips at 30° and can be observed to a depth of over 200 km. The Moho is seen as a continuous interface along the profile, and the crustal thickness in the back-arc region (the Altiplano) is 75 km thick, which is sufficient to isostatically support the Andes, as evidenced by the gravity. The shallow crust has zones of negative impedance at a depth of 20 km, which is likely the result of volcanism. At the midcrustal level of 40 km, there is a continuous structure with a positive impedance contrast, which we interpret as the western extent of the Brazilian Craton as it underthrusts to the west. V_p/V_s ratios estimated from receiver function stacks show average values for this region with a few areas of elevated V_p/V_s near the volcanic arc and at a few points in the Altiplano. The results support a model of crustal thickening in which the margin crust is underthrust by the Brazilian Shield

ARFGAP1 promotes the formation of COPI vesicles, suggesting function as a component of the coat

The role of GTPase-activating protein (GAP) that deactivates ADP-ribosylation factor 1 (ARF1) during the formation of coat protein I (COPI) vesicles has been unclear. GAP is originally thought to antagonize vesicle formation by triggering uncoating, but later studies suggest that GAP promotes cargo sorting, a process that occurs during vesicle formation. Recent models have attempted to reconcile these seemingly contradictory roles by suggesting that cargo proteins suppress GAP activity during vesicle formation, but whether GAP truly antagonizes coat recruitment in this process has not been assessed directly. We have reconstituted the formation of COPI vesicles by incubating Golgi membrane with purified soluble components, and find that ARFGAP1 in the presence of GTP promotes vesicle formation and cargo sorting. Moreover, the presence of GTPγS not only blocks vesicle uncoating but also vesicle formation by preventing the proper recruitment of GAP to nascent vesicles. Elucidating how GAP functions in vesicle formation, we find that the level of GAP on the reconstituted vesicles is at least as abundant as COPI and that GAP binds directly to the dilysine motif of cargo proteins. Collectively, these findings suggest that ARFGAP1 promotes vesicle formation by functioning as a component of the COPI coat